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Morphological and molecular characterization of two new species of the genus Aporcelinus Andrássy, 2009 (Nematoda, Dorylaimida, Aporcelaimidae) from the USA, with new insights on the phylogeny of the genus

Published online by Cambridge University Press:  11 December 2018

S. Álvarez-Ortega Open the ORCID record for S. Álvarez-Ortega [Opens in a new window] ,
S.A. Subbotin  and
R. Peña-Santiago
S. Álvarez-Ortega*
Affiliation:
Departamento de Biología y Geología, Física y Química Inorgánica, Universidad Rey Juan Carlos, Campus de Móstoles, 28933-Madrid, España Plant Pest Diagnostics Center, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, CA 95832-1448, USA
S.A. Subbotin
Affiliation:
Plant Pest Diagnostics Center, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, CA 95832-1448, USA
R. Peña-Santiago
Affiliation:
Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus ‘Las Lagunillas’ s/n, Edificio B3, 23071- Jaén, Spain
*
Author for correspondence: S. Álvarez-Ortega, E-mail: [email protected]
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Abstract

Two new species of the genus Aporcelinus from the USA are described and illustrated. Aporcelinus floridensis sp. n. is characterized by its 1.12–1.52 mm long body, lip region offset by marked constriction and 14.5–17.0 μm broad with perioral liplets, odontostyle 16.5–20.0 μm at its ventral side and 1.1–1.2 times the lip region diameter, neck 316–395 μm long, pharyngeal expansion occupying 43–48% of total neck length, uterus simple and 33–56 μm long or 0.8–1.2 times the corresponding body diameter, V = 48–54, female tail conical (36–49 μm long, c = 27–41, c’ = 1.2–2.0) with finely rounded terminus and no hyaline portion, and male absent. Aporcelinus paolae sp. n. is characterized by its 1.29–1.80 mm long body, lip region offset by marked constriction and 14–16 μm broad, odontostyle 15–17 μm at its ventral side and 1.0–1.1 times the lip region diameter, neck 314–397 μm long, pharyngeal expansion occupying 43–53% of total neck length, uterus tripartite and 128–164 μm long or 2.6–3.6 times the corresponding body diameter, V = 53–57, female tail conical (30–39 μm long, c = 40–51, c’ = 1.1–1.3) with finely rounded terminus and variably re-curved dorsad, male tail conical (27–36 μm, c = 39–59, c’ = 0.9–1.2), ventrally straight and dorsally convex, spicules 48–54 μm long, and 7–9 irregularly spaced ventromedian supplements lacking hiatus. The analyses of the D2-D3 expansion segments of 28S rRNA (LSU) gene sequences of the two new species confirmed the monophyly of the genus, based upon currently available data, showing a close relationship between the genera Aporcelinus and Makatinus, and justified the placement of Aporcelaimellus, Makatinus and Aporcelinus under the subfamily Aporcelaimellinae.

Keywords

Bayesian inferencedescriptionD2-D3 expansion segmentsLSU ribosomal RNA genemaximum likelihoodmolecularmorphologymorphometricsSEMtaxonomy
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e22
Copyright
Copyright © Cambridge University Press 2018 

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