Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-15T23:21:39.676Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunization against experimental rabbit cysticercosis using liposome-associated antigen preparations

Published online by Cambridge University Press:  05 June 2009

P. S. Craig  and
O. Zumbuehl
P. S. Craig
Affiliation:
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, England
O. Zumbuehl
Affiliation:
Physikalische Pharmazie-ETH, Sonneggstrasse 5, CH–8092 Zurich, Switzerland
Get access Rights & Permissions [Opens in a new window]

Abstract

Rabbits were vaccinated once, by subcutaneous and intradermal injection, with sonicates of oncospheres (TpO) or conditioned media from in vitro maintained mature metacestodes (TpMcES) of Taenia pisiformis. Extracts were either incorporated into or mixed with unilammelar liposomes (reverse phase evaporative vesicles) or emulsified in Freund's Incomplete Adjuvant (FIA). Control groups received liposomes or FIA without antigen, or antigen preparation without adjuvant. Rabbits were challenged orally two weeks after vaccination with approximately 1500 eggs of T. pisiformis and necropsied eight weeks after challenge. A mean of 155 cysts was recovered from seven control rabbits. A 67% reduction in peritoneal cyst numbers was obtained in TpO–IFA vaccinated rabbits compared to 75% for the TpO–liposome entrapped group. The highest level of protection (86%) was obtained when TpO was mixed with but not entrapped in liposomes. Only 32% and 39% reduction in peritoneal cyst numbers was obtained after immunizing with the TpMcES preparation in liposomes or IFA respectively, however >85% of peritoneal metacestodes were dead (necrotic or calcified) and suggests a different immune response than occurs after vaccination with oncosphere extracts. Specific anti-oncospheral or anti-metacestode ES antibody (IgG) responses at two weeks post vaccination were similar in rabbits immunized with liposome or IFA associated extracts.

Keywords

Taenia pisiformisimmunizationoncospheremetacestodeliposomesrabbits
Type
Research Papers
Information
Journal of Helminthology , Volume 62 , Issue 1 , March 1988 , pp. 58 - 62
Copyright
Copyright © Cambridge University Press 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bangham, A. D., Standish, M. M. & Watkins, J. C. (1965) Diffusion of univalent ions across the lamellae of swollen phospholipids. Journal of Molecular Biology, 13, 238–252.CrossRefGoogle ScholarPubMed
Craig, P. S. (1984) Circulating antigens, antibodies and immune complexes in experimental Taenia pisiformis infections of rabbits. Parasitology, 89, 121–131.Google Scholar
Craig, P. S. & Rickard, M. D. (1981) Anti-oncospheral antibodies in the serum of lambs experimentally infected with either Taenia ovis or Taenia hydatigena. Zeitschrift für Parasitenkunde, 64, 169–177.Google Scholar
Croft, S. L. (1986) Liposomes in the treatment of parasitic diseases. Pharmacy International, 7, 229–233.Google Scholar
Flisser, A., Perez-Montfort, R. & Larralde, C. (1979) The immunology of human and animal cysticercosis: a review. Bulletin of the World Health Organization, 57, 839–856.Google ScholarPubMed
Gemmell, M. A. & Soulsby, E. J. L. (1968) The development of acquired immunity to tapeworms and progress towards active immunization, with special reference to Echinococcus spp. Bulletin of the World Health Organization, 39, 45–55.Google Scholar
Rajasekariah, G. R., Rickard, M. D. & Mitchell, G. F. (1980) Density gradient separation of Taenia pisiformis oncospheres. Journal of Parasitology, 66, 355–356.Google Scholar
Rajasekariah, G. R., Rickard, M. D. & O'donnell, I. J. (1985) Taenia pisiformis: protective immunization of rabbits with solubilized oncospheral antigen. Experimental Parasitology, 59, 321–327.Google Scholar
Rickard, M. D. (1982) Immunization against infection with larval taeniid cestodes using oncospheral antigens. In: Cysticercosis: Present State of Knowledge and Perspectives (editors Flisser, A., Willms, K., Laclette, J. P., Larralde, C., Ridaura, C. & Beltran, F.): Academic Press: London, pp. 633–646.Google Scholar
Rickard, M. D. & Williams, J. F. (1982) Hydatidosis/cysticercosis: immune mechanisms and immunization against infection. Advances in Parasitology, 21, 229–296.CrossRefGoogle ScholarPubMed
Siddiqui, W. A., Kan, S. C., Kramer, K., Case, S. & Palmer, K. (1981) Use of a synthetic adjuvant in an effective vaccination of monkeys against malaria. Nature, 289, 64–66.Google Scholar
Szoka, F. & Papahadjopoulos, D. (1978) Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse phase evaporation. Proceedings of the National Academy of Science, USA, 75, 4194–4198.Google Scholar
Tom, B. H. (1980) An overview: liposomes and immunobiology—macrophages, liposomes and tailored immunity. In: Liposomes and Immunobiology (editors Tom, B. H. & Six, H. R.): Elsevier North Holland: Amsterdam, pp. 3–22.Google Scholar
Van Rooijen, N. & Van Nieuwmegen, R. (1980) Liposomes in immunology: evidence that their adjuvant effect results from surface exposition of the antigens. Cellular Immunology, 49, 402–407.Google Scholar