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Performance of loop-mediated isothermal amplification (LAMP) for detection of Schistosoma mansoni infection compared with Kato–Katz and real-time PCR

Published online by Cambridge University Press:  18 April 2022

A.F. Allam Open the ORCID record for A.F. Allam [Opens in a new window] ,
M.A.-N. Kamel ,
H.F. Farag ,
H.G. Raheem ,
A.Y. Shehab  and
N.A.-E. Hagras
A.F. Allam*
Affiliation:
Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt
M.A.-N. Kamel
Affiliation:
Department of Biochemistry, Medical Research Institute, University of Alexandria, Alexandria, Egypt
H.F. Farag
Affiliation:
Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt
H.G. Raheem
Affiliation:
Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt
A.Y. Shehab
Affiliation:
Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt
N.A.-E. Hagras
Affiliation:
Department of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria, Egypt
*
Author for correspondence: A.F. Allam, E-mail: [email protected]
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Abstract

The performance of loop-mediated isothermal amplification (LAMP) for detection of Schistosoma mansoni DNA from stool and urine samples in comparison with Kato–Katz and real-time polymerase chain reaction (PCR) was studied. After obtaining informed consent, 50 children participated in the present study and agreed to submit stool and urine samples. Stool samples were examined by Kato–Katz. Both real-time PCR and LAMP techniques were applied on stool and urine samples. The overall prevalence of S. mansoni was 46% in stool and urine samples as detected by the employed techniques, and 90% of cases had light infection intensity. The highest percentage of infection was diagnosed by real-time PCR (44%), followed by Kato–Katz (42%) and LAMP in the stool (36%), while the lowest percentages of infection were diagnosed by real-time PCR and LAMP in urine samples (24% and 14%, respectively). Kato–Katz, real-time PCR and LAMP showed 100% specificity where the sensitivity was 91.3%, 95.7% and 78.3%, respectively, in stool samples. Real-time PCR and LAMP showed lower sensitivity in urine samples. The LAMP assay is a promising technique for S. mansoni diagnosis in endemic countries of moderate and high-intensity infection. Yet, it needs further optimization, particularly in urine samples.

Keywords

Schistosoma mansoniKato–Katzreal-time PCRloop-mediated isothermal amplificationprimary school children
Type
Research Paper
Information
Journal of Helminthology , Volume 96 , 2022 , e28
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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