Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-02T20:36:27.809Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular and phylogenetic analyses of the liver amphistome Explanatum explanatum (Creplin, 1847) Fukui, 1929 in ruminants from Bangladesh and Nepal based on nuclear ribosomal ITS2 and mitochondrial nad1 sequences

Published online by Cambridge University Press:  22 June 2016

U.K. Mohanta ,
H.B. Rana ,
B. Devkota  and
T. Itagaki
U.K. Mohanta
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3–18–8, Morioka 020–8550, Japan Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Yanagido 1–1, Gifu 501–1193, Japan
H.B. Rana
Affiliation:
Institute of Agriculture and Animal Science, Tribhuvan University, Kirtipur, Chitwan, Nepal
B. Devkota
Affiliation:
Center for Biotechnology, Agriculture and Forestry University, Rampur, Chitwan, Nepal
T. Itagaki*
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3–18–8, Morioka 020–8550, Japan Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Yanagido 1–1, Gifu 501–1193, Japan
*
*Fax: +81 19 621 6219 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Explanatum explanatum flukes, liver amphistomes of ruminants, cause significant economic loss in the livestock industry by inducing severe liver damage. A total of 66 flukes from 26 buffaloes and 7 cattle in four different geographic areas of Bangladesh and 20 flukes from 10 buffaloes in the Chitwan district of Nepal were subjected for analysis. The sequences (442 bp) of the second internal transcribed spacer (ITS2) of ribosomal DNA and the variable fragments (657 bp) of mitochondrial nicotinamide dehydrogenase subunit 1 (nad1) of E. explanatum flukes from Bangladesh and Nepal were analysed. The aim of this study was molecular characterization of the flukes and to elucidate their origin and biogeography. In the ITS2 region, two genotypes were detected among the flukes from Bangladesh, while flukes from Nepal were of only one genotype. Phylogenetic analyses inferred from the nad1 gene revealed that at least four divergent populations (groups I–IV) are distributed in Bangladesh, whereas two divergent populations were found to be distributed in Nepal. Fst values (pairwise fixation index) suggest that Bangladeshi and Nepalese populations of group I to IV are significantly different from each other; but within groups III and IV, the populations from Bangladesh and Nepal were genetically close. This divergence in the nad1 gene indicates that each lineage of E. explanatum from diverse geography was co-adapted during the multiple domestication events of ruminants. This study, for the first time, provides molecular characterization of E. explanatum in Bangladesh and Nepal, and may provide useful information for elucidating its origin and dispersal route in Asia.

Type
Research Papers
Information
Journal of Helminthology , Volume 91 , Issue 4 , July 2017 , pp. 497 - 503
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmedullah, F., Akbor, M., Haider, M.G., Hossain, M.M., Khan, M.A.H.N.A., Hossain, M.I. & Shanta, I.S. (2007) Pathological investigation of liver of the slaughtered buffaloes in Barisal district. Bangladesh Journal of Veterinary Medicine 5, 81–85.Google Scholar
Alim, M.A., Islam, M.K. & Mondal, M.M.H. (2005) A cross sectional study on Fasciola gigantica and Gigantocotyle explanatum burdens in naturally infected buffaloes in Bangladesh. Bangladesh Journal of Veterinary Medicine 3, 39–44.Google Scholar
Bandelt, H.J., Forster, P. & Rohl, A. (1999) Median-joining network for inferring intraspecific phylogenies. Molecular Biology and Evolution 16, 37–48.CrossRefGoogle Scholar
Bowles, J., Hope, M., Tiu, W.U., Liu, S.X. & McManus, D.P. (1993) Nuclear and mitochondrial markers highly conserved between Chinese and Philippine Schistosoma japonicum . Acta Tropica 55, 217–229.CrossRefGoogle ScholarPubMed
Bowles, J., Blair, D. & McManus, D.P. (1995) A molecular phylogeny of the human schistosomes. Molecular Phylogenetics and Evolution 4, 103–109.Google Scholar
Chen, S., Lin, B., Baig, M., Mitra, B., Lopes, R.J., Santos, A.M., Magee, D.A., Azevedo, M., Tarroso, P., Sasazaki, S., Ostrowski, S., Mahgoub, O., Chaudhuri, T.K., Zhang, Y., Costa, V., Royo, L.J., Goyache, F., Luikart, G., Boivin, N., Fuller, D.Q., Mannen, H., Bradley, D.G. & Beja–Pereira, A. (2010) Zebu cattle are an exclusive legacy of the South Asia Neolithic. Molecular Biology and Evolution 27, 1–6.CrossRefGoogle ScholarPubMed
Eduardo, E.L. (1982) Techniques for examining paramphistomes. Journal of Helminthology 56, 117–119.Google Scholar
Excoffier, L. & Lischer, H.E.L. (2010) Arlequin suite ver. 3.5: a new series of programs to perform population genetic analyses under Linux and Windows. Molecular Ecology Resources 10, 564–567.CrossRefGoogle ScholarPubMed
Haque, M., Mohan, C. & Ahmad, I. (2011) Natural trematode infection in liver of water buffalo (Bubalis bubalis): histopathological investigation. Journal of Parasitic Diseases 35, 50–53.CrossRefGoogle ScholarPubMed
Ichikawa, M., Kondoh, D., Bawn, S., Maw, N.N., Htun, L.L., Thein, M., Gyi, A., Sunn, K., Katakura, K. & Itagaki, T. (2013) Morphological and molecular characterization of Explanatum explanatum from cattle and buffaloes in Myanmar. Journal of Veterinary Medical Science 75, 309–314.CrossRefGoogle ScholarPubMed
Itagaki, T. & Tsutsumi, K. (1998) Triploid form of Fasciola in Japan: genetic relationships between Fasciola hepatica and Fasciola gigantica determined by ITS-2 sequence of nuclear rDNA. International Journal for Parasitology 28, 777–781.Google Scholar
Itagaki, T., Tsumagari, N., Tsutsumi, K. & Chinone, S. (2003) Discrimination of three amphistome species by PCR–RFLP based on rDNA ITS2 markers. Journal of Veterinary Medical Science 65, 931–933.Google Scholar
Jones, A. (2005) Superfamily Paramphistomoidea Fischoeder, 1901. pp. 221–327 in Jones, A., Bray, R.A. & Gibson, D.I. (Eds) Keys to the Trematoda. Wallingford, CABI Publishing.Google Scholar
Kulasiri, C. & Seneviratne, R.D. (1956) Gigantocotyle explanatum Creplin (Trematoda: Paramphistomidae) infection of the liver of the buffalo in Ceylon. Journal of Comparative Pathology 66, 83–88.CrossRefGoogle ScholarPubMed
Kumar, S., Nagarajan, M., Sandhu, J.S., Kumar, N. & Behl, V. (2007) Phylogeography and domestication of Indian river buffalo. BMC Evolutionary Biolology 7, 186.Google Scholar
Le, T.H., Blair, D. & McManus, D.P. (2000) Mitochondrial genomes of human helminths and their use as markers in population genetics and phylogeny. Acta Tropica 77, 243–256.Google Scholar
Lee, S.U. & Huh, S. (2004) Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis . Korean Journal of Parasitology 42, 145–148.CrossRefGoogle ScholarPubMed
Librado, P. & Rozas, Z. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 1451–1452.Google Scholar
Lotfy, W.M., Brant, S.V., Ashmawy, K.I., Devkota, R., Mkoji, G.M. & Loker, E.S. (2010) A molecular approach for identification of paramphistomes from Africa and Asia. Veterinary Parasitology 174, 234–240.CrossRefGoogle ScholarPubMed
Mazahery, Y., Razmyar, J. & Hoghooghi–Rad, N. (1994) Explanatum explanatum (Creplin, 1847) Fukui, 1929, in buffaloes in the Ahwaz area, southwest Iran. Veterinary Parasitology 55, 149–153.CrossRefGoogle ScholarPubMed
Morgan, J.A. & Blair, D. (1998) Mitochondrial ND1 gene sequences used to identify echinostome isolates from Australia and New Zealand. International Journal for Parasitology 28, 493–502.Google Scholar
Näsmark, K.E. (1937) A revision of the Trematode family Paramphistomidae. Zoologiska Bidrag frdn Upsala 16, 301–566.Google Scholar
Park, G.M., Im, K.I. & Yong, T.S. (2003) Phylogenetic relationship of ribosomal ITS2 and mitochondrial COI among diploid and triploid Paragonimus westermani isolates. Korean Journal of Parasitology 41, 45–55.CrossRefGoogle ScholarPubMed
Poulin, R. & Mouillot, D. (2003) Host introductions and the geography of parasite taxonomic diversity. Journal of Biogeography 30, 837–845.Google Scholar
Rinaldi, L., Perugini, A.G., Capuano, F., Fenizia, D., Musella, V., Veneziano, V. & Cringoli, G. (2005) Characterization of the second internal transcribed spacer of ribosomal DNA of Calicophoron daubneyi from various hosts and locations in southern Italy. Veterinary Parasitology 131, 247–253.CrossRefGoogle ScholarPubMed
Semyenova, S.K., Morozova, E.V., Chrisanfova, G.G., Gorokhov, V.V., Arkhipov, I.A., Moskvin, A.S., Movsessyan, S.O. & Ryskov, A.P. (2006) Genetic differentiation in eastern European and western Asian populations of the liver fluke, Fasciola hepatica, as revealed by mitochondrial nad1 and cox1 genes. Journal of Parasitology 92, 525–530.Google Scholar
Sey, O. (1991) CRC handbook of the zoology of amphistomes. 1st edn. Boca Raton, Florida, USA, CRC Press.Google Scholar
Singh, K.S. (1958) A redescription and life-history of Gigantocotyle explanatum (Creplin, 1847) Nasmark, 1937 (Trematoda: Paramphistomidae) from India. Journal of Parasitology 44, 210–224.Google Scholar
Sorensen, E., Drew, A.C., Brindley, P.J., Bogh, H.O., Gasser, R.B., Qian, B.Z., Chiping, Q. & McManus, D.P. (1998) Variation in the sequence of a mitochondrial NADH dehydrogenase I gene fragment among six natural populations of Schistosoma japonicum from China. International Journal for Parasitology 28, 1931–1934.Google ScholarPubMed
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729.Google Scholar
Van Damme, D. (2014) Gyraulus convexiusculus. The IUCN Red List of threatened species. Available at http://dx.doi.org/10.2305/IUCN.UK.2014-1.RLTS.T166681A42421590.en (accessed 8 March 2016).Google Scholar
Supplementary material: File

Mohanta supplementary material S1

Supplementary Table

Download Mohanta supplementary material S1(File)
File 19.2 KB
Supplementary material: File

Mohanta supplementary material S2

Supplementary Table

Download Mohanta supplementary material S2(File)
File 15.4 KB