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Factors affecting the reliability of the McMaster technique

Published online by Cambridge University Press:  05 June 2009

Alison Dunn  and
Anne Keymer
Alison Dunn
Affiliation:
Department of Zoology,University of Oxford, Oxford OXI 3PS, England.
Anne Keymer
Affiliation:
Department of Zoology,University of Oxford, Oxford OXI 3PS, England.
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Abstract

Factors detraction from the reliability of faecal egg counts based on the McMaster technique include variation in flotation time (interval between loading chamber and counting eggs) and sample dilution (ratio of faecal material to salt solution). We recommend standardization of both these variables as normal laboratory procedure, and propose optima of a 30 minute flotation time and a sample dilution of 15 ml salt solution/ g faeces for use of the McMaster technique in the estimation of the fecundity of Heligmosomoides polygyrus (Nematoda).

Keywords

McMaster techniqueegg countsHeligmosomoides polygyrus
Type
Research Papers
Information
Journal of Helminthology , Volume 60 , Issue 4 , December 1986 , pp. 260 - 262
Copyright
Copyright © Cambridge University Press 1986

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References

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