Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-09T15:10:08.287Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification and characterization of immunodominant linear epitopes on the antigenic region of a serine protease in newborn Trichinella larvae

Published online by Cambridge University Press:  20 May 2015

Y. Yang ,
I. Vallée ,
S.A. Lacour ,
P. Boireau ,
S.P. Cheng  and
M.Y. Liu
Y. Yang*
Affiliation:
Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun130062, PR China JRU BIPAR, Animal Health Laboratory, ANSES, Maisons-Alfort, France State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, PR China
I. Vallée
Affiliation:
JRU BIPAR, Animal Health Laboratory, ANSES, Maisons-Alfort, France
S.A. Lacour
Affiliation:
JRU BIPAR, Animal Health Laboratory, ANSES, Maisons-Alfort, France
P. Boireau
Affiliation:
Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun130062, PR China JRU BIPAR, Animal Health Laboratory, ANSES, Maisons-Alfort, France
S.P. Cheng*
Affiliation:
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, PR China
M.Y. Liu*
Affiliation:
Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun130062, PR China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yang zhou, 225009, PR China
*
*E-mail: [email protected], [email protected] and [email protected]
*E-mail: [email protected], [email protected] and [email protected]
*E-mail: [email protected], [email protected] and [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

An immunodominant serine protease of Trichinella spiralis named NBL1 showed encouraging potential in early diagnosis of trichinellosis in pigs and elicited protective immune responses during infection of animals. To further define serological reagents for diagnostic use, the specific epitopes on NBL protein recognized by the antibody responses of different susceptible hosts need to be defined. The present study described comprehensive mapping of immunodominant linear epitopes in the antigenic region (NBL-C, the C-terminal part of the protein) using various serum samples obtained from three kinds of hosts – pig, wild boar and mice. We identified six peptides which were commonly recognized by sera from pigs experimentally infected with Trichinella and pigs immunized with rNBL1-C; five and four peptides were recognized by sera from wild boars and mice infected with Trichinella, respectively. Three peptides (NBL1-6, -7 and -9) were commonly recognized by antisera in all three hosts, which share the sequence PSSGSRPTYP. We also found that one peptide (NBL1-12) was only recognized by antibodies from pigs immunized with rNBL1-C. The identification of specific epitopes targeted by the host antibody response is important both for understanding the natural response to infection and for the development of subunit vaccines and diagnostic tools for trichinellosis.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 2 , March 2016 , pp. 232 - 237
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Boireau, P., Liu, M., Fu, B., Le Rhun, D., Bahuon, C., Vallee, I., Le Guerhier, F., Hernandez, R.B. & Wu, X. (2007) Polypeptides Recognized by Anti-Trichinella Antibodies, and uses Thereof. WO Patent 2,007,090,960.Google Scholar
Bungiro, R.D. Jr, Sun, T., Harrison, L.M., Shoemaker, C.B. & Cappello, M. (2008) Mucosal antibody responses in experimental hookworm infection. Parasite Immunology 30, 293–303.CrossRefGoogle ScholarPubMed
Buscaglia, C.A., Campetella, O., Leguizamón, M.S. & Frasch, A.C.C. (1998) The repetitive domain of Trypanosoma cruzi trans-sialidase enhances the immune response against the catalytic domain. Journal of Infectious Diseases 177, 431–436.CrossRefGoogle ScholarPubMed
Cho-Ngwa, F., Liu, J. & Lustigman, S. (2010) The Onchocerca volvulus cysteine proteinase inhibitor, Ov-CPI-2, is a target of protective antibody response that increases with age. PLoS Neglected Tropical Diseases 4, e800.CrossRefGoogle ScholarPubMed
Gottstein, B., Pozio, E. & Nöckler, K. (2009) Epidemiology, diagnosis, treatment, and control of trichinellosis. Clinical Microbiology Reviews 22, 127–145.CrossRefGoogle ScholarPubMed
Gruber, A. & Zingales, B. (1993) Trypanosoma cruzi: characterization of two recombinant antigens with potential application in the diagnosis of Chagas’ disease. Experimental Parasitology 76, 1–12.CrossRefGoogle ScholarPubMed
Kemp, D., Coppel, R. & Anders, R. (1987) Repetitive proteins and genes of malaria. Annual Reviews in Microbiology 41, 181–208.CrossRefGoogle ScholarPubMed
Leguizamón, M.S., Campetella, O.E., González Cappa, S. & Frasch, A. (1994) Mice infected with Trypanosoma cruzi produce antibodies against the enzymatic domain of trans-sialidase that inhibit its activity. Infection and Immunity 62, 3441–3446.CrossRefGoogle ScholarPubMed
Liu, M., Wang, X., Fu, B., Li, C., Wu, X., Le Rhun, D., Chen, Q. & Boireau, P. (2007) Identification of stage-specifically expressed genes of Trichinella spiralis by suppression subtractive hybridization. Parasitology 134, 1443–1455.CrossRefGoogle ScholarPubMed
McCoy, K.D., Stoel, M., Stettler, R., Merky, P., Fink, K., Senn, B.M., Schaer, C., Massacand, J., Odermatt, B. & Oettgen, H.C. (2008) Polyclonal and specific antibodies mediate protective immunity against enteric helminth infection. Cell Host & Microbe 4, 362–373.CrossRefGoogle ScholarPubMed
Parodi, A.J., Pollevick, G.D., Mautner, M., Buschiazzo, A., Sanchez, D.O. & Frasch, A. (1992) Identification of the gene(s) coding for the trans-sialidase of Trypanosoma cruzi . The EMBO Journal 11, 1705–1715.CrossRefGoogle ScholarPubMed
Wei, J., Gu, Y., Yang, J., Yang, Y., Wang, S., Cui, S. & Zhu, X. (2011) Identification and characterization of protective epitope of Trichinella spiralis paramyosin. Vaccine 12, 3162–3168.CrossRefGoogle Scholar