Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-24T03:29:52.275Z Has data issue: false hasContentIssue false

Purification and properties of haemoglobin from Gastrothylax crumenifer (Trematode:Paramphistomatidae)

Published online by Cambridge University Press:  05 June 2009

Jawed Siddiqui
Affiliation:
section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh–202 001, India
Ather H. Siddiqi
Affiliation:
section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh–202 001, India

Abstract

Gastrothylax crumenifer haemoglobin was isolated, purified, chromatographed, and its molecular weight determined on a calibrated Sephadex G–100 column as well as by sodium dodecyl sulphate by the former and 16 500 by the latter method. The oxygen affinity of the pigment (P50O2) was found to be 6·1 mm Hg at pH 7·4.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Andrews, P. (1964) Estimation of molecular weights of proteins by Sephadex gel filtration. Biochemical Journal, 91, 222223.CrossRefGoogle ScholarPubMed
Barrett, J. (1981) Biochemistry of parasitic helminths Macmillan & Co.; London.CrossRefGoogle Scholar
Cain, G. D. (1969) Purification and properties of haemoglobin from Fasciolopsis buski. Journal of Parasitology, 55, 311320.CrossRefGoogle ScholarPubMed
Chappell, L. H. (1980) Physiology of parasites. Blackie: Glasgow, London.Google Scholar
Haider, S. A. & Siddiqi, A. H. (1976) Spectrophotometric analysis of haemoglobins of some digenetic trematodes and their hosts. Journal of Helminthology, 50, 259266.CrossRefGoogle ScholarPubMed
Haider, S. A. & Siddiqi, A. H. (1977) Alkali denaturation of oxyhaemoglobins of some digenetic trematodes and their hosts. Journal of Helminthology, 51, 373378.CrossRefGoogle ScholarPubMed
Keilin, D. & Wang, Y. L. (1946) Haemoglobins of Gastrophilus larvae. Purification and properties. Biochemical Journal, 40, 855866.CrossRefGoogle Scholar
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 211, 680685.CrossRefGoogle Scholar
Lee, D. L. (1965) Uptake of oxygen. In: The physiology ofnematodes. pp. 5659. W. H. Freeman: San Francisco, USA.Google Scholar
Longmuir, I. S. & Chow, J. (1970) Rapid method for determining effect of agents on oxyhaemoglobin dissociation curves. Journal of Applied Physiology, 26, 343345.CrossRefGoogle Scholar
Rose, J. E. & Kaplan, K. L. (1972) Purification, molecular weight, and oxygen equilibrium of hemoglobin from Syngamus trachea, the poultry gapeworm. Journal of Parasitology, 58, 903906.CrossRefGoogle ScholarPubMed
Siddiqui, J. & Siddiqi, A. H. (1986). Fatty acids of phospholipids from three digenetic trematodes of buffalo. Zeitschrift fur Parasitenkunde, 72, 7982.CrossRefGoogle Scholar
Siddiqui, J., Siddiqi, A. H., ITOH, T. & Matsumoto, T. (1985) Characterization of sterols of three digenetic trematodes of buffalo. Molecular and Biochemical Parasitology, 15, 143148.CrossRefGoogle ScholarPubMed
Tuchschmid, P. E., Kunz, P. A. & Wilson, K. J. (1978) Isolation and characterization of the haemoglobin from the lanceolate fluke Dicrocoelium dendriticum. European Journal of Biochemistry, 88, 387394.CrossRefGoogle ScholarPubMed
Von Brand, T. (1973) Biochemistry of parasites. 2nd edit. Academic Press: New York and London.Google Scholar
Von Brand, T. (1979) Biochemistry and physiology of endoparasites. Elsevier/North-Holland Biomedical Press: Oxford and New York.Google Scholar
Wharton, G. W. (1941) The function of respiratory pigments of certain turtle parasites. Journal of Parasitology, 27, 8187.CrossRefGoogle Scholar