Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-24T05:03:12.671Z Has data issue: false hasContentIssue false
  • English
  • Français

Observations on the post-mortem migration of nematode larvae and its role in tissue digestion techniques

Published online by Cambridge University Press:  05 June 2009

Paul Prociv
Paul Prociv
Affiliation:
Department of Parasitology, The University of Queensland, St Lucia, Queensland 4067, Australia
Get access Rights & Permissions [Opens in a new window]

Abstract

The importance of larval viability in relation to the success of recovery techniques and to the interpretation of histological and migration studies was tested using mice infected with Toxocara pteropodis, T. canis and T. cati. Little difference was found in total numbers of viable larvae recovered from tissues incubated for 18 hours in 0·85% saline, 2% trypsin or 1% pepsin, but the rates of larval egress did vary. The recovery of dead larvae by digestion techniques was much lower. Larvae migrated in tissues undergoing fixation, which influenced histological findings. Larvae of T. canis did not undergo significant peritoneal migration in acutely-infected mice, but did penetrate their intestinal walls in large numbers within 4 hours of death. The implications of these findings in experimental studies are discussed.

Keywords

Toxocara pteropodisToxocara canisToxocara catiAscaris lumbricoidesdigestion techniquesnematode larvaelarval migrationmous
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 4 , December 1989 , pp. 281 - 286
Copyright
Copyright © Cambridge University Press 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bhowmick, D. K. (1964) Beitragezudem Problem der Wandervege der Ascaridenlarven (Ascaris lumbriocoides Linnee 1758 und Toxocara canis Werner 1782) im experimentellen und naturlichen Wirt. Zeitschrift für Parasitenkunde, 24, 121–168.CrossRefGoogle Scholar
Dubey, J. P. (1968) Migration of Toxocara cati in mice. Tropical and Geographical Medicine, 20, 172–176.Google ScholarPubMed
Jackson, E., Jackson, F. & Smith, W. D. (1984) Comparison of saline incubation and pepsin digestion as methods for recovering Ostertagia circumcincta larvae from the abomasum of sheep. Research in Veterinary Science, 36, 380–381.CrossRefGoogle ScholarPubMed
Johnstone, C., Leventhal, R. & Soulsby, E. J. L. (1978) The spin method for recovering tissue larvae and its use in evaluating C57B1/6 mice as a model for the study of resistance to infection with Ascaris suum. Journal of Parasitology, 64, 1015–1020.CrossRefGoogle Scholar
Mason, K. V., Prescott, C. W., Kelly, W. R. & Waddell, A. H. (1976) Granulomatous encephalomyelitis of puppies due to Angiostrongylus cantonensis. Australian Veterinary Journal, 52, 295.CrossRefGoogle ScholarPubMed
Prociv, P. (1985) Observations on Toxocara pteropodis infections in mice. Journal of Helminthology, 59, 267–275.CrossRefGoogle ScholarPubMed
Prociv, P. (1986) Toxocara pteropodis, T. canis and T. cati infections in guinea pigs. Tropical Biomedicine, 3, 97–106.Google Scholar
Prociv, P. & Brindley, P. J. (1986) Oral, parenteral and paratenic infections of mice with Toxocara pteropodis. International Journal for Parasitology, 16, 471–474.CrossRefGoogle ScholarPubMed
Prociv, P. & Mak, J. W. (1987) Experimental Toxocara pteropodis infections in the long-tailed macaque, Macaca fascicularis. Tropical Biomedicine, 4, 37–45.Google Scholar
Smith, J. W. & Wootten, R. (1975) Experimental studies on the migration of Anisakis sp. larvae (Nematoda: Ascaridida) into the flesh of the herring, Clupea harengus L. International Journal for Parasitology, 5, 133–136.CrossRefGoogle ScholarPubMed
Sprent, J. F. A. (1952) On the migratory behaviour of the larvae of various Ascaris species in white mice: I. Distribution of larvae in tissues. Journal of Infectious Diseases, 90, 165–176.CrossRefGoogle ScholarPubMed