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Laboratory experiments to evaluate the ability of Arthrobotrys oligospora to destroy infective larvae of Cooperia species, and to investigate the effect of physical factors on the growth of the fungus

Published online by Cambridge University Press:  18 November 2009

J. Grønvold ,
H. Korsholm ,
J. Wolstrup ,
P. Nansen  and
S. A. Henriksen
J. Grønvold
Affiliation:
Royal Veterinary and Agricultural University, Institute of Hygiene and Microbiology, 13 Bülowsvej, DK-1870 Copenhagen V., Denmark
H. Korsholm
Affiliation:
Royal Veterinary and Agricultural University, Institute of Hygiene and Microbiology, 13 Bülowsvej, DK-1870 Copenhagen V., Denmark
J. Wolstrup
Affiliation:
Royal Veterinary and Agricultural University, Dept. of Microbiology and Microbial Ecology, 21 Rolighedsvej, DK-1958 Copenhagen V, Denmark
P. Nansen
Affiliation:
Royal Veterinary and Agricultural University, Institute of Hygiene and Microbiology, 13 Bülowsvej, DK-1870 Copenhagen V., Denmark
S. A. Henriksen
Affiliation:
Statc Veterinary Serum Laboratory, 27 Bülowsvej, DK-1870 Copenhagen V, Denmark
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Abstract

Laboratory investigations were designed to study the influence of temperature, pH and oxygen tension on the growth of Arthrobotrys oligospora, a nematode-trapping microfungus. Experiments were performed to evaluate the potential role of A. oligospora in destroying third-stage larvae of Cooperia spp. on agar plates and in cattle faeces. The fungus had a growth rate optimum at 23°ree;C and pII 6. Anaerobic cultivation for 23 hours at 23°ree;C and 39°ree;C inhibited fungal growth, but it did not destroy the fungus, which regained growth upon a subsequent shift to aerobic conditions at 23°ree;C. Under experimental conditions in petri-dishes containing agar, the nematode-trapping efficiency of the fungus was striking in that 100% of a population of third-stage larvae of Cooperia spp. was captured within three days of the experiment. The trapping efficiency in faeces was shown to depend upon the inoculation level. At a concentration of approximately 2500 conidia per g faeces, 99% of the larvae were destroyed. The possibilities of using nematode-trapping fungi in controlling animal-parasitic nematodes arc discussed.

Type
Research Article
Information
Journal of Helminthology , Volume 59 , Issue 2 , June 1985 , pp. 119 - 125
Copyright
Copyright © Cambridge University Press 1985

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