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Morphological and molecular characterization of Mermisnigrescens Dujardin, 1842 (Nematoda: Mermithidae) parasitizing the introduced European earwig (Dermaptera: Forficulidae) in New Zealand

Published online by Cambridge University Press:  06 February 2014

B. Presswell ,
S. Evans ,
R. Poulin  and
F. Jorge
B. Presswell*
Affiliation:
Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand
S. Evans
Affiliation:
Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand
R. Poulin
Affiliation:
Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand
F. Jorge
Affiliation:
Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand CIBIO - Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661Vairão, Portugal Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, R. Campo Alegre, s/n, 4169-007Porto, Portugal
*
*Fax: (+64)(0)3 4797584 E-mail: [email protected]
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Abstract

Parasitic nematodes of the family Mermithidae were found to be infecting the introduced European earwig Forficula auricularia (Dermaptera: Forficulidae) in Dunedin, South Island, New Zealand. Adult females were later collected from various garden plants while depositing eggs. These mermithid specimens were identified morphologically as Mermis nigrescens Dujardin, 1842. A genetic distance of 0.7% between these specimens and a M. nigrescens isolate from Canada (18S rRNA gene), suggests that they have diverged genetically, but there are currently no available comparable sequences for the European M. nigrescens. Two additional nuclear fragments were also amplified, the 28S rRNA and the ribosomal DNA first internal transcribed spacer (ITS1), providing a basis for future studies. Bearing in mind the morphological similarity with other reported M. nigrescens and the lack of sequence data from other parts of the world, we retain the name M.nigrescens, and suggest that the species may be found to represent a complex of cryptic species when more worldwide data are available. Herein, we present a brief description of the post-parasitic worms and adult females, along with an inferred phylogeny using 18S rRNA gene sequences.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 3 , May 2015 , pp. 267 - 276
Copyright
Copyright © Cambridge University Press 2014 

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References

Anderson, T.J.C., Blouin, M.S. & Beech, R.N. (1998) Population biology of parasitic nematodes: applications of genetic markers. Advances in Parasitology 41, 219–283.Google Scholar
Baker, G.L. & Capinera, J.L. (1997) Nematodes and nematomorphs as control agents of grasshoppers and locusts. Memoires of the Entomological Society of Canada 171, 157–211.Google Scholar
Baker, G.L. & Poinar, G.O. (1986) Mermis quirindiensis n. sp. (Nematoda: Mermithidae), a parasite of locusts and grasshoppers (Orthoptera: Acrididae) in south-eastern Australia. Revue de nématologie 9, 125–134.Google Scholar
Baker, G.L. & Poinar, G.O. (1988) A description of the male and redescription of female Mermis athysanota Steiner, 1921 (Nematoda: Mermithidae). Revue de nématologie 11, 343–350.Google Scholar
Barker, G.M., Pottinger, R.P. & Addison, P.J. (1989) Population dynamics of the Argentine stem weevil (Listronotus bonariensis) in pastures of Waikato, New Zealand. Agriculture, Ecosystems & Environment 26, 79–115.Google Scholar
Baylis, H.A. (1944) Observations on the nematode Mermis nigrescens and related species. Parasitology 36, 122–132.CrossRefGoogle Scholar
Baylis, H.A. (1947) The larval stages of the nematode Mermis nigrescens . Parasitology 38, 10–16.Google Scholar
Bell, N., Davis, L., Sarathchandra, S., Barratt, B., Ferguson, C. & Townsend, R. (2005) Biodiversity of indigenous tussock grassland sites in Otago, Canterbury and the central North Island of New Zealand II. Nematodes. Journal of the Royal Society of New Zealand 35, 303–319.CrossRefGoogle Scholar
Blaxter, M.L., De Ley, P., Garey, J.R., Liu, L.X., Scheldeman, P., Vierstraete, A., Vanfleteren, J.R., Mackey, L.Y., Dorris, M., Frisse, L.M., Vida, J.T. & Thomas, W.K. (1998) A molecular evolutionary framework for the phylum Nematoda. Nature Methods 392, 71–75.Google Scholar
Burr, A.J., Hunt, P., Wagar, D.R., Dewilde, S., Blaxter, M.L., Vanfleteren, J.R. & Moens, L. (2000) A hemoglobin with an optical function. Journal of Biological Chemistry 275, 4810–4815.CrossRefGoogle ScholarPubMed
Buxton, J.H. & Madge, D.S. (1976) The evaluation of the European earwig (Forficula auricularia) as a predator of the damson-hop aphid (Phorodon humuli). I. Feeding experiments. Entomologia Experimentalis et Applicata 19, 109–114.Google Scholar
Carroll, D.P. & Hoyt, S.C. (1984) Augmentation of European earwigs (Dermaptera: Forficulidae) for biological control of apple aphid (Homoptera: Aphididae) in an apple orchard. Journal of Economic Entomology 77, 738–740.Google Scholar
Cherry, T., Szalanski, A.L., Todd, T.C. & Powers, T.O. (1997) The internal transcribed spacer region of Belonolaimus (Nemata: Belonolaimidae). Journal of Nematology 29, 23–29.Google Scholar
Christie, J. (1937) Mermis subnigrescens, a nematode parasite of grasshoppers. Journal of Agricultural Research 55, 353–364.Google Scholar
Cobb, N. (1926) The species of Mermis: a group of very remarkable nemas infesting insects. Journal of Parasitology 13, 66–72.CrossRefGoogle Scholar
Craig, D. (1963) The occurrence of nematodes in the family Blepharoceridae (Diptera). New Zealand Entomologist 3, 25.CrossRefGoogle Scholar
Crainey, J.L., Wilson, M.D. & Post, R.J. (2009) An 18S ribosomal DNA barcode for the study of Isomermis lairdi, a parasite of the blackfly Simulium damnosum s.l. Medical and Veterinary Entomology 23, 238–244.Google Scholar
Crowcroft, P.W. (1947) Notes on the occurrence of the nematode Mermis nigrescens Dujardin and its effect on the common earwig in Tasmania. Papers and Proceedings of the Royal Society of Tasmania 1947, 59–62.Google Scholar
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9, 772.Google Scholar
Drummond, A., Ashton, B., Buxton, S., Cheung, M., Cooper, A., Duran, C., Field, M., Heled, J., Kearse, M. & Markowitz, S. (2011) Geneious, Version 5.4. Auckland, New Zealand, Biomatters Ltd.Google Scholar
Dujardin, F. (1842) Memoire sur le Gordius et les Mermis. Comptes rendus de l'Académie des Sciences 15, 117–119.Google Scholar
Felsenstein, J. (1985) Phylogenies and the comparative method. The American Naturalist 125, 1–15.Google Scholar
Fonseca, G., Derycke, S. & Moens, T. (2008) Integrative taxonomy in two free-living nematode species complexes. Biological Journal of the Linnaean Society 94, 737–753.Google Scholar
Guindon, S. & Gascuel, O. (2003) A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Systematic Biology 52, 696–704.Google Scholar
Hagmeier, A. (1912) Beiträge zur Kenntnis der Mermithiden. 1. Biologische Notizen und systematische Beschreibung einiger alter und neuer Arten. Zoologische Jahrbücher (Systematik) 32, 521–612.Google Scholar
Hoberg, E.P., Monsen, K.J., Kutz, S. & Blouin, M.S. (1999) Structure, biodiversity, and historical biogeography of nematode faunas in Holarctic ruminants: morphological and molecular diagnoses for Teladorsagia boreoarcticus n. sp. (Nematoda: Ostertagiinae), a dimorphic cryptic species in muskoxen (Ovibos moschatus). Journal of Parasitology 85, 910–934.Google Scholar
Holterman, M., van der Wurff, A., van den Elsen, S., van Megen, H., Bongers, T., Holovachov, O., Bakker, J. & Helder, J. (2006) Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 1792–1800.Google Scholar
Hoy, J.M. (1952) Note on the occurrence of nematodes in larvae of Odontria zealandica White. New Zealand Entomologist 1, 5–6.Google Scholar
Huelsenbeck, J.P. & Ronquist, F. (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics 17, 754–755.Google Scholar
Hung, G.-C., Chilton, N.B., Beveridge, I., Zhu, X.Q., Lichtenfels, J.R. & Gasser, R.B. (1999) Molecular evidence for cryptic species within Cylicostephanus minutus (Nematoda: Strongylidae). International Journal for Parasitology 29, 285–291.Google Scholar
Johnson, A.A. & Kleve, M.G. (2010) A new mermithid species (Nematoda) from chironomid imagos eclosing from Lake Ozawindib in northern Minnesota. Journal of Parasitology 96, 1173–1175.CrossRefGoogle ScholarPubMed
MacFarlane, R.P. (2011) Order Dermaptera: Earwigs. pp. 256–260 in Gordon, D.P. (Ed.) New Zealand inventory of biodiversity. Volume Two, Kingdom Animalia: Chaetognatha, ecdysozoa, ichnofossils. Christchurch, Canterbury University Press.Google Scholar
Meldal, B.H., Debenham, N.J., De Ley, P., De Ley, I.T., Vanfleteren, J.R., Vierstraete, A.R., Bert, W., Borgonie, G., Moens, T., Tyler, P.A., Austen, M.C., Blaxter, M.L., Rogers, A.D. & Lambshead, P.J. (2007) An improved molecular phylogeny of the Nematoda with special emphasis on marine taxa. Molecular Phylogenetics and Evolution 42, 622–636.Google Scholar
Mueller, T.F., Blommers, L.H.M. & Mois, P.J.M. (1988) Earwig (Forficula auricularia) predation on the woolly apple aphid, Eriosoma lanigerum . Entomologia Experimentalis et Applicata 47, 145–152.Google Scholar
Neilson, R., Ye, W., de Oliveira, C.M., Hubschen, J., Robbins, R.T., Brown, D.J.F. & Szalanski, A.L. (2004) Phylogenetic relationships of selected species of Longidoridae (Nematoda: Longidoridae) from North America inferred from 18S rDNA gene sequence data. Helminthologia 41, 209–215.Google Scholar
Nickle, W. (1972) A contribution to our knowledge of the Mermithidae (Nematoda). Journal of Nematology 4, 113.Google Scholar
Olia, M., Ahmad, W., Araki, M., Minaka, N., Oba, H. & Okada, H. (2008) Actus salvadoricus Baqri and Jairajpuri (Mononchida: Mylonchulidae) from Japan with comment on the phylogenetic position of the genus Actus based on 18S rDNA sequences. Nihon Senchu Gakkai Shi 38, 57–69.Google Scholar
Poinar, G.O. (1983) Nematode parasites of invertebrates. pp. 161–202 in Poinar, G.O. (Ed.) The natural history of nematodes. Englewood Cliffs, NJ, Prentice-Hall.Google Scholar
Poinar, G.O. (1990) Austromermis n. gen. and Blepharomermis n. gen. (Mermithidae: Nematoda) from New Zealand Simuliidae and Bleparoceridae (Diptera). Revue de nématologie 13, 395–402.Google Scholar
Poinar, G.O. & Early, J. (1990) Aranimermis giganteus n. sp. (Mermithidae: Nematoda), a parasite of New Zealand mygalomorph spiders (Araneae: Arachnida). Revue de nématologie 13, 403–410.Google Scholar
Poinar, G.O. & Jackson, T.A. (1992) Psammomermis canterburiensis n. sp. (Mermithidae: Nematoda) parasitic in the New Zealand grass grub, Costelytra zealandica (Scarabaeidae: Coleoptera). Fundamental and Applied Nematology 15, 43–47.Google Scholar
Poinar, G.O., Rémillet, M. & van Waerebeke, D. (1978) Mermis changodudus sp. n. (Mermithidae) a nematode parasite of Heteronychus beetles (Scarabaeidae) in Madagascar. Nematologica 24, 100–104.Google Scholar
Poinar, G.O., Latham, A.D.M. & Poulin, R. (2002) Thaumamermis zealandica n. sp. (Mermithidae: Nematoda) parasitising the intertidal marine amphipod Talorchestia quoyana (Talitridae: Amphipoda) in New Zealand, with a summary of mermithids infecting amphipods. Systematic Parasitology 53, 227–233.Google Scholar
Powers, T. (2004) Nematode molecular diagnostics: from bands to barcodes. Annual Review of Phytopathology 42, 367–383.Google Scholar
Ross, J.L., Ivanova, E.S., Spiridonov, S.E., Waeyenberge, L., Moens, M., Nicol, G.W. & Wilson, M.J. (2010) Molecular phylogeny of slug-parasitic nematodes inferred from 18S rRNA gene sequences. Molecular Phylogenetics and Evolution 55, 738–743.Google Scholar
Sato, T., Watanabe, K., Tamotsu, S., Ichikawa, A. & Schmidt-Rhaesa, A. (2012) Diversity of nematomorph and cohabiting nematode parasites in riparian ecosystems around the Kii Peninsula, Japan. Canadian Journal of Zoology 90, 829–838.CrossRefGoogle Scholar
Scott, R.R. (1984) Berry fruit pests. pp. 13–27 in Scott, R.R. (Ed.) New Zealand pest and beneficial insects. Christchurch, Lincoln University College of Agriculture.Google Scholar
Smith, W.W. (1898) Forficula auricularia in New Zealand. Entomologist's Monthly Magazine 34, 162.Google Scholar
St-Onge, M., LaRue, B. & Charpentier, G. (2008) A molecular revision of the taxonomic status of mermithid parasites of black flies from Quebec (Canada). Journal of Invertebrate Pathology 98, 299–306.Google Scholar
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 2731–2739.Google Scholar
Thomas, W.P. (1989) Forficula auricularia L., European earwig (Dermaptera: Forficulidae). pp. 201–205 in Cameron, P.J., Hill, R.L., Bain, J. & Thomas, W.P. (Eds) A review of biological control of invertebrate pests and weeds in New Zealand, 1874–1987. Wallingford, UK, CAB International.Google Scholar
Vrain, T.C., Wakarchuk, D.A., Levesque, A.C. & Hamilton, R.I. (1992) Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology 15, 563–573.Google Scholar
Wang, J.-Y., Xu, F., Liu, X.-S. & Wang, G.-X. (2007) Molecular phylogeny of entomopathogenic nematodes (Mermithidae) inferred from DNA sequences of 18S rDNA, 28S rDNA and CO1 genes. Acta Zoologica Sinica 53, 835–844.Google Scholar
Ward, D.F. (1998) A preliminary investigation into the host-parasite relationship of an alpine cricket (Orthoptera: Rhaphidophoridae) from New Zealand. Journal of Orthoptera Research 7, 99–101.Google Scholar
Werle, E., Schneider, C., Renner, M., Völker, M. & Fiehn, W. (1994) Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Research 22, 4354–4355.Google Scholar
Whiting, M.F. (2002) Mecoptera is paraphyletic: multiple genes and phylogeny of Mecoptera and Siphonaptera. Zoologica Scripta 31, 93–104.Google Scholar
Williams, J., Townsend, C. & Poulin, R. (2001) Mermithid nematode infections and drift in the mayfly Deleatidium spp. (Ephemeroptera). Journal of Parasitology 87, 1225–1227.Google Scholar
Wilson, W. (1971) Nematode occurrence in Ontario earwigs (Nematoda: Dermaptera). The Canadian Entomologist 103, 1045–1048.Google Scholar
Yeates, G.W. & Buckley, T.R. (2009) First records of mermithid nematodes (Nematoda: Mermithidae) parasitising stick insects (Insecta: Phasmatodea). New Zealand Journal of Zoology 36, 35–39.Google Scholar
Zhang, Z., Schwartz, S., Wagner, L. & Miller, W. (2000) A greedy algorithm for aligning DNA sequences. Journal of Computational Biology 7, 203–214.Google Scholar

Presswell Supplementary Material

Mermis nigrescens female climbing to the tip of fern frond in the summer after rainfall. Note the anterior tip region exhibiting the yellowish colour characteristic of the female (ocellar haemoglobin), and the brownish eggs visible in the posterior body.

Download Presswell Supplementary Material(Video)
Video 207.5 MB