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Morphological and histochemical observations on the digestive system of Cotylophoron cotylophorum

Published online by Cambridge University Press:  05 June 2009

V. R. Parshad  and
S. S. Guraya
V. R. Parshad
Affiliation:
Department of Zoology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana 141004, India
S. S. Guraya
Affiliation:
Department of Zoology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana 141004, India
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Abstract

The amphistome Cotylophoron cotylophorum feeds entirely on semidigested rumenal contents (plant material living bacteria, and protozoa). Morphological and histochemical studies have been made of the digestive system. Two types of cells occur in the caecal epithelium, the first type were larger and cuboidal with granular cytoplasm containing a high amount of RNA and proteins. The second smaller type were fewer, and contained pyknotic nuclei; their cytoplasm was vacuolated. The cells of the first type bear microvilli at the luminal surface. The surface coat consists of saliva resistant acetylated acid mucopolysaccharides containing hyaluronic acid with carboxyl polyanions. Intense activities of acid phosphatase, glucose-6-phosphatase, and ATPase, and moderate activities of nonspecific esterase and lipase are localized at the brush border. The functional significance of the surface carbohydrates and the hydrolytic enzymes has been discussed in relation to digestion and absorption of nutrients.

Type
Research Papers
Information
Journal of Helminthology , Volume 52 , Issue 4 , December 1978 , pp. 327 - 333
Copyright
Copyright © Cambridge University Press 1978

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References

REFERENCES

Bennett, C. E. (1975) Fasciola hepatica. Development of caecal epithelium during migration in the mouse. Experimental Parasitology, 37, 426–441.CrossRefGoogle ScholarPubMed
Bennet, G. (1970) Migration of glycoprotein from Golgi apparatus to cell coat in columnar cells of the duodenal epithelium. Journal of Cell Biology, 45, 668–673.CrossRefGoogle Scholar
Bogitsh, B. J. (1975a) Cytochemical observations on the gastrodermis of digenetic trematodes. Transactions of American Microscopical Society, 94, 524–528.CrossRefGoogle ScholarPubMed
Bogitsh, B. J. (1975b) Cytochemistry of gastrodermal autophagy following starvation in Schistosoma mansoni. Journal of Parasitology, 61, 237–248.CrossRefGoogle ScholarPubMed
Cook, G. W. M. and Stoddart, R. W. (1973) Surface carbohydrates of eukaryotic cell. Academic Press: London and New York.Google Scholar
Crane, R. K. (1969) A perspective of digestive absorptive function. American Journal of Clinical Nutrition, 22, 242–249.CrossRefGoogle ScholarPubMed
Dawes, B. (1962) A histological study of the caecal epithelium of Fasciola hepatica L. Parasitology, 52, 483–493.CrossRefGoogle Scholar
Dike, S. and Read, C. (1971) Tegumentary phosphohydrolases of Hymenolepis diminuta. Journal of Parasitology, 57, 81–87.CrossRefGoogle ScholarPubMed
Erasmus, E. A. (1972) The biology of trematodes. Edward Arnold: London.Google Scholar
Gomori, G. (1952) Microscopic histochemistry. University of Chicago Press: Chicago.Google Scholar
Gresson, R. A. R. and Threadgold, L. T. (1959) A light and electron microscope study of the epithelial cells of the gut of Fasciola hepatica L. Journal Biophysics and Biochemical Cytology, 6, 157–162.CrossRefGoogle ScholarPubMed
Gupta, A. N. (1970) Histochemical localization of alkaline phosphatase and its functional significance in the gut of digenetic trematode. Acta Biologica Acadamie Science; Hungary, 21, 91–97.Google Scholar
Halton, D. W. (1967a) Studies on phosphatase activity in Trematoda. Journal of Parasitology, 53, 46–54.CrossRefGoogle ScholarPubMed
Halton, D. W. (1967b) Observations on the nutrition of digenetic trematodes. Parasitology, 57, 639–660.CrossRefGoogle ScholarPubMed
Halton, D. W. and Stranock, S. D. (1976) The fine structure and histochemistry of the caecal epithelium of Calicotyle kroyeri (Monogenea: Monopisthocotylea). International Journal of Parasitology, 6, 253–263.CrossRefGoogle ScholarPubMed
Hanna, R. E. B. (1975) Fasciola hepatica: An electron microscope autoradiographic study of protein synthesis and secretion by gut cells in tissue slices. Experimental Parasitology, 38, 167–180.CrossRefGoogle ScholarPubMed
Humason, G. L. (1972) Animal tissue technique. W. H. Freeman and Company: San Francisco.Google Scholar
Ito, S. (1969) Structure and function of the glycocalyx. Federation Proceedings, 28, 12–25.Google ScholarPubMed
Kaplow, L. S. and Burstone, M. S. (1964) Cytochemical demonstration of acid phosphatase in hematopoietic cells in health and various hematological disorders using azo-dye technique. Journal of Histochemistry and cytochemistry, 12, 805–811.CrossRefGoogle Scholar
Knutton, S., Jackson, D., Graham, J. M., Micklem, K. J. and Pastesnak, C. A. (1976) Microvilli and cell swelling. Nature, 262, 52–53.CrossRefGoogle ScholarPubMed
Love, R. and Liles, R. H. (1959) Differentiation of nucleoproteins by inactivation of protein bound amino groups and staining with toluidine blue ammonium molybdate. Journal of histochemistry and cytochemistry, 7, 164–181.CrossRefGoogle ScholarPubMed
Lumsden, R. D. (1975) Surface ultrastructure and cytochemistry of parasitic helminths. Experimental Parasitology, 37, 267–339.CrossRefGoogle ScholarPubMed
Mazia, D., Brewer, P. A. and Alfret, M. (1957) The cytochemical staining and measurement of protein with mercuric bromphenol blue. Biological Bulletin, 104, 57–67.CrossRefGoogle Scholar
Menzies, D. W. (1966) Staining of small lymphoid nucleoli paraffin sections by anniline azure B. Stain Technology, 41, 165–168.CrossRefGoogle ScholarPubMed
Morris, G. P. (1973) The fine structure of the caecal epithelium of Megalodiscus temperatus. Canadian Journal of Zoology, 51, 457–460.CrossRefGoogle ScholarPubMed
Mowry, R. W. (1963) The special value of methods that color both acidic and bis-hydroxyl groups in the histochemical study of mucins with revised direction for colloidal iron stain, the use of alcian blue 8GX and their combination with PAS reaction. Annals of New York Academy of Sciences, 106, 402–423.CrossRefGoogle Scholar
Pappas, P. W. and Read, C. P. (1975) Membrane transport in helminth parasites: A review. Experimental Parasitology, 37, 469–530.CrossRefGoogle ScholarPubMed
Parshad, V. R. and Guraya, S. S. (1976) Comparative histochemical observations on the lipids in the immature and mature stages of Cotylophoron cotylophorum (Paramphistomatidae: Digenea). Journal of Helminthology, 50, 11–15.CrossRefGoogle ScholarPubMed
Pearse, A. G. E. (1968) Histochemistry, theoretical and applied. Churchill: London.Google Scholar
Quintarelli, G. and Dellovo, M. C. (1965) The chemical and histochemical properties of alcian blue. IV. Further studies on the method for the identification of acid glycosaminoglycans. Histochemie, 5, 196–209.CrossRefGoogle ScholarPubMed
Quintarelli, G. and Dellovo, M. C. (1969) Studies on the exocrine secretions. Histochemical investigation of major salivary gland of exotic animals. Histochemie, 19, 199–223.CrossRefGoogle ScholarPubMed
Robinson, G. and Threadgold, L. T. (1975) Electron microscope studies of Fasciola hepatica XII. The fine structure of the gastrodermis. Experimental Parasitology, 37, 20–36.CrossRefGoogle ScholarPubMed
Scott, J. E. and Dorling, J. (1965) Differential staining of acid glycosaminoglycans (mucopoly-saccharides) by alcian blue in salt solutions. Histochemie, 5, 221–233.CrossRefGoogle Scholar
Spicer, S. S., Hom, R. G. and Leppi, T. J. (1967) Histochemistry of connective tissue mucopoly-saccharides. In The Connective tissue. International Academy of Pathology Monograph No. 7, Baltimore.Google Scholar
Taylor, K. B. and Clefford, C. (1973) Differentiation between acetylated and non-acetylated PAS-reactive mucosubstances. Stain Technology, 48, 142–143.CrossRefGoogle Scholar
Thorsell, W. and Bjorkman, N. (1965) Morphological and biochemical studies on absorption and secretion in the alimentary tract of Fasciola hepatica L. Journal of Parasitology, 51, 217–223.CrossRefGoogle ScholarPubMed
Wotton, R. M. and Sogandares-Bernal, F. (1963) A report on the occurrences of microvillus structures on the caeca of certain trematodes. Parasitology, 53, 157–161.CrossRefGoogle Scholar