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Immunological analysis of Opisthorchis and Clonorchis antigens

Published online by Cambridge University Press:  05 June 2009

S. Sirisinha ,
D. Sahassananda ,
D. Bunnag  and
H. J. Rim
S. Sirisinha
Affiliation:
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand
D. Sahassananda
Affiliation:
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand
D. Bunnag
Affiliation:
Hospital for Tropical Diseases, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
H. J. Rim
Affiliation:
Department of Parasitology, College of Medicine, Korea University, Seoul, Korea
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Abstract

Immunoreactive components of Opisthorchis viverrini and Clonorchis sinensis were analysed by enzyme-linked immunosorbent assay (ELISA), radioimmunoprecipitation and immunoblotting. Somatic extracts from these two liver flukes as well as from other related parasites, together with the metabolic products, were tested for their reactivities with sera from patients with opisthorchiasis and clonorchiasis. A significant cross-reactivity in the ELISA was noted between Opisthorchis and Clonorchis. Immunoblotting and radioimmunoprecipitation analyses showed that the 89-kD protein which was previously shown to be a predominant metabolic product of O. viverrini reacted with sera from both groups of patients. However, an antigen with a molecular weight of 16 kD, apparently a predominant somatic component, appeared to be specific for O. viverrini.

Keywords

Opisthorchis viverriniClonorchis sinensisELISAimmunoprecipitationimmunoblotting
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 2 , June 1990 , pp. 133 - 138
Copyright
Copyright © Cambridge University Press 1990

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References

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