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Neutral lipids in cercariae, encysted metacercariae, and rediae of Echinostoma caproni

Published online by Cambridge University Press:  12 April 2024

C.J. Marsit ,
B. Fried  and
J. Sherma
C.J. Marsit
Affiliation:
Department of Chemistry, Lafayette College, Easton, Pennsylvania, 18042, USA
B. Fried*
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
J. Sherma
Affiliation:
Department of Chemistry, Lafayette College, Easton, Pennsylvania, 18042, USA
*
*Author for correspondence Fax: (610)330 5705 E-mail: [email protected]
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Abstract

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High performance thin-layer chromatography (HPTLC) was used to analyse the neutral lipids in the rediae, cercariae, and encysted metacercariae of Echinostoma caproni from Biomphalaria glabrata snails. Visual observations of the chromatograms showed that the most abundant lipid fraction in all stages was free sterol. Quantification of the free sterol revealed mean weights of 2.7 ± 0.64 ng per redia, 0.53 ± 0.023 ng per cercaria, and 0.081 ± 0.0098 ng per encysted metacercaria. Oil Red O staining of the larval stages confirmed the presence of lipids within the rediae and cercariae but did not show lipids in the encysted metacercariae. The dimunition in neutral lipids from the cercarial to the encysted metacercarial stage does not support a previous observation that fat increases in successive phases of the digenean life cycle.

Type
Research Article
Information
Journal of Helminthology , Volume 74 , Issue 4 , December 2000 , pp. 365 - 367
Copyright
Copyright © Cambridge University Press 2000

References

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