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Molecular characterization of pouched amphistome parasites (Trematoda: Gastrothylacidae) using ribosomal ITS2 sequence and secondary structures

Published online by Cambridge University Press:  07 April 2011

S. Ghatani ,
J.A. Shylla ,
V. Tandon ,
A. Chatterjee  and
B. Roy
S. Ghatani
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong793 022, Meghalaya, India
J.A. Shylla
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong793 022, Meghalaya, India
V. Tandon*
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong793 022, Meghalaya, India
A. Chatterjee
Affiliation:
Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong793 022, Meghalaya, India
B. Roy
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong793 022, Meghalaya, India
*
*Fax: +91 364 2550 300 E-mail: [email protected]
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Abstract

Members of the family Gastrothylacidae (Trematoda: Digenea: Paramphistomata) are parasitic in ruminants throughout Africa and Asia. In north-east India, five species of pouched amphistomes, namely Fischoederius cobboldi, F. elongatus, Gastrothylax crumenifer, Carmyerius spatiosus and Velasquezotrema tripurensis, belonging to this family have been reported so far. In the present study, the molecular phylogeny of these five gastrothylacid species is derived using the second internal transcribed spacer (ITS2) sequence and secondary structure analyses. ITS2 sequence analysis was carried out to see the occurrence of interspecific variations among the species. Phylogenetic analyses were performed for primary sequence data alone as well as the combined sequence-structure information using neighbour-joining and Bayesian approaches. The sequence analysis revealed that there exist considerable interspecific variations among the various gastrothylacid fluke species. In contrast, the inferred secondary structures for the five species using minimum free energy modelling showed structural identities, in conformity with the core four-helix domain structure that has been recently identified as common to almost all eukaryotic taxa. The phylogenetic tree reconstructed using combined sequence–structure data showed a better resolution, as compared to the one using sequence data alone, with the gastrothylacid species forming a monophyletic group that is well separated from members of the other family, Paramphistomidae, of the amphistomid flukes group. The study provides the molecular characterization based on primary sequence data of the rDNA ITS2 region of the gastrothylacid amphistome flukes. Results also demonstrate the phylogenetic utility of the ITS2 sequence–secondary structure data for inferences at higher taxonomic levels.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 1 , March 2012 , pp. 117 - 124
Copyright
Copyright © Cambridge University Press 2011

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