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Immune responses to polyethylenimine-mannose-delivered plasmid DNA encoding a Fasciola gigantica fatty acid binding protein in mice

Published online by Cambridge University Press:  02 September 2009

S. Smitha ,
O.K. Raina ,
B.P. Singh ,
S. Samanta ,
R. Velusamy ,
S. Dangoudoubiyam ,
A. Tripathi ,
P.K. Gupta ,
Bhaskar Sharma  and
Meeta Saxena
S. Smitha
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, UP, India
O.K. Raina*
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, UP, India
B.P. Singh
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, UP, India
S. Samanta
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, UP, India
R. Velusamy
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, UP, India
S. Dangoudoubiyam
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, UP, India
A. Tripathi
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, UP, India
P.K. Gupta
Affiliation:
Division of Animal Biotechnology, IVRI, Izatnagar, UP, India
Bhaskar Sharma
Affiliation:
Division of Animal Biochemistry, IVRI, Izatnagar, UP, India
Meeta Saxena
Affiliation:
Division of Animal Biochemistry, IVRI, Izatnagar, UP, India
*
*E-mail: [email protected]
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Abstract

Fasciola gigantica fatty acid binding protein (FABP) was evaluated for evoking an immune response in mice, by delivering the gene coding for this protein with mannosylated-polyethylenimine (PEI) to peritoneal cells. Mice were immunized with 50 μg recombinant plasmid DNA (Group I) or DNA-PEI-mannose (a 22 kDa linear cationic polymer with mannose ligand) (Group II) via the intraperitoneal route. Antibody studies showed no significant humoral immune response evoked to this DNA immunization with either PEI-mannose-delivered or naked DNA. However, on protein boosting of these DNA-primed mice there was a significant enhancement of antibody titre. Flow cytometric bead array was used to measure quantitites of interleukin (IL)-2, IL-4, IL-5, interferon-γ (IFN-γ) and tumour necrosis factor (TNF) cytokines. Overexpression of T-helper 1 (Th1) cytokines such as IFN-γ and TNF, with a lower but significant expression of the T-helper 2 (Th2) cytokine IL-5 was detected. Gene delivery using polyethylenimine-mannose ligand showed significant expression of IFN-γ and TNF (P < 0.05), but no significant difference in IL-2, IL-4 and IL-5 (P>0.05) cytokine expression was observed between naked-DNA- and mannosylated PEI-DNA-delivered mice. Naked- or PEI-delivered-DNA immunization produced insignificant levels of IL-2 and IL-4 (P>0.05) cytokines in both groups of mice.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 2 , June 2010 , pp. 149 - 155
Copyright
Copyright © Cambridge University Press 2009

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