Hostname: page-component-745bb68f8f-cphqk Total loading time: 0 Render date: 2025-01-11T18:58:20.111Z Has data issue: false hasContentIssue false
  • English
  • Français

The origin of the triploid in Paragonimus westermani on the basis of variable regions in the mitochondrial DNA

Published online by Cambridge University Press:  12 April 2024

T. Agatsuma ,
M. Iwagami ,
Y. Sato ,
J. Iwashita ,
S.J. Hong ,
S.Y. Kang ,
L.Y. Ho ,
K.E. Su ,
K. Kawashima  and
T. Abe
T. Agatsuma*
Affiliation:
Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan
M. Iwagami
Affiliation:
Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan
Y. Sato
Affiliation:
Department of Molecular Biology, Akita Prefectural University, Shimo Shinjo, Akita City, Akita 010-0195, Japan
J. Iwashita
Affiliation:
Department of Molecular Biology, Akita Prefectural University, Shimo Shinjo, Akita City, Akita 010-0195, Japan
S.J. Hong
Affiliation:
Department of Parasitology, Chung-Ang University College of Medicine, Heukseok-dong, Tongjak-gu, Seoul 156-756, Korea
S.Y. Kang
Affiliation:
Department of Parasitology, Chung-Ang University College of Medicine, Heukseok-dong, Tongjak-gu, Seoul 156-756, Korea
L.Y. Ho
Affiliation:
People's Hospital, Beijing Medical University, Beijing 100034, China
K.E. Su
Affiliation:
Department of Parasitology, College of Medicine, National Taiwan University, Taipei, Taiwan
K. Kawashima
Affiliation:
School of Health Sciences, Kyusyu University, Fukuoka 812-8582, Japan
T. Abe
Affiliation:
Department of Molecular Biology, Akita Prefectural University, Shimo Shinjo, Akita City, Akita 010-0195, Japan
*
*Fax: +81 88 880 2535 E-mail: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Triploid, parthenogenetic forms of the lungfluke, Paragonimus westermani, occur in Japan, Korea and China. The origin(s) of triploidy has been debated over the years. Sequences of two regions in the mitochondrial DNA, i.e. partial lrRNA (16S), and a portion of the non-coding region, were obtained from natural populations of P. westermani. All triploid individuals (Japan, Korea, China) and a single tetraploid individual (China) had identical sequences in the 16S region studied. Some sequence variation was observed among diploids, with those from Taiwan being distinct from the remainder. Both neighbour joining and parsimony trees using the 16S region placed diploid individuals from southwestern Japan close to the triploids and the tetraploid. The fragment amplified from the mitochondrial non-coding region showed dimorphism. One form (type A) consisted of 239bp comprising two identical tracts of 70bp separated by a tract of 93bp. The second form (Type B) consisted of only a single 70bp tract. All diploid individuals from Taiwan, China and Korea possessed type A, while those from Japan were polymorphic; individuals from Oita and Hyogo had type B, those from Chiba had type A, but both types were found in Mie. On the other hand, all of the triploid individuals and two tetraploid individuals possessed type B. Both the form present in the non-coding region and the 16S sequence suggest an affinity between a south-eastern group of diploid populations in Japan and the triploid form. A possible mechanism responsible for the origin of the triploid is discussed.

Type
Review Article
Information
Journal of Helminthology , Volume 77 , Issue 4 , December 2003 , pp. 279 - 285
Copyright
Copyright © Cambridge University Press 2003

References

Agatsuma, T. (1987) Isozyme studies on Paragonimus westermani from Malaysia and the Philippines. pp. 57–63 in Kawashima, K. (Ed.) Paragonimus in Asia: biology, genetic variation and speciation (I). Fukuoka, Shunpposha Photographic Printing Co. Ltd.Google Scholar
Agatsuma, T. & Habe, S. (1985) Electrophoretic studies on enzymes of diploid and triploid Paragonimus westermani . Parasitology 91, 489–497.CrossRefGoogle ScholarPubMed
Agatsuma, T. & Su, K.E. (1989) Genetic structure of three Taiwan populations of P westermani. pp. 69–71 in Kawashima, K. (Ed.) Paragonimus in Asia: biology, genetic variation and speciation (II). Fukuoka, Shunpposha Photographic Printing Co. Ltd.Google Scholar
Agatsuma, T., Habe, S. & Yokogawa, M. (1987) Population genetic studies on isozymes of Paragonimus westermani from Taiwan and Japan, with special reference to the origin of the triploid P. westermani. Japanese Journal of Parasitology 36, 302–310 (in Japanese with English abstract).Google Scholar
Agatsuma, T., Habe, S., Kawashima, K. & Blas, B.L. (1988) Population genetic studies on isozymes in Paragonimus westermani from the Philippines. Japanese Journal of Parasitology 37, 195–202.Google Scholar
Agatsuma, T., Habe, S. & Ho, L.Y. (1989) Genetic variability of the diploid and triploid of P. westermani in China and their differentiation. pp. 33–59 in Kawashima, K. (Ed.) Paragonimus in Asia: biology, genetic variation and speciation (II). Fukuoka, Shunpposha Photographic Printing Co. Ltd.Google Scholar
Agatsuma, T., Ho, L.Y., Jian, H., Habe, S., Terasaki, K., Shibahara, T., Sugiyama, H., Kim, D.C. & Kawashima, K. (1992) Electrophoretic evidence for a hybrid origin for tetraploid Paragonimus westermani discovered in northeastern China. Parasitology Research 78, 537–538.CrossRefGoogle ScholarPubMed
Agatsuma, T., Yang, L., Kim, D.C. & Yonekawa, H. (1994) Mitochondrial DNA differentiation of Japanese diploid and triploid Paragonimus westermani . Journal of Helminthology 68, 7–11.CrossRefGoogle ScholarPubMed
Blair, D., Agatsuma, T., Watanobe, T., Okamoto, M. & Ito, A. (1997) Geographical genetic structure within the human lung fluke, Paragonimus westermani, detected from DNA sequences. Parasitology 115, 411–417.CrossRefGoogle ScholarPubMed
Blair, D., Xu, Z.B. & Agatsuma, T. (1999) Paragonimiasis and genus Paragonimus . Advances in Parasitology 42, 114–172.Google ScholarPubMed
Boore, J.L. (1999) Animal mitochondrial genomes. Nucleic Acids Research 27, 1767–1780.CrossRefGoogle ScholarPubMed
Hirai, H. & Agatsuma, T. (1991) Triploidy in Paragonimus westermani . Parasitology Today 7, 19–21.CrossRefGoogle ScholarPubMed
Hirai, H., Sakaguchi, Y., Habe, S. & Imai, H.T. (1985) C-banding analysis of six species of lung flukes, Paragonimus spp. (Trematoda: Platyhelminthes), from Japan and Korea. Zeitschrift für Parasitenkunde 71, 617–629.CrossRefGoogle ScholarPubMed
Iwagami, M., Ho, L.Y., Su, K.E., Lai, P.F., Fukushima, M., Nakano, M., Blair, D., Kawashima, K. & Agatsuma, T. (2000) Molecular phylogeographic studies on Paragonimus westermani in Asia. Journal of Helminthology 74, 315–322.CrossRefGoogle ScholarPubMed
Miyazaki, I. (1978) Two types of lung fluke which has been called Paragonimus westermani (Kerbert, 1878). Medical Bulletin of Fukuoka University 5, 251–263 (in Japanese).Google Scholar
Miyazaki, I. (1991) Helminthic zoonoses. International Medical Foundation of Japan, Tokyo.Google Scholar
Tan, Q. & Li, D. (1990) Study on chromosome C-banding in Paragonimus westermani: comparison of the diploid and triploid types (in Chinese). Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 8, 35–37.Google Scholar
Terasaki, K. (1977) Studies on chromosomes of the lung flukes in Japan. Japanese Journal of Parasitology 26, 222–229.Google Scholar
Terasaki, K., Habe, S. & Ho, L.Y. (1989) Ploidy of chromosomes of the lung fluke, Paragonimus westermani, from China – confirmation of tetraploids. pp. 26–33 in Kawashima, K. (Ed.) Paragonimus in Asia: biology, genetic variation and speciation (II). Fukuoka, Shunpposha Photographic Printing Co. Ltd.Google Scholar
Terasaki, K., Habe, S., Ho, L.Y., Jian, H., Agatsuma, T., Shibahara, T., Sugiyama, H. & Kawashima, K. (1995) Tetraploids of the lung fluke Paragonimus westermani found in China. Parasitology Research 81, 627–630.CrossRefGoogle ScholarPubMed
Terasaki, K., Shibahara, T., Noda, Y. & Kayano, H. (1996) The oocyte of triploid fluke receiving intrusion of sperm from a diploid fluke – evidence for the origin of tetraploids in Paragonimus westermani . Journal of Parasitology 82, 947–950.CrossRefGoogle ScholarPubMed
Van Herwerden, L., Blair, D. & Agatsuma, T. (1999) Genetic diversity in parthenogenetic triploid Paragonimus westermani . International Journal for Parasitology 29, 1477–1482.CrossRefGoogle ScholarPubMed