Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-23T22:04:05.611Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunization with crude antigens plus aluminium hydroxide protects cattle from Fasciola hepatica infection

Published online by Cambridge University Press:  03 March 2011

L. Guasconi ,
M.C. Serradell ,
J. Borgonovo ,
A.P. Garro ,
H. Varengo ,
G. Caffe  and
D.T. Masih
L. Guasconi
Affiliation:
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba, Argentina
M.C. Serradell
Affiliation:
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba, Argentina
J. Borgonovo
Affiliation:
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba, Argentina
A.P. Garro
Affiliation:
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba, Argentina
H. Varengo
Affiliation:
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba, Argentina
G. Caffe
Affiliation:
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba, Argentina
D.T. Masih*
Affiliation:
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba, Argentina
*
*Fax: +54-0351-4333048 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The ability of total homogenate (TH) of Fasciola hepatica conjugated with aluminium hydroxide (alum) or Freund's complete adjuvant (FCA) to protect cattle against experimental fasciolosis was evaluated. Compared with the infected group, the immunized animals with alum-TH and FCA-TH presented a significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher percentage of short-sized worms, a marked reduction in the released eggs in faeces (89% and 57%, respectively), as well as an increased production of specific antibodies before infection. The alum-TH immunized group also showed a significant increase in the antigen-specific proliferation of peripheral blood mononuclear cells (PBMC) as early as 4 weeks before infection. Although both immunized groups (alum-TH and FCA-TH) were able to develop an efficient protective immune response to metacercarial challenge, an earlier PBMC response, lower hepatic damage and less effect on weight gain were found in alum-immunized animals. Therefore, alum is a good candidate for future immunization against bovine fasciolosis.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 1 , March 2012 , pp. 64 - 69
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barnes, E.H., Dobson, R.J. & Barger, I.A. (1995) Worm control and anthelminthic resistance: adventures with a model. Parasitology Today 11, 56–63.CrossRefGoogle Scholar
Cervi, L., Rubinstein, H.R. & Masih, D.T. (1992) Serological, electrophoretic and biological properties of Fasciola hepatica antigens. Revista do Instituto de Medicina de São Paulo 34, 517–525.Google ScholarPubMed
Claassen, E., de Leeuw, W., de Greeve, P., Hendriksen, C. & Boersma, W. (1992) Freund's complete adjuvant: an effective but disagreeable formula. Research in Immunology 143, 478–483.Google Scholar
Dalton, J.P. & Mulcahy, G. (2001) Parasite vaccines – a reality? Veterinary Parasitology 98, 149–167.Google Scholar
Dalton, J.P., McGonigle, S., Rolph, T.P. & Andrews, S.J. (1996) Induction of protective immunity in cattle against infection with Fasciola hepatica by vaccination with cathepsin L proteinases and with hemoglobin. Infection and Immunity 64, 5066–5074.CrossRefGoogle ScholarPubMed
Hillyer, G.V., Haroun, E.M., Hernandez, A. & De Galanes, M.S. (1987) Acquired resistance to Fasciola hepatica in cattle using a purified adult worm antigen. American Journal of Tropical Medicine and Hygiene 37, 363–369.CrossRefGoogle ScholarPubMed
Kleinman, N.R., Kier, A.B., Diaconu, E. & Lass, J.H. (1993) Posterior paresis induced by Freund's adjuvant in guinea pigs. Laboratory Animal Science 43, 364–366.Google ScholarPubMed
Knox, D.P., Redmond, D.L., Sauce, P.J. & Newlands, G.F.J. (2001) The contribution of molecular biology to the development of vaccines against nematode and trematode parasites of domestic rumiants. Veterinary Parasitology 101, 311–335.CrossRefGoogle Scholar
Lowry, O.H., Roserbrough, H.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Mas-Coma, S., Angles, R., Esteban, J.G., Bargues, M.D., Buchon, P., Franjen, M. & Strauss, W. (1999) The Northern Bolivian Altiplano: a region highly endemic for human fasciolosis. Tropical Medicine & International Health 4, 454–467.CrossRefGoogle Scholar
Mas-Coma, S., Barques, M.D. & Valero, M.A. (2005) Fascioliasis and other plant-borne trematode zoonoses. International Journal for Parasitology 35, 1255–1278.Google Scholar
McGonigle, S. & Dalton, J.P. (1995) Isolation of Fasciola hepatica haemoglobin. Parasitology 111, 209–215.CrossRefGoogle ScholarPubMed
McManus, D.P. & Dalton, J.P. (2006) Vaccines against the zoonotic trematodes Schistosoma japonicum, Fasciola hepatica and Fasciola gigantica. Parasitology 133, S43–S61.CrossRefGoogle ScholarPubMed
Morrison, C.A., Colin, T., Sexton, J.L., Bowen, F., Wicker, J., Friedel, T. & Spithill, T.W. (1996) Protection of cattle against Fasciola hepatica infection by vaccination with glutathione S-transferase. Vaccine 4, 1603–1612.CrossRefGoogle Scholar
Mulcahy, G., Joyce, P. & Dalton, J.P. (1999) Immunology of Fasciola hepatica infection. pp. 341–376in Dalton, J.P. (Ed.) Fasciolosis. Wallingford, UK, CAB International.Google Scholar
Mulcahy, G., O'Neill, S., Donnelly, S. & Dalton, J.P. (2004) Helminths at mucosal barriers—interaction with the immune system. Advanced Drug Delivery Reviews 56, 853–868.CrossRefGoogle ScholarPubMed
O'Hagan, D.T., MacKichan, M.L. & Singh, M. (2001) Recent developments in adjuvants for vaccines against infectious diseases. Biomolecular Engineering 18, 69–85.Google Scholar
Reszka, N., Cornelissen, J.B.W.J., Harmsen, M.M., Biénkowska-Szewczyk, K., de Bree, J., Boersma, W.J. & Rijsewijk, F.A.M. (2005) Fasciola hepatica procathepsin L3 protein expressed by a baculovirus recombinant can partly protect rats against fasciolosis. Vaccine 23, 2987–2993.CrossRefGoogle ScholarPubMed
Roepstorff, A. & Nansen, P. (1998) The epidemiology, diagnosis and control of helminth parasites of swine. FAO Animal Health Manual 3. Rome, Italy, Food and Agriculture Organization of the United Nations.Google Scholar
Spithill, T.W. & Dalton, J.P. (1998) Progress in development of liver fluke vaccines. Parasitology Today 14, 224–228.CrossRefGoogle ScholarPubMed
van Milligen, F.J., Cornelissen, J.B.W.J. & Bokhout, B.A. (2000) Fasciola hepatica: An antigen fraction derived from newly excysted juveniles, containing an immunoreactive 32-kDa protein, induces strong protective immunity in rats. Exerimental Parasitology 94, 163–171.CrossRefGoogle ScholarPubMed
Villa-Mancera, A., Quiroz Romero, H., Correa, D., Ibarra, F., Reyes Pérez, M., Reyes Vivas, H., López Velázquez, G., Gazarian, K., Gazarian, T. & Alonso, R.A. (2008) Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library. Parasitology 135, 1437–1445.Google Scholar