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Infections of forest rat filaria, Breinlia booliati, in neonate and juvenile laboratory white rats

Published online by Cambridge University Press:  05 June 2009

Beng-Chuan Ho ,
Lai-Ming Chew ,
Eu-Hian Yap  and
Mulkit Singh
Beng-Chuan Ho
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 0511, Republic of Singapore
Lai-Ming Chew
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 0511, Republic of Singapore
Eu-Hian Yap
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 0511, Republic of Singapore
Mulkit Singh
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 0511, Republic of Singapore
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Abstract

The kinetics of Breinlia booliati infection in 3 inbred rat strains (Lewis, Wistar and Sprague Dawley) were investigated. One group of rats was infected as neonates (less than 24 hours of age) with third-stage larvae of B. booliati and the other group was infected as juveniles (4 weeks of age). The results showed that infection in the neonates were significantly different from the infection in the juveniles. The 60 rats infected as neonates, when necropsied between 8 to 10 months postinfection, yielded adult worms. The 2 neonatal infection groups of Lewis and Wistar strains showed highest susceptibility to the infections. The mean prepatent period was 85 days. Ninety to 95% of the infected rats were patent with microfilaraemia and a large percentage (33 to 47%) of them had high microfilaraemia counts exceeding 3000 mff/20 mm3 of blood and larger sizes (mean 157·11 mm for female adult worms and 61·88 mm for male adult worms. The adult worms were distributed equally in both the pleural (57%) and peritoneal cavity (43%). In most aspects, the neonatal infection group of the Sprague-Dawley strain was intermediate in susceptibility between the 2 neonatal infection groups of the Lewis and Wistar strains and the 3 juvenile infection groups. In contrast to neonatal infection groups, the 3 juvenile infection groups exhibited low infection rates (37%, 58% and 47% for the Lewis, Wistar and Sprague Dawley strains respectively), longer prepatent periods (mean 101 days), lower recovery rates (2 to 4%), lower adult worm loads (mean 0·4 to 0·8 female worms, and 0·2 to 0·8 male worms per rat), and smaller sizes (mean 141·24 mm for female adult worms and 53·75 mm for male adult worms). Forty-four to 57% of these infected rats harboured either single male or single female adult worms in the body cavity. Most (92%) of the adult worms recovered from the juvenile infection groups resided in the pleural cavity and the remaining 8% were recovered from the peritoneal cavity. Microfilaraemia could be detected in only 3/20 Lewis rats, 5/20 Wistar rats and 5/20 Sprague Dawley rats. The mean peak microfilaraemia of the 3 pooled juvenile infection groups was 632 mff/20 mm3 of blood, ranging from 7 mff/20 mm3 to 1856 mmf/20 mm3. Our results indicate that the susceptibility to B. booliati infection in white rats is both genetic and age-associated. The responses of the 2 distinct infection groups to B. booliati infections are discussed.

Keywords

Breinlia booliatiratinfection dynamics
Type
Research Papers
Information
Journal of Helminthology , Volume 61 , Issue 3 , September 1987 , pp. 203 - 212
Copyright
Copyright © Cambridge University Press 1987

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References

REFERENCES

Ahmed, S. S. (1967) Studies on the laboratory transmission of sub-periodic Brugia malayi and B. pahangi I. The resistance of guinea-pigs, rabbits and white mice to infection. Annals of Tropical Medicine and Parasitology, 61, 93–100.CrossRefGoogle Scholar
Beaver, P. C., Orihel, T. C. & Johnson, M. H. (1974) Dipetalonema viteae in the experimentally infected jird, Meriones unguiculatus II. Microfilaremia in relation to worm burden. Journal of Parasitology, 60, 310–315.CrossRefGoogle ScholarPubMed
Bertram, D. S. (1966) Dynamics of parasitic equilibrium in cotton rat filariasis. Advances in Parasitology, 4, 255–319.CrossRefGoogle ScholarPubMed
Chong, L. K. & Wong, M. M. (1967) Experimental infection of laboratory mice with Brugia pahangi. Medical Journal of Malaya, 21, 282.Google Scholar
Crandall, C. A., Neilson, J. T. M. & Crandall, R. B. (1982) Evaluation of inbred strains of hamsters as hosts for Brugia malayi. Transactions of the Royal Society of Tropical Medicine and Hygiene, 76, 277.CrossRefGoogle ScholarPubMed
Fox, E. G. & Schacher, J. F. (1976) A comparison of syngenic laboratory rat-strains as host for Brugia pahangi. Transactions of the Royal Society of Tropical Medicine and Hygiene, 70, 523.CrossRefGoogle Scholar
Furman, A. & Ash, L. R. (1983) Infections of Brugia pahangi in neonate mice. Journal of Parasitology, 69, 441–442.CrossRefGoogle ScholarPubMed
Ho, B. C., Singh, M., Yap, E. H. & Lim, B. L. (1976) Studies on the Malayan forest rat filaria, Breinlia booliati (Filarioidea: Onchocercidae): Transmission to laboratory rats. International Journal for Parasitology, 6, 113–116.CrossRefGoogle ScholarPubMed
Jarrett, E. E. E., Jarrett, W. F. H. & Urquhart, G. M. (1966) Immunological unresponsiveness in adult rats to the nematode Nippostrongylus brasiliensis induced by infection in early life. Nature, 211, 1310–1311.CrossRefGoogle Scholar
Kassai, T. & Aitken, I. D. (1967) Induction of immunological tolerance to Nippostrongylus brasiliensis infection. Parasitology, 57, 403–418.CrossRefGoogle ScholarPubMed
Lindsey, J. R. (1979) Historial Foundations. In: The laboratory rat. Volume 1. Biology and diseases. Editors, Baker, H. J., Lindsey, J. R. & Weisbroth, S. H. pp. 1–36. Academic Press: New York.Google Scholar
Mosier, D. E. & Johnson, B. M. (1975) Ontogeny of mouse lymphocyte function II. Development of the ability to produce antibody is modulated by T lymphocytes. Journal of Experimental Medicine, 141, 216–226.CrossRefGoogle ScholarPubMed
Mulligan, W., Urquhart, G. M., Jennings, F. W. & Neilson, J. T. M. (1965) Immunological studies on Nippostrongylus brasiliensis infection in the rat: The self-cure phenomenon. Experimental Parasitology, 16, 341–347.CrossRefGoogle ScholarPubMed
Nielson, J. P. (1978) Primary infections of Dipetalonema viteae in an outbred and five inbred strains of golden hamsters. Journal of Parasitology, 64, 378–380.CrossRefGoogle Scholar
Ogilvie, B. M. (1965) Role of adult worms in immunity of rats to Nippostrongylus brasiliensis. Parasitology, 55, 325–335.CrossRefGoogle ScholarPubMed
Olson, L. J. (1959) The survival of migratory and post-migratory stages of Litomosoides carinii in white rats. Journal of Parasitology, 45, 182–188.CrossRefGoogle ScholarPubMed
Pilarski, L. M. (1977) Ontogeny of cell-mediated immunity I. Early development of alloantigen-specific cytotoxic T-cell precursors in postnatal mice. Journal of Experimental Medicine, 146, 887–892.CrossRefGoogle ScholarPubMed
Singh, M., Yap, E. H., Ho, B. C., Kang, K. L., Lim, P. C. & Lim, B. L. (1976) Studies on the Malayan forest rat filaria. Breinlia booliati (Filarioidea: Onchocercidae): Course of development in rat host. Journal of Helminthology, 50, 103–110.CrossRefGoogle ScholarPubMed
Soloman, J. B. (1971) Foetal and Neonatal Immunology. pp. 1–381. North-Holland Publishing Company.Google Scholar
Woodruff, M. F. A & Simpson, L. O. (1955) Induction of tolerance to skin homografts in rats by injection of cells from the prospective donor soon after birth. British Journal of Experimental Pathology, 36, 494–499.Google ScholarPubMed