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Preliminary Experiments with a Defined Culture Medium for Larval Fasciola hepatica

Published online by Cambridge University Press:  05 June 2009

R. S. V. Pullin
R. S. V. Pullin
Affiliation:
Department of Biology, University of York, Heslington, York*
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Extract

A defined medium is described as a basis for in vitro culture work with larval Fasciola hepatica. This medium, termed BCM, can be quickly made up by using a system of stock solutions. BCM contains inorganic salts, glucose, amino acids, vitamins and antibiotics, but no lipid or proteins. Rediae can be dissected from infected snails for culture, but many appear to be contaminated with bacteria. Large rediae cannot survive in BCM but free immature cercariae can complete their final maturation in vitro. This final maturation, from the 30th to the 35th day after miracidial penetration of donor snails, includes tail growth and appearance of body pigmentation. Cercariae matured in vitro encyst successfully when transferred from BCM to water. Small rediae survive in BCM for 5 days, but show no growth or development measured as dry weight and total nitrogen.

Type
Research Article
Information
Journal of Helminthology , Volume 47 , Issue 2 , June 1973 , pp. 181 - 189
Copyright
Copyright © Cambridge University Press 1973

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