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In vitro nematicidal activity of two ferrocenyl chalcones against larvae of Haemonchus contortus (L3) and Nacobbus aberrans (J2)

Published online by Cambridge University Press:  11 September 2020

J. Vázquez-Bravo Open the ORCID record for J. Vázquez-Bravo [Opens in a new window] ,
L. Aguilar-Marcelino Open the ORCID record for L. Aguilar-Marcelino [Opens in a new window] ,
G.S. Castañeda-Ramírez Open the ORCID record for G.S. Castañeda-Ramírez [Opens in a new window] ,
I. De los Santos-Pérez Open the ORCID record for I. De los Santos-Pérez [Opens in a new window] ,
R.E. Arroyo-Carmona Open the ORCID record for R.E. Arroyo-Carmona [Opens in a new window] ,
S. Bernès Open the ORCID record for S. Bernès [Opens in a new window] ,
U. Hernández-Pareja Open the ORCID record for U. Hernández-Pareja [Opens in a new window] ,
O. Gómez-Rodríguez Open the ORCID record for O. Gómez-Rodríguez [Opens in a new window]  and
G.H. Rosas-Saito Open the ORCID record for G.H. Rosas-Saito [Opens in a new window]
J. Vázquez-Bravo
Affiliation:
Colaborador del Laboratorio de Nuevos Materiales de la Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Avenida San Claudio y 18 Sur, Colonia Jardínes de San Manuel, CP 72570, Puebla, Puebla, Mexico
L. Aguilar-Marcelino*
Affiliation:
Laboratorio de Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Carretera Federal Cuernavaca-Cuautla no. 8534, Colonia Progreso, CP 62550, Jiutepec, Morelos, Mexico
G.S. Castañeda-Ramírez
Affiliation:
Laboratorio de Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Carretera Federal Cuernavaca-Cuautla no. 8534, Colonia Progreso, CP 62550, Jiutepec, Morelos, Mexico
I. De los Santos-Pérez
Affiliation:
Colaborador del Laboratorio de Nuevos Materiales de la Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Avenida San Claudio y 18 Sur, Colonia Jardínes de San Manuel, CP 72570, Puebla, Puebla, Mexico
R.E. Arroyo-Carmona
Affiliation:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Avenida San Claudio y 18 Sur, Colonia Jardínes de San Manuel, CP 72570, Puebla, Puebla, Mexico
S. Bernès
Affiliation:
Instituto de Física, Benemérita Universidad Autónoma de Puebla, Avenida San Claudio y 18 Sur, Colonia Jardínes de San Manuel, CP 72570, Puebla, Puebla, Mexico
U. Hernández-Pareja
Affiliation:
Centro de Química, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio IC8 Complejo de Ciencias, Avenida San Claudio y 18 Sur, Colonia Jardínes de San Manuel, CP 72570, Puebla, Puebla, Mexico
O. Gómez-Rodríguez
Affiliation:
Colegio de Postgraduados, Carretera México-Texcoco, Km 36.5, Montecillo, Texcoco, Estado de México, Mexico
G.H. Rosas-Saito
Affiliation:
INECOL, AC, Red de Estudios Moleculares Avanzados, Unidad de Microscopía Avanzada del Clúster Científico y Tecnológico BioMimic, Carretera Antigua a Coatepec no. 351, Colonia El Haya, CP 91070, Xalapa, Veracruz, Mexico
*
Author for correspondence: L. Aguilar-Marcelino, E-mail: [email protected]
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Abstract

The main goal of this work was to evaluate the in vitro biological activity of two ferrocenyl chalcones (FcC-1 and FcC-2) against Haemonchus contortus (third-stage larvae (L3)) and Nacobbus aberrans (second-stage juveniles (J2)). Both compounds were synthesized and characterized by usual spectroscopic methods and their molecular structures were confirmed by single-crystal X-ray diffractometry. Nematode strains were examined in terms of percentage mortality of H. contortus (L3) by the action of FcC-1, which showed an effectivity of 100% at a concentration of 342 μM in 24 h, with EC50 = 20.33 μM and EC90 = 162.76 μM, whereas FcC-2 had an effectivity of 72% at a concentration of 342 μM in 24 h, with EC50 = 167.39 μM and EC90 = 316.21 μM. The effect of FcC-1 against nematode phytoparasite N. aberrans showed a better percentage of 95% at a concentration of 342 μM, with EC50 = 7.18 μM and EC90 = 79.25 μM, whereas the effect of FcC-2 was 87% at 342 μM, with EC50 = 168 μM and EC90 = 319.56 μM at 36 h. After treatment, the scanning electron micrographs revealed deformities in the dorsal flank and posterior part close to the tail of H. contortus L3. They showed moderate in vitro nematicidal activity against H. contortus L3 and N. aberrans J2.

Keywords

Ferrocenyl chalconesnematocidal activityHaemonchus contortusNacobbus aberrans
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e190
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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