Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-27T22:54:08.280Z Has data issue: false hasContentIssue false

In vitro transformation of Diplostomum spathaceum (Digenea) cercariae and short term maintenance of post-penetration larvae in vitro

Published online by Cambridge University Press:  05 June 2009

S. K. Whyte
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB9 2TN
L. H. Chappell
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB9 2TN
C. J. Secombes
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB9 2TN

Abstract

Optimum conditions for the in vitro transformation of Diplostomum spathaceum cercariae have been established by which large numbers of post-penetration larvae (diplostomules) can be produced; short-term maintenance of diplostomules in vitro is described. Experimental studies on this migratory larval stage may now be possible.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bell, E. J. & Smyth, J. D. (1958) Cytological and histochemical criteria for evaluating development of trematodes and pseudophyllidean cestodes in vivo and in vitro. Parasitology, 48, 131148.CrossRefGoogle ScholarPubMed
Betterton, C. (1974) Studies on the host specificity of the eyefluke, Diplostomum spathaceum, in brown and rainbow trout. Parasitology, 69, 1129.CrossRefGoogle Scholar
Brink, L. H., Mclaren, D. J. & Smithers, S. R. (1977) Schistosoma mansoni: A comparative study of artificially transformed schistosomula and schistosomula recovered after cercarial penetration of isolated skin. Parasitology, 74, 7386.CrossRefGoogle ScholarPubMed
Butterworth, A. E. (1984) Cell mediated damage to helminths. In: Advances in Parasitology 23, (editors, Baker, J. R., & Muller, R.) pp 144207. Academic Press: London.Google Scholar
Clegg, J. A. & Smithers, S. R. (1972) The effects of the immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivationin vitro. International Journal for Parasitology, 2, 7998.CrossRefGoogle Scholar
Cousin, C. E., Stirewalt, M. A., Dorsey, C. H. & Watson, L. P. (1986) Schistosoma mansoni: Comparative development of schistosomules produced by artificial techniques. Journal of Parasitology, 72, 606609.CrossRefGoogle ScholarPubMed
Erasmus, D. A. (1959) The migration of Cercaria X Baylis (Strigeida) within the intermediate host. Parasitology, 49, 173190.CrossRefGoogle ScholarPubMed
Eveland, L. K. (1972) Schistosoma mansoni: Conversion of cercariae to schistosomula. Experimental Parasitology, 32, 261264.CrossRefGoogle ScholarPubMed
Ferguson, M. S. (1943) Migration and localisation of an animal parasite within the host. Journal of Experimental Parasitology, 93, 375403.Google Scholar
Gilbert, B., Darosa, M., Borojevic, R. & Pellegrino, J. (1972) Schistosoma mansoni: in vitro transformation of cercariae into schistosomula. Parasitology, 64, 333339.CrossRefGoogle ScholarPubMed
Howells, R. E., Ramalho-Pinto, F. J., Gazzinelli, G., Deoliveira, C. C., Figueiredo, E. A. & Pellegrino, J. (1974) Schistosoma mansoni: Mechanism of tail loss and its significance to host penetration. Experimental Parasitology, 36, 373385.CrossRefGoogle ScholarPubMed
Kannangara, D. W. W. & Smyth, J. D. (1974) In vitro cultivation of Diplostomum spathaceum and Diplostomum phoxini metacercariae. International Journal for Parasitology, 4, 667673.CrossRefGoogle ScholarPubMed
Kassis, A. I., Aikawa, M. & Mahmoud, A. F. (1979) Mouse antibody-dependent eosinophil and macrophage adherence and damage to schistosomula ofSchistosoma mansoni. Journal of Immunology, 122, 398405.CrossRefGoogle Scholar
Leno, G. H. & Holloway, H. L. Jr. (1986) The culture of Diplostomum spathaceum metacercariae on the chick chorioallantois. Journal of Parasitology, 72, 555558.CrossRefGoogle ScholarPubMed
Mitchell, J. S., Halton, D. W. & Smyth, J. D. (1978) Observations on the in vitro culture of Cotylurus erraticus (Trematoda: Strigeidae). International Journal for Parasitology, 8, 389397.CrossRefGoogle Scholar
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A., Figueiredo, E. A. & Pellegrino, J. (1974) Schistosoma mansoni: defined system for stepwise transformation of cercariae to schistosomulain vitro. Experimental Parasitology, 36, 360372.CrossRefGoogle ScholarPubMed
Ratanarat-Brockelman, C. (1974) Migration of Diplostomum spathaceum in the fish intermediate host. Zeitschrift für Parisitenkunde, 43, 123134.CrossRefGoogle Scholar
Shariff, M., Richards, R. H. & Sommerville, C. (1980) The histopathology of acute and chronic infections of rainbow trout Salmo gairdneri Richardson with eyefluke, Diplostomum spp. Journal of Fish Diseases, 3, 455465.CrossRefGoogle Scholar
Stables, J. N. & Chappell, L. H. (1986) Diplostomum spathaceum (Rud. 1819): effects of physical factors on the infection of rainbow trout (Salmo gairdneri) by cercariae. Parasitology, 93, 7179.CrossRefGoogle ScholarPubMed
Stirewalt, M. A. & Uy, A. (1969) Schistosoma mansoni: cercarial penetration and schistosomule collection in an in vitro system. Experimental Parasitology, 26, 1728.CrossRefGoogle Scholar
Tavares, C. A. P., Soares, R. C., Coelho, P. M. Z. & Gazzinelli, G. (1978) Schistosoma mansoni: Evidence for a role of serum factors in protecting artificially transformed schistosomula against antibody-mediated killing in vitro. Parasitology, 77, 225233.CrossRefGoogle ScholarPubMed
Ulmer, M. J. (1970) Notes on rearing snails in the laboratory. In: Experiments and Techniques in Parasitology (editors Macinnes, A.J, & Voge, M.) Published W. H. Freeman, San Francisco pp. 143144.Google Scholar
Voge, M. & Jeong, K. (1971) Growth in vitro of Cotylurus lutzi Basch 1969 (Trematoda: Strigeidae), from tetracotyle to patent adult. International Journal for Parasitology, 1, 139143.CrossRefGoogle ScholarPubMed
Whyte, S. K., Allan, J. C., Secombes, C. J. & Chappell, L. H. (1987) Cercariae and diplostomules of Diplostomum spathaceum (Digenea) elicit an immune response in rainbow trout, Salmo gairdneri Richardson. Journal of Fish Biology, 31 (Supplement A), 185190.CrossRefGoogle Scholar