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Oesophagostomum radiatum: inhibition of in vitro development by newborn calf serum

Published online by Cambridge University Press:  05 June 2009

I. J. East  and
C. J. Fitzgerald
I. J. East
Affiliation:
CSIRO, Division of Tropical Animal Production, Long Pocket Laboratories, Private Bag No. 3, P.O., Indooroopilly, Queensland, 4068, Australia
C. J. Fitzgerald
Affiliation:
CSIRO, Division of Tropical Animal Production, Long Pocket Laboratories, Private Bag No. 3, P.O., Indooroopilly, Queensland, 4068, Australia
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Abstract

Oesophagostomum radiatum developed to fourth stage larvae after 14 days in in vitro culture. However, development was totally inhibited if the standard 50% foetal calf serum in the medium was replaced by newborn calf serum. Inhibition did not occur with serum from cattle immune to O. radiatum through natural infection or experimental vaccination irrespective of the titre of specific antibodies to O. radiatum in each serum. The inhibitory action of NCS could be abolished by heat treatment at 56°C for 1 h but not by dialysis or repeated freeze-thawing. The inhibition was not consistent with observed differences in the activity of 19 enzymes in the various sera or the absence of various thiol-containing stimulants of worm development.

Keywords

Oesophagostomum radiatumin vitro cultureinhibition of developmentnewborn calf serum
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 1 , March 1990 , pp. 9 - 14
Copyright
Copyright © Cambridge University Press 1990

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References

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