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Partial characterization and time course analysis of Hymenolepis diminuta coproantigens

Published online by Cambridge University Press:  05 June 2009

J.C. Allan  and
P.S. Craig
J.C. Allan
Affiliation:
Department of Biological Sciences, University of Salford, Salford, M5 4WT, UK
P.S. Craig
Affiliation:
Department of Biological Sciences, University of Salford, Salford, M5 4WT, UK
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Abstract

An analysis of Hymenolepis diminuta specific antigens in infected rat faeces was carried out. Using a capture type antibody sandwich ELISA assay based on a hyperimmune rabbit anti-worm somatic antisera it was demonstrated that, although antigen was present in faeces before patency, the onset of egg production led to a sharp increase in the levels of parasite antigen in the faeces. Levels of antigen in host faeces were independent of worm burden. Parasite eggs did not contribute significantly to faecal antigen levels. Western blot analysis indicated a number of highly specific antigens at around Mr 69,000, Mr 37,000, Mr 50,000 and Mr 27,000 with a low molecular weight smear at between Mr 30,000 and Mr 34,000 present in the faeces of H. diminuta infected rats. Some cross reaction occurred with an antigen of around Mr 66,000 in the faeces of non H. diminuta infected rodents. Antibody activity against this antigen was removed by affinity adsorption of the antibody solution against normal rat faeces.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 2 , June 1994 , pp. 97 - 103
Copyright
Copyright © Cambridge University Press 1994

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