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Preliminary experiments on the in vitro cultivation of an amphistome Orthocoelium scoliocoelium (Trematoda: Digenea)

Published online by Cambridge University Press:  18 November 2009

A. N. Sharma  and
P. N. Sharma
A. N. Sharma
Affiliation:
Department of Zoology, University of Udaipur, Udaipur-313001, India
P. N. Sharma
Affiliation:
Department of Zoology, University of Udaipur, Udaipur-313001, India
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Abstract

A medium containing inorganic salts, vitamins and amino-acids is described for the in vitro cultivation of the amphistome, Orthocoelium scoliocoelium, from the rumen of buffalo. Based on the properties of rumen fluid, this medium promoted and sustained normal egg production, at 37°C temperature and pH 7·4 to 7·8 for 15 days. In contrast to other media used, no patho-physiological alterations except abnormal cytoplasmic lipid, were detected. Since the parasites produced normal eggs, it is concluded that this medium is suitable for short-term cultivation of the parasites.

Type
Research Papers
Information
Journal of Helminthology , Volume 56 , Issue 3 , September 1982 , pp. 169 - 176
Copyright
Copyright © Cambridge University Press 1982

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