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Levels of ecdysteroid-like material in adults of Nippostrongylus brasiliensis during the intestinal phase

Published online by Cambridge University Press:  05 June 2009

B. Nembo ,
P. Duie ,
M. Garcia ,
P. Breton ,
P. Gayral ,
P. Porcheron  and
F. Goudey-Perriere
B. Nembo
Affiliation:
Laboratoire de Biologie et Contrôle des Organismes Parasites, Faculté de Pharmacie, Rue J. B. Clément, 92296 Châtenay-Malabry Cedex, France
P. Duie
Affiliation:
Laboratoire de Biochimie et Physiologie du Développement, URA CNRS 686, Dèpartement de Biologie, Ecole Normale Supérieure, 46, rue d'ulm, 75230 Paris Cedex 05, France
M. Garcia
Affiliation:
Laboratoire de Biochimie et Physiologie du Développement, URA CNRS 686, Dèpartement de Biologie, Ecole Normale Supérieure, 46, rue d'ulm, 75230 Paris Cedex 05, France
P. Breton
Affiliation:
Laboratoire de Biologie et Contrôle des Organismes Parasites, Faculté de Pharmacie, Rue J. B. Clément, 92296 Châtenay-Malabry Cedex, France
P. Gayral
Affiliation:
Laboratoire de Biologie et Contrôle des Organismes Parasites, Faculté de Pharmacie, Rue J. B. Clément, 92296 Châtenay-Malabry Cedex, France
P. Porcheron
Affiliation:
Laboratoire de Biochimie et Physiologie du Développement, URA CNRS 686, Dèpartement de Biologie, Ecole Normale Supérieure, 46, rue d'ulm, 75230 Paris Cedex 05, France
F. Goudey-Perriere*
Affiliation:
Laboratoire de Biologie et Contrôle des Organismes Parasites, Faculté de Pharmacie, Rue J. B. Clément, 92296 Châtenay-Malabry Cedex, France
*
*Author for correspondence.
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Abstract

Total ecdysteroid-like immunoreactive material was assayed and quantified in adults of the parasitic nematode N. brasiliensis during the intestinal phase in the rat in order to detect possible physiological fluctuations in titre. Worms of the same sex isolated from one rat were pooled in order to quantify ecdysteroids using enzyme immunoassay (EIA). The concentration of ecdysteroids fluctuated during adult life according to the sex and the age of the parasite. Important differences of levels of ecdysteroid-like compounds between the two sexes of parasites were noted particularly at 128 and 168 h post-infection. The peak at 128 h, present in female, but not in male worms, corresponds to the time of egg-laying. Following HPLC-EIA analysis, the presence of ecdysone, 20-hydroxyecdysone and apolar ecdysteroid-like material was ascertained during this peak. The physiological role and the possible origin of ecdysteroids in this nematode are discussed.

Keywords

ecdysteroidsNippostrongylus brasiliensisratNematodahormones
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 4 , December 1993 , pp. 305 - 315
Copyright
Copyright © Cambridge University Press 1993

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