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Evaluation of antioxidant and oxidant status of goats (Capra aegagrus hircus) naturally infected with Haemonchus contortus

Published online by Cambridge University Press:  14 February 2019

S. Rashid Open the ORCID record for S. Rashid [Opens in a new window]  and
M. Irshadullah Open the ORCID record for M. Irshadullah [Opens in a new window]
S. Rashid*
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202002, India
M. Irshadullah
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202002, India
*
Author for correspondence: S. Rashid E-mail: [email protected]
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Abstract

The present study aimed to assess the antioxidant and oxidant status of goats naturally infected with Haemonchus contortus. Based upon the parasite burden, infection in goats was categorized as heavy (> 500 worms), mild (100–500 worms) or low (< 100 worms). Abomasal tissues from non-infected and infected goats were used for the determination of catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), aspartate (AST) and alanine (ALT) aminotransferases, acid (ACP) and alkaline (ALP) phosphatases, superoxide content (O2−), protein carbonyl (PC), malondialdehyde (MDA) and reduced glutathione (GSH). A significantly higher level of CAT, GST and GR activity and a lower level of GPx activity were recorded in infected compared to non-infected tissue. A significant increase in the level of AST, ALT, ALP and ACP was found in the abomasal tissue of the infected animals, which was related to the worm burden. The oxidative stress markers were also altered, with a significant decline in GSH levels, whereas MDA, PC and O2− concentrations showed a marked increase. In conclusion, it has been demonstrated that haemonchosis in goats resulted in considerable oxidative stress, which was directly related to the worm burden.

Keywords

Antioxidant enzymesgoatsHaemonchus contortusoxidative stress
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e36
Copyright
Copyright © Cambridge University Press 2019 

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References

Abo-Shousha, S, Khalil, SS and Rashwan, EA (1999) Oxygen free radical and nitric oxide production in single or combined human schistosomiasis and fascioliasis. Journal of the Egyptian Society of Parasitology 29, 149–156.Google Scholar
Aebi, H (1974) Catalase. In Bergmeyer, HU (ed.), Methods in Enzymatic Analysis. 2nd edition, Vol. 2. New York, NY: Academic Press, pp. 673–684.Google Scholar
Batra, S et al. (1993) Role of reactive oxygen species in expulsion of Nippostrongylus brasiliensis from rats. Parasitology 106, 185–192.Google Scholar
Ben-Smith, A, Lammas, DA and Behnke, JM (2002) Effect of oxygen radicals and differential expression of catalase and superoxide dismutase in adult Heligmosomoides polygyrus during primary infections in mice with differing response phenotypes. Parasite Immunology 24, 119–129.Google Scholar
Benzer, F and Temizer-Ozan, S (2003) The status of lipid peroxidation, antioxidant enzymes and nitric oxide in sheep infected with Fasciola hepatica. Turkish Journal of Veterinary and Animal Science 27, 657–661.Google Scholar
Bergmeyer, HU, Gawehn, K and Grassl, M (1974) Enzymes as biochemical reagents. In Bergmeyer, HU (ed.), Methods in Enzymatic Analysis. 2nd edition, Vol. 1. New York, NY: Academic Press, pp. 495–497.Google Scholar
Brophy, PM and Pritchard, DI (1994) Parasitic helminth glutathione S-transferases: update on their potential as immuno- and chemotherapeutic targets. Experiment Parasitology 79, 89–96.Google Scholar
Burke, JM et al. (2016) Examination of commercially available copper oxide wire particles in combination with albendazole for control of gastrointestinal nematodes in lambs. Veterinary Parasitology 215, 1–4.Google Scholar
Callahan, HL, Crouch, RK and James, ER (1988) Helminth antioxidant enzymes: a protective mechanism against host oxidants? Parasitology Today 4, 218–225.Google Scholar
Carlberg, I and Mannervik, B (1985) Glutathione reductase. Methods in Enzymology 113, 484–490.Google Scholar
Chiumiento, L and Bruschi, F (2009) Enzymatic antioxidant systems in helminth parasites. Parasitology Research 105, 593–603.Google Scholar
Dalle-Donne, I et al. (2006) Protein carbonylation, cellular dysfunction, and disease progression. Journal of Cellular and Molecular Medicine 10, 389–406.Google Scholar
De Oliveira, RB et al. (2013) Schistosoma mansoni infection causes oxidative stress and alters receptor for advanced glycation end product (RAGE) and tau levels in multiple organs in mice. International Journal of Parasitology 43, 371–379.Google Scholar
Deger, Y et al. (2008) Lipid peroxidation and antioxidant potential of sheep liver infected naturally with distomatosis. Turkiye Parazitoloji Dergisi 32, 23–26.Google Scholar
Derda, M, Wandurska-Nowak, E and Hadas, E (2004) Changes in the level of antioxidants in the blood from mice infected with Trichinella spiralis. Parasitology Research 93, 207–210.Google Scholar
Esmaeilnejad, B et al. (2012) Evaluation of antioxidant status and oxidative stress in sheep naturally infected with Babesia ovis. Veterinary Parasitology 185, 124–130.Google Scholar
Gollapudi, VK and Vardhani, VV (2013) Effect of ancylostomiasis on liver protein, amino acids and GST (glutathione S-transferase) level in male Swiss albino mice. Bioscan 8, 459–462.Google Scholar
Green, MJ and Hill, HA (1984) Chemistry of dioxygen. Methods in Enzymology 105, 3–22.Google Scholar
Habig, WH, Pabst, MJ and Jakoby, WB (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry 249, 7130–7139.Google Scholar
Halliwell, B (1991) Reactive oxygen species in living systems: source, biochemistry and role in human disease. American Journal of Medicine 91, 14–21.Google Scholar
Halliwell, B and Gutteridge, JMC (2007) Free Radicals in Biology and Medicine. 4th edn. New York, NY: Oxford, University Press.Google Scholar
Halprin, K and Ohkawara, A (1967) The measurement of glutathione in human epidermis using glutathione reductase. The Journal of Investigative Dermatology 48, 149–152.Google Scholar
Heidarpour, M et al. (2012) Oxidative stress and trace elements in camel (Camelus dromedaries) with liver cystic echinococcosis. Veterinary Parasitology 187, 459–463.Google Scholar
Heidarpour, M et al. (2013a) Oxidant/antioxidant status in cattle with liver cystic echinococcosis. Veterinary Parasitology 195, 131–135.Google Scholar
Heidarpour, M et al. (2013b) Oxidant/antioxidant balance and trace elements status in sheep with liver cystic echinococcosis. Comparative Clinical Pathology 22, 1043–1049.Google Scholar
Jollow, DJ et al. (1974) Acetaminophen-induced hepatic necrosis. VI. Metabolic disposition of toxic and non-toxic doses of acetaminophen. Pharmacology 12, 251–271.Google Scholar
Khan, S, Saifullah, MK and Abidi, SMA (2013) Pathobiochemical changes in Trichogaster fasciatus fish infected with progenetic metacercariae of Clinostomum complanatum. Journal of Veterinary Parasitology 27, 113–116.Google Scholar
Kolodziejczyk, L, Siemieniuk, E and Skrzydlewska, E (2005) Antioxidant potential of rat liver in experimental infection with Fasciola hepatica. Parasitology Research 96, 367–372.Google Scholar
Kolodziejczyk, L, Siemieniuk, E and Skrzydlewska, E (2006) Fasciola hepatica: effects on antioxidative properties and lipid peroxidation of rat serum. Experimental Parasitology 113, 43–48.Google Scholar
Le Bars, H and Banting, A de L (1976) Pathophysiological studies of experimental Fasciola hepatica infections in sheep and rabbits. In EJL, Soulsby (ed.), Pathophysiology of Parasitic Infection. New York, NY: Academic Press, pp. 75–82.Google Scholar
Levine, RL et al. (1990) Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymology 186, 464–478.Google Scholar
Łuszczak, J, Ziaja-Sołtys, M and Rzymowska, J (2011) Anti-oxidant activity of superoxide dismutase and glutathione peroxidase enzymes in skeletal muscles from slaughter cattle infected with Taenia saginata. Experimental Parasitology 128, 163–165.Google Scholar
Maffei Facino, R et al. (1993) Efficacy of glutathione for treatment of fascioliasis. An investigation in the experimentally infested rat. Arzneimittel-Forschung/Drug Research 43, 455–460.Google Scholar
McMillan, DC et al. (2005) Lipids versus proteins as major targets of pro-oxidant, direct-acting hemolytic agents. Toxicological Science 88, 274–283.Google Scholar
Ohkawa, H, Ohishi, N and Yagi, K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95, 351–358.Google Scholar
Ortolani, EL et al. (2013) Effects of parasitism on cellular immune response in sheep experimentally infected with Haemonchus contortus. Veterinary Parasitology 196, 230–234.Google Scholar
Othman, AA et al. (2016) Atorvastatin and metformin administration modulates experimental Trichinella spiralis infection. Parasitology International 65, 105–112.Google Scholar
Paglia, PE and Valentine, WN (1967) Studies on the quantitation and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine 70, 158–169.Google Scholar
Perry, BD et al. (2002) Investing in Animal Health Research to Alleviate Poverty. 148 pp. International Livestock Research Institute, Nairobi, Kenya.Google Scholar
R Development Core Team (2012) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.r-project.org/.Google Scholar
Rashid, S and Irshadullah, M (2014) Partial characterization of superoxide dismutase activity in the Barber pole worm Haemonchus contortus infecting Capra hircus and abomasal tissue extracts. Asian Pacific Journal of Tropical Biomedicine 4, 718–724.Google Scholar
Rashid, S and Irshadullah, M (2018) Epidemiology and seasonal dynamics of adult Haemonchus contortus in goats of Aligarh, Uttar Pradesh, India. Small Ruminant Research 161, 63–67.Google Scholar
Reitman, S and Frankel, S (1957) Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology 28, 56–63.Google Scholar
Rushmore, TH, Morton, MR and Pickett, CB (1991) The antioxidant responsive element. Activation by oxidative stress and identification of the DNA consensus sequence required for functional activity. Journal of Biological Chemistry 266, 11632–11639.Google Scholar
Saleha, MA, Mahranb, OM and Al-Salahy, BM (2011) Corpuscular oxidation in newborn crossbred calves naturally infected with Theileria annulata. Veterinary Parasitology 182, 193–200.Google Scholar
Sanchez-Campos, S et al. (1999) Oxidative stress and changes in liver antioxidant enzymes induced by experimental dicroceliosis in hamsters. Parasitology Research 85, 468–474.Google Scholar
Shan, XQ, Aw, TY and Jones, DP (1990) Glutathione-dependent protection against oxidative injury. Pharmacology and Therapeutics. 47, 61–71.Google Scholar
Siemieniuk, E, Kolodziejczyk, L and Skrzydlewska, E (2008) Oxidative modifications of rat liver cell components during Fasciola hepatica infection. Toxicology Mechanisms and Methods 18, 519–524.Google Scholar
Smith, NC and Bryant, C (1989) Free radical generation during primary infections with Nippostrongylus brasiliensis. Parasite Immunology 11, 147–160.Google Scholar
Southern, PA and Powis, G (1988) Free radicals in medicine. I. Chemical nature and biologic reactions. Mayo Clinic Proceedings 63, 381–389.Google Scholar
Spector, T (1978) Refinement of the Coomassie blue method of protein quantitation. Analytical Biochemistry 86, 142–146.Google Scholar
Urquhart, GM et al. (2000) Veterinary Parasitology. 2nd edn. 307 pp. London: Blackwell Science Ltd.Google Scholar
Weydert, CJ and Cullen, JJ (2010) Measurement of superoxide dismutase, catalase, and glutathione peroxidase in cultured cells and tissue. Nature 5, 51–66.Google Scholar