Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-07T07:03:50.022Z Has data issue: false hasContentIssue false
  • English
  • Français

Lack of negative effects of the biological control agent Duddingtonia flagrans on soil nematodes and other nematophagous fungi

Published online by Cambridge University Press:  01 December 2015

C.A. Saumell ,
A.S. Fernández ,
F. Echevarria ,
I. Gonçalves ,
L. Iglesias ,
M.F. Sagües  and
E.M. Rodríguez
C.A. Saumell*
Affiliation:
Área de Parasitología y Enfermedades Parasitarias, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), Facultad de Ciencias Veterinarias (FCV), Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Paraje Arroyo Seco, B7001, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Tandil, Argentina
A.S. Fernández
Affiliation:
Área de Parasitología y Enfermedades Parasitarias, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), Facultad de Ciencias Veterinarias (FCV), Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Paraje Arroyo Seco, B7001, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Tandil, Argentina
F. Echevarria
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) CPPSUL, C.P. 242, 96401-970 Bagé, Brazil
I. Gonçalves
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) CPPSUL, C.P. 242, 96401-970 Bagé, Brazil
L. Iglesias
Affiliation:
Área de Parasitología y Enfermedades Parasitarias, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), Facultad de Ciencias Veterinarias (FCV), Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Paraje Arroyo Seco, B7001, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Tandil, Argentina
M.F. Sagües
Affiliation:
Área de Parasitología y Enfermedades Parasitarias, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), Facultad de Ciencias Veterinarias (FCV), Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Paraje Arroyo Seco, B7001, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Tandil, Argentina
E.M. Rodríguez
Affiliation:
Área de Bioestadística y Epidemiología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), Facultad de Ciencias Veterinarias (FCV), Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Paraje Arroyo Seco, B7001, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Tandil, Argentina
*
*Fax: +54 249 4385850 ext 251 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The possible environmental effects of the massive use of Duddingtonia flagrans for controlling sheep nematodes were evaluated in two regions. Non-supplemented faeces and faeces from sheep supplemented with D. flagrans were deposited three times on pasture plots and samples were collected 7 and 14 days post-deposition. Samples were cultured in agar-water (2%) with Panagrellus spp. to recover D. flagrans and other nematophagous fungi, and soil nematodes were extracted using Baermann funnels and counted. No significant differences in the populations of soil nematodes and fungi colonizing sheep faeces (P > 0.05) were observed between supplemented and non-supplemented groups, except in one sample. The topsoil in contact with the faeces was sampled 1–4 months post-deposition, revealing that, with one exception, D. flagrans did not persist in soil beyond 2 months post-deposition. Duddingtonia flagrans does not affect faecal colonization by other fungi and soil nematodes and, once deployed on pasture, does not survive for long periods in the environment.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 6 , November 2016 , pp. 706 - 711
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cooke, R.C. & Godfrey, B.E.S. (1964) A key to the nematode-destroying fungi. Transactions of the British Mycological Society 47, 61–74.Google Scholar
Facultad de Ciencias Veterinarias (2002) Acta Original sobre Bienestar Animal. Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina. Available at http://www.vet.unicen.edu.ar/html/Comite%20Bienestar%20Animal/ComiteBienestarAnimalActas.html (accessed accessed 15 September 2015).Google Scholar
Faedo, M., Larsen, M., Dimander, S.O., Yeates, G.W., Höglund, J. & Waller, P.J. (2002) Growth of the fungus Duddingtonia flagrans in soil surrounding feces deposited by cattle or sheep fed the fungus to control nematode parasites. Biological Control 23, 64–70.Google Scholar
Fernández, A.S. & Saumell, C.A. (2012) Biological control of parasites in veterinary medicine. pp. 309–340 in Paz-Silva, A. & Sol, M. (Eds) Fungi: Types, environmental impact and role in disease. Hauppauge, New York, Nova Science Publishers.Google Scholar
Hammer, Ø., Harper, D.A.T. & Ryan, P.D. (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4.Google Scholar
Hay, F.S., Niezen, J.H., Miller, C., Bateson, L. & Robertson, H. (1997) Infestation of sheep dung by nematophagous fungi and implication for the control of free-living stages of gastro-intestinal nematodes. Veterinary Parasitology 70, 247–254.Google Scholar
IndexFungorum.org (2014) Index Fungorum Partnership: Mycology Section, Royal Botanical Gardens Kew; Mycology Group, Landcare Research-NZ; Institute of Microbiology, Chinese Academy of Science. Available at http://www.indexfungorum.org / (accessed 3 October 2015).Google Scholar
Knox, M.R., Josh, P.F. & Anderson, L.J. (2002) Deployment of Duddingtonia flagrans in an improved pasture system: dispersal, persistence, and effects on free-living soil nematodes and microarthropods. Biological Control 24, 176–182.Google Scholar
Larsen, M. (1999) Biological control of helminths. International Journal for Parasitology 29, 139–146.Google Scholar
Ministério da Agricultura, Pecuária e Abastecimento. (2008) Instruçáo Normativa Nro. 56, Recommendations of good practices for the welfare of farm animals and animals of economic interest. Available at http://www.jusbrasil.com.br/diarios/888827/pg-5-secao-1-diario-oficial-da-uniao-dou-de-07-11-2008 (accessed accessed 19 November 2015).Google Scholar
Nansen, P., Larsen, M., Grønvold, J., Wolstrup, J., Zorn, A. & Henriksen, S.A. (1995) Prevention of clinical trichostrongylidosis in calves by strategic feeding with the predacious fungus Duddingtonia flagrans . Parasitology Research 81, 371–374.Google Scholar
Paraud, C., Lorrain, R., Pors, I. & Chartier, C. (2012) Administration of the nematophagous fungus Duddingtonia flagrans to goats: an evaluation of the impact of this fungus on the degradation of faeces and on free-living nematodes. Journal of Helminthology 86, 95–103.Google Scholar
Rubner, A. (1996) Revision of predacious Hyphomycetes in the Dactylella–Monacrosporium complex. Studies in Mycology 39, 1–134.Google Scholar
Sanyal, P.K., Sarkar, A.K., Patel, N.K., Pal, S. & Mandal, S.C. (2008) Evaluation of Chhattisgarh isolate of nematode trapping fungus Duddingtonia flagrans for their short-term environmental impact: studies on fungal spread and faecal decomposition. Journal of Veterinary Parasitology 22, 5–8.Google Scholar
SAS Institute Inc. (1989) SAS/STAT user's guide, version 6, vol. 1. 4th edn. Cary, North Carolina, SAS Institute.Google Scholar
Saumell, C.A. & Padilha, T. (2000) Influence of weather and time of deposition on sheep faeces colonization by nematophagous fungi in the Mata region of Minas Gerais State, Brazil. Applied Soil Ecology 14, 63–70.Google Scholar
Saumell, C.A., Fernández, A.S., Fusé, L.A., Rodríguez, M., Sagüés, M.F. & Iglesias, L.E. (2015) Nematophagous fungi from decomposing cattle faeces in Argentina. Revista Iberoamericana de Micología 32, 252–256.Google Scholar
Soulsby, E.J.L. (1982) Helminths, arthropods and protozoa of domesticated animals. 7th edn. 809 pp. London, Baillière Tindall.Google Scholar
Villareal, H, Álvarez, M., Córdoba, S., Escobar, F., Fagua, G., Gast, F., Mendoza, H., Ospina, M. & Umaña, A.M. (2004) Manual de métodos para el desarrollo de inventarios de biodiversidad. Programa de Inventarios de Biodiversidad. 236 pp. Bogotá, Instituto de Investigación de Recursos Biológicos Alexander von Humboldt.Google Scholar
Waller, P.J., Schwan, O., Ljungström, B.L., Rydzik, A. & Yeates, G.W. (2004) Evaluation of biological control of sheep parasites using Duddingtonia flagrans under commercial farming conditions on the island of Gotland, Sweden. Veterinary Parasitology 126, 299–315.Google Scholar
Yeates, G.W., Dimander, S.O., Waller, P.J. & Höglund, J. (2002) Environmental impact on soil nematodes following the use of the ivermectin sustained-release bolus or the nematophagous fungus Duddingtonia flagrans to control nematode parasites of cattle in Sweden. Acta Agriculturae Scandinavica, Section A – Animal Science 52, 233–242.CrossRefGoogle Scholar
Yeates, G.W., Dimander, S.O., Waller, P.J. & Höglund, J. (2003) Soil nematode populations beneath faecal pats from grazing cattle treated with the ivermectin sustained-release bolus or fed the nematophagous fungus Duddingtonia flagrans to control nematode parasites. Acta Agriculturae Scandinavica, Section A – Animal Science 53, 197–206.Google Scholar
Yeates, G.W., Skipp, R.A., Gray, R.A.J., Chen, L.Y. & Waghorn, T.S. (2007a) Impact on soil fauna of sheep faeces containing a range of parasite control agents. Applied Soil Ecology 35, 380–389.Google Scholar
Yeates, G.W., Skipp, R.A., Chen, L.Y., Waghorn, T.S. & Potter, J.F. (2007b) Temporal and spatial aspects of the influence on soil nematodes of depositing artificial pats of sheep faeces containing a range of parasite management agents. Applied Soil Ecology 37, 106–117.CrossRefGoogle Scholar