Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-23T22:41:12.139Z Has data issue: false hasContentIssue false
  • English
  • Français

Investigation of the Accuracy of the Clayton-Lane Faecal Egg Flotation Technique for Estimating the Numbers of Hyostrongylus rubidus (Hassall and Stiles, 1892) Eggs in Pig Faeces

Published online by Cambridge University Press:  05 June 2009

J. B. Castelino  and
I. V. Herbert
J. B. Castelino
Affiliation:
Department of Applied Zoology University College of North Wales, Bangor, Great Britain
I. V. Herbert
Affiliation:
Department of Applied Zoology University College of North Wales, Bangor, Great Britain
Get access Rights & Permissions [Opens in a new window]

Extract

We have investigated, the accuracy of a nematode egg flotation technique, using magnesium sulphate and the Clayton-Lane centrifuge system routinely used in diagnostic laboratories, to estimate the numbers of H. rubidus in porcine faeces. Eggs present in 3 g samples taken at random from a night's faecal collection from one pig were shown to be randomly distributed. The technique was not of reproducible accuracy and various sources of error were investigated.

Type
Research Article
Information
Journal of Helminthology , Volume 46 , Issue 4 , December 1972 , pp. 387 - 397
Copyright
Copyright © Cambridge University Press 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Blackman, G. E., 1942.—“Statistical and ecological studies in the distribution of species in plant communities. 1. Dispersion.” Ann. Bot. Lon. N.S., 6, 351–370.CrossRefGoogle Scholar
Brambell, M. R., 1963.—“Variation in counts of Haemonchtts contortits eggs.” J. Helminth., 37, 1–10.CrossRefGoogle ScholarPubMed
Castelino, J. B., 1967.—“An assessment of the usefulness of different faecal egg flotation and larval culture techniques for Hyostrongylus subidus. M.Sc dissertation, University of Wales.Google Scholar
Castelino, J. B., Herbert, I. V. and Lean, I. J., 1970.—“Studies on the resistance of pigs to infection with Hyostrongylus rubidus:(Hassal and Stiles, 1892). 1. Infection experiments in non-sensitised and previously sensitised growing animals.” Res. vet. Set., 12, 7–14.CrossRefGoogle Scholar
Dunn, J. E.Poteet, R. W. and Conway, D. P., 1966.—“The distribution of nematode eggs when using a dilution-egg-counting procedure.” J. Helminth., 40, 309–322.CrossRefGoogle Scholar
Herbert, I. V., Lean, I. J. and Nickson, E. W., 1969.—“Dietary factors and the production of Oesophagostomum sp. ova in breeding pigs.” Vet. Rec., 84, 569–570.CrossRefGoogle Scholar
Lean, I. J., 1966.—Helminth parasites in pigs: A survey and study of techniques. B.Sc diss. Univ. of Wales.Google Scholar
Martin, L., 1965.—“Randomness of particle distribution in human faeces and the resulting influence on helminth egg counting.” Am. J. Trop. Med. Hyg., 14, 747–759.Google Scholar
Ministry of Agriculture Fisheries and Food, 1971.—Manual of Veterinary Parasitology techniques. Tech. Bull. No. 18. H.M.S.O., London.Google Scholar
Parfitt, J. W., 1958.—“A technique for the enumeration of helminth eggs and protozoan cysts in faeces from farm animals in Britain.” Lab. pract., 7, 353–5.Google Scholar
Peters, B. G. and Leiper, J. W. G., 1940.—“The variation in dilution counts of helminth eggs.” J. Helminth., 18, 117–142.Google Scholar
Roberts, F. H. S. and O'sullivan, P. J., 1950.—“Methods for egg counts and larval cultures for strongyles infecting the gastro intestinal tract of cattle.” Aust. J. Agric. Res., 1, 99–102.Google Scholar
Teuscher, E., 1965.—“A new single method of examining faeces for the diagnosis of helminth diseases of ruminants.” Vet. Med., 12, 241–249.Google ScholarPubMed