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Isoenzymatic pattern and structure of glutamate dehydrogenase from Ascaris suum

Published online by Cambridge University Press:  05 June 2009

C. Cutillas ,
B. Rodriguez  and
D. C. Guevara
C. Cutillas
Affiliation:
Department of Parasitology, Faculty of Pharmacy, Seville University, 41012 Seville, Spain
B. Rodriguez
Affiliation:
Department of Parasitology, Faculty of Pharmacy, Seville University, 41012 Seville, Spain
D. C. Guevara
Affiliation:
Department of Parasitology, Faculty of Pharmacy, Seville University, 41012 Seville, Spain
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Abstract

The isoenzymatic pattern of glutamate dehydrogenase (GDH) has been described for Ascaris suum, a parasite of Sus scrofa domestica. Only one band of activity has been revealed, suggesting a monomorphic condition for this enzyme. Also, the structure of GDH has been assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) and silver staining. Only one subunit was present with a molecular weight of about 55 000. A hexameric structure for GDH of A. suum is suggested.

Keywords

Ascaris suumglutamate dehydrogenaseNematodabiochemistryenzymes
Type
Research Note
Information
Journal of Helminthology , Volume 66 , Issue 4 , December 1992 , pp. 310 - 312
Copyright
Copyright © Cambridge University Press 1992

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