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Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum

Published online by Cambridge University Press:  11 April 2024

P. Uparanukraw ,
T. Harnnoi ,
A. Dantrakool ,
N. Morakote  and
W. Chaicumpa
P. Uparanukraw*
Affiliation:
Department of Parasitology Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
T. Harnnoi
Affiliation:
Department of Parasitology Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
A. Dantrakool
Affiliation:
Department of Parasitology Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
N. Morakote
Affiliation:
Department of Parasitology Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
W. Chaicumpa
Affiliation:
Department of Microbiology and Immunology Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
*
*Fax: 6653 217144 E-mail: [email protected]
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Abstract

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The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.

Type
Other
Information
Journal of Helminthology , Volume 73 , Issue 3 , March 1999 , pp. 273 - 276
Copyright
Copyright © Cambridge University Press 1999

References

Anantaphruti, M.T. (1989) ELISA for diagnosis of gnatho-stomiasis using antigens from Gnathostoma doloresi and G. spinigerum . Southeast Asian Journal of Tropical Medicine and Public Health 20, 297–304.Google Scholar
Boongird, P., Phuapradit, P., Siridej, N., Chirachariyavej, T., Chuahirun, S. & Vejjajiva, A. (1977) Neurological manifestations of gnathostomiasis. Journal of Neurological Sciences 15, 92–97.Google Scholar
Chaicumpa, W., Ruangkunaporn, Y., Nopparatana, C., Chongsa-Nguan, M., Tapchaisri, P. & Setasuban, P. (1991) Monoclonal antibody to a diagnostic Mr 24,000 antigen of Gnathostoma spinigerum . International Journal for Parasitology 21, 735–738.Google ScholarPubMed
Daengsavang, S. (1980) A monograph on the genus Gnatho-stoma and gnathostomiasis in Thailand . Tokyo, Southeast Asian Medical Information Center.Google Scholar
Dharmkrong-At, A., Migasena, S., Suntharasamai, P., Bunnag, D., Priwan, R. & Sirisinha, S. (1986) Enzyme-linked immunosorbent assay for detection of antibody to Gnathostoma antigen in patients with intermittent cutaneous migratory swelling. Journal of Clinical Microbiology 23, 847–851.CrossRefGoogle ScholarPubMed
Maleewong, W., Morakote, N., Thamasonthi, W., Charuchinda, K., Tesana, S. & Khamboonruang, C. (1988) Serodiagnosis of human gnathostomiasis. Southeast Asian Journal of Tropical Medicine and Public Health 19, 201–205.Google ScholarPubMed
Maleewong, W., Intapan, P.M., Khempila, J., Wongwajana, S., Wongkham, C. & Morakote, N. (1995) Gnathostoma spinigerum: growth and development of third-stage larvae in vitro. Journal of Parasitology 81, 800–803.CrossRefGoogle ScholarPubMed
Nopparatana, C., Setasuban, P., Chaicumpa, W. & Tapchaisri, P. (1991) Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis. International Journal for Parasitology 21, 677–687.CrossRefGoogle ScholarPubMed
Punyagupta, S., Bunnag, T. & Juttijudata, P. (1990) Eosinophilic meningitis in Thailand: clinical and epidemiological characteristics of 162 patients with myeloencephalitis probably caused by Gnathostoma spinigerum . Journal of Neurological Sciences 51, 80–87.Google Scholar
Rusnak, J.M. & Lucey, D.R. (1993) Clinical gnathostomiasis: case report and review of the English-language literature. Clinical Infectious Diseases 16, 33–50.CrossRefGoogle ScholarPubMed
Suntharasamai, P., Desakorn, V., Migasena, S., Bunnag, D & Harinasuta, T. (1985) ELISA for immunodiagnosis of human gnathostomiasis. Southeast Asian . Journal of Tropical Medicine and Public Health 16, 274–279.Google Scholar
Tapchaisri, P., Nopparatana, C., Chaicumpa, W. & Setasubun, P. (1991) Specific antigen of Gnathostoma spinigerum for immunodiagnosis of human gnathostomiasis. International Journal for Parasitology 21, 315–319.CrossRefGoogle ScholarPubMed
Teekhasaenee, C., Ritch, R. & Kanchanaranya, C. (1986) Ocular parasitic infection in Thailand. Review of Infectious Diseases 8, 350–356.CrossRefGoogle ScholarPubMed
Tuntipopipat, S., Chawengkirttikul, R. & Sirisinha, S. (1993) A simplified method for the fractionation of Gnathostoma-specific antigens for serodiagnosis of human gnathostomiasis. Journal of Helminthology 67, 297–304.CrossRefGoogle Scholar