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Opisthorchis viverrini-like liver fluke in birds from Vietnam: morphological variability and rDNA/mtDNA sequence confirmation

Published online by Cambridge University Press:  31 May 2013

T.H. Dao ,
T.G. Nguyen ,
B. Victor ,
S. Gabriël  and
P. Dorny
T.H. Dao
Affiliation:
Department of Parasitology, National Institute of Veterinary Research, Hanoi, Vietnam
T.G. Nguyen
Affiliation:
Ministry of Public Health, Vietnam
B. Victor
Affiliation:
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Belgium
S. Gabriël
Affiliation:
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
P. Dorny*
Affiliation:
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Belgium
*
* E-mail: [email protected]
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Abstract

Flukes were found in the bile ducts of domestic ducks (Anas platyrhynchos), necropsied in the Binh Dinh province of Central Vietnam. Following staining, morphological characteristics of the bird flukes were compatible with Opisthorchis viverrini, although some characteristics differed from those described in specimens collected from mammal hosts. Computation of the phylogenetic trees on the partial sequences of the second internal ribosomal spacer (ITS2) of the ribosomal DNA and cytochrome c oxidase subunit I (COI) markers of the mitochondrial DNA showed close similarity of the ‘bird’ Opisthorchis sp. with O. viverrini. We speculate that these bird flukes are O. viverrini that show intraspecies morphological and molecular variability compared to isolates from mammals. This demonstrates the complex epidemiological situation of opisthorchiasis in Vietnam and urges investigations on the potential of birds as a reservoir host of this zoonotic fluke.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 4 , December 2014 , pp. 441 - 446
Copyright
Copyright © Cambridge University Press 2013 

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