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Fungus–benzimidazole interactions: a prerequisite to deploying egg-parasitic fungi Paecilomyces lilacinus and Verticillium chlamydosporium as biocontrol agents against fascioliasis and amphistomiasis in ruminant livestock

Published online by Cambridge University Press:  24 August 2009

R.K. Singh ,
P.K. Sanyal ,
N.K. Patel ,
A.K. Sarkar ,
A.K. Santra ,
S. Pal  and
S.C. Mandal
R.K. Singh
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg491 001, Chhattisgarh, India
P.K. Sanyal*
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg491 001, Chhattisgarh, India
N.K. Patel
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg491 001, Chhattisgarh, India
A.K. Sarkar
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg491 001, Chhattisgarh, India
A.K. Santra
Affiliation:
Department of Livestock Production and Management, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg491 001, Chhattisgarh, India
S. Pal
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg491 001, Chhattisgarh, India
S.C. Mandal
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg491 001, Chhattisgarh, India
*
*Fax: +91 788 2252139 E-mail: [email protected]
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Abstract

In vitro trials investigating the effects of albendazole and triclabendazole anthelmintics on the growth profiles of the egg-parasitic fungi Paecilomyces lilacinus and Verticillium chlamydosporium were undertaken. In addition, in vivo trials were conducted in goats fed on millet grain cultures of each fungus and administered albendazole and triclabendazole anthelmintics. In vitro growth revealed V. chlamydosporium to be more sensitive to albendazole compared to P. lilacinus. In contrast, triclabendazole had the least inhibitory effect on in vitro growth of both P. lilacinus and V. chlamydosporium. Similar to albendazole, growth of P. lilacinus was more vigorous at 0.5 ppm concentration of triclabendazole. Efforts to re-isolate these egg-parasitic fungi from faeces of goats fed on fungal millet grain cultures before and following single intraruminal administration of albendazole and triclabendazole showed that P. lilacinus was not able to be re-isolated from the faeces at any sampling period. In contrast, V. chlamydosporium was able to be re-isolated from the faeces at all of the sampling periods except for the samples taken at 8–18 h and 18–24 h after administration of albendazole and triclabendazole, respectively. Lack of fungal activity at these times coincided with peak plasma availability of anthelmintics and suggests faecal levels of drugs were also high at these times and impacted negatively on fungal viability.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 2 , June 2010 , pp. 123 - 131
Copyright
Copyright © Cambridge University Press 2009

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References

De, S., Sanyal, P.K., Sarkar, A.K., Patel, N.K., Pal, S. & Mandal, S.C. (2008) Screening for Indian isolates of egg parasitic fungi for use in biological control of fascioliasis and amphistomiasis in ruminant livestock. Journal of Helminthology 82, 271–277.CrossRefGoogle ScholarPubMed
Hennessy, D.R., Steel, J.W., Lacey, E., Eagleson, G.K. & Prichard, R.K. (1989) The disposition of albendazole in sheep. Journal of Veterinary Pharmacology and Therapeutics 12, 421–429.CrossRefGoogle ScholarPubMed
Hennessy, D.R., Steel, J.W. & Prichard, R.K. (1993) Biliary secretion and enterohepatic recycling of fenbendazole metabolites in sheep. Journal of Veterinary Pharmacology and Therapeutics 16, 132–140.CrossRefGoogle ScholarPubMed
Hennessy, D.R., Ali, D.N. & Tremain, S.A. (1994) The partition and fate of soluble and digesta particulate associated oxfendazole and its metabolites in the gastrointestinal tract of sheep. International Journal for Parasitology 24, 327–333.CrossRefGoogle ScholarPubMed
Horsfall, J.G. (1956) Principles of fungicidal action. 279 p. Waltham, Mass achusetts, Chronica Britanica Co.Google Scholar
Nari, A. & Eddi, C. (2002) Perspectives and goals. pp. 1–4in Biological control of nematode parasites of small ruminants in Asia. FAO Animal Production and Health Paper, FAO TCP in Mayasia. Rome, FAO.Google Scholar
Samson, R.A. (1974) Paecilomyces and some allied hyphomycetes. Studies in Mycology No. 6. 119 pp. Baarn, Centraalbureau Voor Schimmelcultures.Google Scholar
Sanyal, P.K. (1995) Kinetic disposition of triclabendazole in buffalo compared to cattle. Journal of Veterinary Pharmacology and Therapeutics 18, 370–374.CrossRefGoogle ScholarPubMed
Sanyal, P.K. (1997) Disposition kinetics of albendazole in buffalo and cattle. Journal of Veterinary Pharmacology and Therapeutics 20, 240–242.CrossRefGoogle Scholar
Sanyal, P.K. (2000) Screening for Indian isolates of predacious fungi for use in biological control against nematode parasites of ruminants. Veterinary Research Communications 24, 55–62.CrossRefGoogle ScholarPubMed
Sanyal, P.K. (2001) Control of tropical fasciolosis in cattle and buffaloes in India at the backdrop of its integrated management. Journal of Veterinary Parasitology 15, 13–16.Google Scholar
Sanyal, P.K. (2005) Mycological control of nematode parasites of livestock: from academic interest to reality. Proceedings of the National Academy of Sciences, India 75, 263–271.Google Scholar
Sanyal, P.K., Chauhan, J.B. & Mukhopadhyaya, P.N. (2004) Implications of fungicidal effects of benzimidazole compounds on Duddingtonia flagrans in integrated nematode parasite management in livestock. Veterinary Research Communications 28, 375–385.CrossRefGoogle ScholarPubMed
Sanyal, P.K., De, S., Sarkar, A.K., Patel, N.K., Mandal, S.C. & Pal, S. (2008) Isolation of egg parasitic fungi from Chhattisgarh. Journal of Veterinary Parasitology 22, 1–3.Google Scholar
Stirling, G.R. (1991) Antagonist of nematodes. pp. 50–98in Stirling, G.R. (Ed.) Biological control of plant parasitic nematodes. Wallingford, UK, CAB International.Google Scholar
Theodorides, V.J., Gyurik, R.J., Kingsbury, W.D. & Parish, R.C. (1976) Anthelmintic activity of albendazole against liver flukes, tapeworms, lung and gastrointestinal round worms. Expermentia 32, 702–703.CrossRefGoogle Scholar