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Presence of a conserved domain of GATA transcription factors in Echinococcus granulosus

Published online by Cambridge University Press:  05 June 2009

B. Garat ,
P. Esperón ,
C. Martínez ,
C. Robello  and
R. Ehrlich
B. Garat
Affiliation:
Departamento de Bioquímica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11200, Uruguay
P. Esperón
Affiliation:
Departamento de Bioquímica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11200, Uruguay Cátedra de Biología, Facultad de Química, Universidad de la Republica, Montevideo 11200, Uruguay
C. Martínez
Affiliation:
Departamento de Bioquímica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11200, Uruguay
C. Robello
Affiliation:
Departamento de Bioquímica, Facultad de Medicina, Universidad de la Republica, Montevideo 11200, Uruguay
R. Ehrlich
Affiliation:
Departamento de Bioquímica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11200, Uruguay
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Abstract

In order to characterize GATA transcription factors in Echinococcus granulosus, a PCR-based cloning strategy was developed. Degenerate oligonucleotides were designed for the most conserved sequence in GATA proteins that include 20 amino acids of the zinc domain. A 60 bp fragment was isolated that had high homology among this sequence and those reported in other species. An analogous sequence was obtained by performing the same procedure with DNA from the free living platyhelminth Dugesia tigrina. High stringency Southern blotting experiments confirmed the presence of this sequence in the parasite genome.

Type
Research Note
Information
Journal of Helminthology , Volume 71 , Issue 4 , December 1997 , pp. 355 - 358
Copyright
Copyright © Cambridge University Press 1997

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References

Abel, T., Michelson, A. & Maniatis, T. (1993) A Drosophila GATA family member that binds to Adh regulatory sequences is expressed in the developing fat body. Development 119, 623–633.CrossRefGoogle ScholarPubMed
Bockamp, E.O., McLaughlin, F., Murrell, A. & Green, A.R. (1994) Transcription factors and the regulation of haematopoiesis: lessons from GATA and SCL proteins. Bioessays 16, 481–488.CrossRefGoogle ScholarPubMed
Daniel-Vedele, F. & Caboche, M. (1993) A tobacco cDNA clone encoding a GATA-1 zinc finger protein homologous to regulators of nitrogen metabolism in fungi. Molecular and General Genetics 240, 365–373.CrossRefGoogle ScholarPubMed
Drevet, J.R., Swevers, L. & Iatrou, K. (1995) Developmental regulation of a silkworm gene encoding multiple GATA-type transcription factors by alternative splicing. Journal of Molecular Biology 246, 43–53.Google ScholarPubMed
Evans, T. & Felsenfeld, G. (1989) The erythroid-specific transcription factor Eryfl: a new finger protein. Cell 58, 877–885.CrossRefGoogle ScholarPubMed
Evans, T., Reitman, M. & Felsenfeld, G. (1988) An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes. Proceedings of the National Academy of Sciences of the United States of America 85, 5976–5980.CrossRefGoogle ScholarPubMed
Fu, Y.-H. & Marzluff, G.A. (1990) nit-2, the major regulatory gene of Neurospora crassa, encodes a protein with a putative zinc finger DNA-binding domain. Molecular and Cellular Biology 10, 1056–1065.Google ScholarPubMed
Gregory, R.C., Taxman, D.J., Seshasayee, D., Kensinger, M.H., Bicker, J.J. & Wojchowski, D.M. (1996) Functional interaction of GATA1 with erythroid Kruppel-like factor and Spl at defined erythroid promoters. Blood 87, 1793–1801.CrossRefGoogle Scholar
Kelley, C., Yee, K., Harland, R. & Zon, L.I. (1994) Ventral expression of GATA-1 and GATA-2 in the Xenopus embryo defines induction of haematopoietic mesoderm. Developmental Biology 165, 193–205.CrossRefGoogle Scholar
Kudla, B., Caddick, M.X., Langdon, T., Martinez-Rossi, N.M., Bennett, C.F., Sibley, S., Wayne, Davies R. & Arst, H.N. Jr. (1990) The regulatory gene areA mediating nitrogen metabolite represion in Aspergillus nidulans Mutation affecting specificity of gene activation alter a loop residue of a putative zinc finger. EMBO Journal 9, 1355–1364.CrossRefGoogle Scholar
Lee, M.E., Temizer, D.H., Cifford, J.A. & Quertermous, T. (1991) Cloning of the GATA-binding protein that regulates endothelin-1 gene expression in endothelial cells. Journal of Biological Chemistry 266, 16188–16192.CrossRefGoogle ScholarPubMed
Marchuk, D., Drumm, M., Saulino, A. & Collins, F.C. (1991) Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Research 19, 1154.CrossRefGoogle ScholarPubMed
McManus, D., Knight, M. & Simpson, A.J.G. (1986) Isolation and characterization of nucleic acids from the hydatid organisms, Echinococcus spp. (Cestoda). Molecular and Biochemical Parasitology 16, 251–266.CrossRefGoogle Scholar
McPherson, M.J., Jones, K.M. & Gurr, S.J. (1991) PCR with highly degenerate primers, pp. 171–185 in McPherson, M.J., Quirke, P. & Taylor, G.R. (Eds) PCR a practical approach. Oxford, IRL Press.CrossRefGoogle Scholar
Oliver, G., Vispo, M., Mailhos, A., Martinez, C., Sosa-Pineda, B., Fielitz, W. & Ehrlich, R. (1992) Homeoboxes in flatworms. Gene 121, 337–342.CrossRefGoogle ScholarPubMed
Omichinsky, J.G., Clore, G.M., Schaad, O., Felsenfeld, G., Trainor, C., Appella, E., Stahl, S.J. & Gronenborn, A.M. (1993) NMR Structure of a specific DNA complex of Zn-containing DNA binding domain of GATA-1. Science 261, 438–446.CrossRefGoogle Scholar
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular cloning. A laboratory manual. New York, Cold Spring Harbor Laboratory Press.Google Scholar
Scazzocchio, C. (1990) Of moulds and men, or two fingers are not better than one. Trends in Genetics 6, 311–313.CrossRefGoogle Scholar
Spieth, J., Shim, Y.H., Lea, K., Conrad, R. & Blumenthal, T.(1991) elt-1, an embrionically expressed Caenorhabditis elegans gene homologous to the GATA transcription factor family. Molecular and Cellular Biology 11, 4651–4659.Google Scholar
Thompson, R.C.A. & Lymbery, A.J. (1994) Echinococcus and hydatid disease. Wallingford, CAB International.Google Scholar
Vieille-Grosjean, I. & Huber, P. (1995) Transcription factor GATA-1 regulates human HOXB2 gene expression in erythroid cells. Journal of Biological Chemistry 270, 4544–4550.CrossRefGoogle ScholarPubMed
Voisard, C., Wang, J., McEvoy, J.L., Xu, P. & Leong, S.A. (1993) urbs1, a gene regulating siderophore biosynthesis in Ustilago maydis, encodes a protein similar to the erythroid transcription factor GATA-1. Molecular and Cellular Biology 13, 7091–7100.Google Scholar