Hostname: page-component-745bb68f8f-cphqk Total loading time: 0 Render date: 2025-01-11T22:16:53.812Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphometric and molecular analyses of two digenean species from the mullet: Haplosplanchnus pachysomus (Eysenhardt, 1892) from Vietnam and Provitellotrema crenimugilis Pan, 1984 from the Russian southern Far East

Published online by Cambridge University Press:  22 April 2015

V.V. Besprozvannykh ,
D.M. Atopkin ,
H.D. Ngo ,
A.V. Ermolenko ,
N.V. Ha ,
N.V. Tang  and
A.Yu. Beloded
V.V. Besprozvannykh
Affiliation:
Institute of Biology and Soil Science, Far-Eastern Branch of Russian Academy of Sciences, Vladivostok, Russia
D.M. Atopkin*
Affiliation:
Institute of Biology and Soil Science, Far-Eastern Branch of Russian Academy of Sciences, Vladivostok, Russia
H.D. Ngo
Affiliation:
Institute of Ecology and Biodiversity, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
A.V. Ermolenko
Affiliation:
Institute of Biology and Soil Science, Far-Eastern Branch of Russian Academy of Sciences, Vladivostok, Russia
N.V. Ha
Affiliation:
Institute of Ecology and Biodiversity, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
N.V. Tang
Affiliation:
Institute of Ecology and Biodiversity, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
A.Yu. Beloded
Affiliation:
Institute of Biology and Soil Science, Far-Eastern Branch of Russian Academy of Sciences, Vladivostok, Russia
*
*Fax: +7 4232310193 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Adults of Haplosplanchnus pachysomus (Eysenhardt, 1829) were found in the intestine of Liza engeli (Bleeker) from the coastal waters of Cat Ba Island, Ha Long Bay, northern Vietnam. Additionally, Provitellotrema crenimugilis Pan, 1984 was discovered in Liza haematocheila (Temminck & Schlegel) from Vostok Bay, Gulf of Peter the Great, southern Far-East Russia. Data concerning morphology, 18S rDNA and 28S rDNA of these worms were obtained.The molecular data confirmed the validity of these species and showed that specimens identified as H. pachysomus are closely related to specimens of H. pachysomus found in Spain, and that P. crenimugilis is closely related to Haplosplanchnus purii. Molecular differentiation of P. crenimugilis and H. purii was 0.92% by combined ribosomal gene sequences that confirmed species validity. Molecular differentiation between P. crenimugilis and H. purii, on the one hand, and H. pachysomus, on the other hand, was much higher, suggesting that the sequence for H. purii in GenBank is for a misidentified species of the genus Provitellotrema Pan, 1984.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 2 , March 2016 , pp. 238 - 244
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmad, J. (1985) Studies on digenetic trematodes of marine fishes from the Arabian Sea off the Panjim coast, Goa, India. Part 48. Revista Iberica de Parasitologica 45, 185–194.Google Scholar
Bray, R.A. (1984) Some helminth parasites of marine fishes and cephalopods of South Africa: Aspidogastrea and the digenean families Bucephalidae, Haplosplanchnidae, Mesometridae and Fellodistomidae. Journal of Natural History 18, 271–292.Google Scholar
Cribb, T.H., Bray, R.A., Littlewood, D.T.J., Pichelin, S. & Herniou, E.A. (2001) Relationships of the Digenea – evidence from molecules and morphology. pp. 186–193 in Littlewood, D.T.J. & Bray, R.A. (Eds) Interrelationships of platyhelminthes. London, Taylor & Francis.Google Scholar
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using bootstrap. Evolution 39, 783–791.CrossRefGoogle ScholarPubMed
Gajevskaja, A.V. & Dmitrieva, E.V. (1992) [Haplosplanchnus pachysomus (Trematoda: Haplosplanchnidae), a parasite of mullets from Black Sea]. Parazitologiya 26, 385-388 (in Russian).Google Scholar
Gupta, V. & Ahmad, J. (1979) Digenetic trematodes of marine fishes. VIII. On four new species of the genus Haplosplanchnus Looss, 1902 from marine fishes of Puri, Orissa. Helminthologia 16, 185–193.Google Scholar
Huelsenbeck, J.P. (2000) Mr Bayes: Bayesian inference of phylogeny. Department of Biology, University of Rochester, Rochester, New York.Google Scholar
Huelsenbeck, J.P., Ronquist, F., Nielsen, R. & Bollback, J.P. (2001) Bayesian inference of phylogeny and its impact on evolutionary biology. Science 294, 2310–2314.CrossRefGoogle ScholarPubMed
Littlewood, D.T.J. & Olson, P.D. (2001) Small subunit rDNA and the platyhelminthes: signal, noise, conflict and compromise. pp. 262–278 in Littlewood, D.T.J. & Bray, R.A. (Eds) Interrelationships of platyhelminthes. London, Taylor & Francis.Google Scholar
Madhavi, R. (2005) Superfamily Haplosplanchnoidea Poche, 1926. pp. 175–191 in Jones, A., Bray, R.A. & Gibson, D.I. (Eds) Keys to the Trematoda, vol. 2 . Wallingford, CAB International.Google Scholar
Nahhas, F.M., Rhodes, D.Y. & Seeto, J. (1997) Digenetic trematodes of marine fishes from Suva, Fiji, family Haplosplanchnidae Poche, 1926: description of new species, a review and an update. The University of the South Pa. Suva, Fiji Islands, .Google Scholar
Olson, P.D., Cribb, T.H., Tkach, V.V., Bray, R.A. & Littlewood, D.T.J. (2003) Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33, 733–755.Google Scholar
Pan, J.P. (1984) Two new genera and three new species of Hemiuridae and Maseniidae from Kwangtung fishes. pp. 125–132 in Institute of Hydrobiology Academia Sinica (Ed.) Parasitic organisms of freshwater fish of China. Beijing China, Agricultural Publishing House.Google Scholar
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.CrossRefGoogle ScholarPubMed
Skrjabin, K.I. & Guschanskaja, L.Ch. (1955) Subfamily Haplosplonchninae Skrjabinet Guschanskaja, 1955. pp. 582–592 in Skrjabin, K.I. (Ed.) Trematodes of animals and man. Principles of trematodology. Moscow, Academy of Science of USSR (in Russian).Google Scholar
Srivastava, H.D. (1939) The morphology and systematic relationship of two new distomes of the family Haplosplanchnidae Poche, 1926, from Indian marine food-fishes. The Indian Journal of Veterinary Science and Animal Husbandry 9, 67–71.Google Scholar
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729.CrossRefGoogle ScholarPubMed
Tkach, V.V., Littlewood, D.T.J., Olson, P.D., Kinsella, J.M. & Swiderski, Z. (2003) Molecular phylogenetic analysis of the Microphalloidea Ward, 1901 (Trematoda: Digenea). Systematic Parasitology 56, 1–15.CrossRefGoogle ScholarPubMed
Truett, G.E. (2006) Preparation of genomic DNA from animal tissues. pp. 33–46 in Kieleczawa, J. (Ed.) The DNA book: Protocols and procedures for the modern molecular biology. Sudbury, Massachusetts, Jones & Bartlett Publisher.Google Scholar
Zhukov, E.V. (1971) [New trematodes of marine and freshwater fishes from the basins of the Japanese and Yellow seas]. Parazitologiya 2, 155–161 (in Russion).Google Scholar