Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-02T23:03:43.505Z Has data issue: false hasContentIssue false
  • English
  • Français

Eosinophilia, IL-5 level and recovery of larvae in IL-5 transgenic mice infected with Toxocara canis

Published online by Cambridge University Press:  05 June 2009

K. Sugane ,
Y. Kusama ,
M. Takamoto ,
A. Tominaga  and
K. Takatsu
K. Sugane
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto City 390, Japan
Y. Kusama
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto City 390, Japan
M. Takamoto
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto City 390, Japan
A. Tominaga
Affiliation:
Department of Medical Biology, Kochi Medical School, Kochi, Japan
K. Takatsu
Affiliation:
Department of Immunology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

Eosinophil counts, interleukin 5 (IL-5) concentrations in peripheral blood and larval recovery after infection with Toxocara canis were compared between IL-5 transgenic (Tg) and nontransgenic counterparts, C3H/HeN mice. In Tg mice characterized by a constant high level of peripheral eosinophils, eosinophils in peripheral blood fell to the lowest level on day 4 of a T. canis infection and then returned to the preinfection level on day 14. Serum IL-5 level fell to the lowest level on day 4 of infection, then recovered rapidly and peaked on day 14 postinfection. In contrast, the eosinophil and IL-5 levels in the peripheral blood peaked on days 11 and 7 of infection, respectively in C3H/HeN mice. The degree of eosinophil infiltration into the lung 4 days after infection was far more pronounced in Tg than C3H/HeN mice. The highest number of larvae recovered from the lungs of infected Tg and C3H/HeN mice occurred on day 4 postinfection. Strains of Tg and C3H/HeN mice vaccinated with excretory and secretory (ES) antigens of T. canis larvae were infected with T. canis and the recovery of larvae on day 21 analysed. There were no significant differences in the mean number of larvae recovered from nonvaccinated Tg and C3H/HeN mice or vaccinated Tg and C3H/HeN mice. However, significant differences were demonstrated in the mean total number of larvae recovered from vaccinated and nonvaccinated Tg or C3H/HeN mice. These results suggest that immunocompetent cells other than eosinophils may play a significant role in the expulsion and killing of T. canis larvae in infected mice.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 2 , June 1996 , pp. 153 - 158
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Butterworth, A.E., David, J.R., Franks, D., Mahmoud, A.A.F., David, P.H., Sturrock, R.F. & Houba, V. (1977) Antibody-dependent eosinophil-mediated damage to 51Cr-labelled schistosomula of Schistosoma mansoni: damage by purified eosinophils. Journal of Experimental Medicine 145, 136–150.CrossRefGoogle Scholar
Capron, M., Bazin, H., Joseph, M. & Capron, A. (1981) Evidence for IgE-dependent cytotoxicity by rat eosinophils. Journal of Immunology 126, 1764–1768.CrossRefGoogle ScholarPubMed
Coffman, R.L., Seymour, B.W.P., Hudak, S., Jackson, J. & Rennick, D. (1989) Antibody to interleukin-5 inhibits helminth-induced eosinophilia in mice. Science 245,308–311.CrossRefGoogle ScholarPubMed
DeSavigny, D.H. (1975) In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. Journal of Parasitology, 61, 781–782.CrossRefGoogle Scholar
Discombe, G. (1946) Criteria of eosinophilia. Lancet I, 195–196.CrossRefGoogle ScholarPubMed
Fairbairn, D. (1961) The in vitro hatching of Ascaris lumbricoides. Canadian Journal of Zoology 39, 153–167.CrossRefGoogle Scholar
Grove, D.I., Mahmoud, A.A.F. & Warren, K.S. (1977) Eosinophils and resistance to Trichinella spiralis. Journal of Experimental Medicine 145, 755–759.CrossRefGoogle ScholarPubMed
Herndon, F.J. & Kays, S.G. (1992) Depletion of eosinophils by anti-IL-5 monoclonal antibody treatment of mice infected with Trichinella spiralis does not alter parasite burden or immunologic resistance to reinfection. Journal of Immunology 149, 3642–3647.CrossRefGoogle ScholarPubMed
Hillyer, G.V. & De Rios, I.G.(1979) The enzyme-linked immunosorbent assay (ELISA) for the immune-diagnosis of schistosomiasis. American Journal of Tropical Medicine and Hygiene, 28, 237–241.CrossRefGoogle Scholar
Kazura, J.W. & Aikawa, M. (1980) Host defence mechanisms against Trichinella spiralis infection in the mouse: eosinophil-mediated destruction of newborn larvae in vitro. Journal of Immunology 124, 355–361.CrossRefGoogle ScholarPubMed
Kephart, G.M., Gleich, G.J., Connor, D.H., Gibson, D.W. & Ackermann, S.J. (1984) Deposition of eosinophil granule major basic protein onto microfilariae of Onchocerca volvulus in the skin of patients treated with diethylcarbamazine. Laboratory Investigation 50, 51–61.Google ScholarPubMed
Limaye, A.P., Abrams, J.S., Silver, J.E., Ottesen, E.A. & Nutman, T.B. (1990) Regulation of parasite-induced eosinophilia: selectively increased interleukin 5 production in helminth-infected patient. Journal of Experimental Medicine 172, 399–405.CrossRefGoogle Scholar
Mahmoud, A.A.F., Warren, K.S. & Peters, P.A. (1975) A role for the eosinophil in acquired resistance to Schistosoma mansoni infection as determined by antieosinophil serum. Journal of Experimental Medicine 142, 805–813.CrossRefGoogle ScholarPubMed
Nicholas, B.W.L., Stewart, A.C. & Mitchell, G.F. (1984) Antibody responses to Toxocara canis using sera from parasite-infected mice and protection from toxocariasis by immunization with ES antigens. Australian Journal of Experimental Biology and Medical Science 62, 619–626.CrossRefGoogle ScholarPubMed
Olson, L.J. (1962) Organ distribution of Toxocara canis larvae in normal mice and in mice previously infected with Toxocara, Ascaris or Trichinella. Texas Reports on Biology and Medicine 20, 651–657.Google ScholarPubMed
Oshima, T. (1961) Standardization of techniques for infecting mice with Toxocara canis and observations on the normal migration routes of the larvae. Journal of Parasitology 47, 662–656.CrossRefGoogle ScholarPubMed
Oshima, T. (1976) Observations of the age resistance, eosinophilia, and larval behavior in the helminth-free beagles infected with Toxocara canis. Japanese Journal of Parasitology 25, 447–455.Google Scholar
Ramalho-Pinto, F.J., McLaren, D.J. & Smithers, S.R. (1978) Complement-mediated killing of schistosomula of Schistosoma mansoni by rat eosinophils in vitro. Journal of Experimental Medicine 147, 147–156.CrossRefGoogle ScholarPubMed
Sher, A., Coffman, R.L., Hyeny, S. & Cheener, A.W. (1990) Ablation of eosinophil and IgE responses with anti-IL-5 or anti-IL-4 antibodies fails to affect immunity against Schistosoma mansoni in the mouse. Journal of Immunology 145, 3911–3916.CrossRefGoogle ScholarPubMed
Strath, M., Dent, L. & Sanderson, C. (1992) Infection of IL-5 transgenic mice with Mesocestoides corti induces very high levels of IL-5 but depressed production of eosinophils. Experimental Hematology 20, 229–234.Google ScholarPubMed
Sugane, K. & Oshima, T. (1982) Eosinophilia, granuloma formation and migratory behaviour of larvae in the congenitally athymic mouse infected with Toxocara canis. Parasite Immunology 4, 307–318.CrossRefGoogle ScholarPubMed
Tominaga, A., Takaki, S., Koyama, N., Katoh, S., Matsumoto, R., Migita, M., Hitoshi, Y., Hosoya, Y., Yamauchi, S., Kanai, Y., Miyazaki, J., Usuku, G., Yamamura, K. & Takatsu, K. (1991) Transgenic mice expressing a B cell growth and differentiation factor gene develop eosinophilia and autoantibody production. Journal of Experimental Medicine 173, 429–437.CrossRefGoogle Scholar
Yamaguchi, Y., Suda, T., Suda, J., Eguchi, M., Miura, Y., Harada, N., Tominaga, A. & Takatsu, K. (1988a) Purified interleukin 5 supports the terminal differentiation and proliferation of murine eosinophilic precursors. Journal of Experimental Medicine 167, 43–56.CrossRefGoogle ScholarPubMed
Yamaguchi, Y., Hayashi, Y., Sugama, Y., Miura, Y., Kasahara, T., Kitamura, S., Torisu, M., Mita, S., Tominaga, A., Takatau, K. & Suda, T. (1988b) Highly purified murine interleukin 5(IL-5) stimulates eosinophil function and prolongs in vitro survival: IL-5 as an eosinophil chemotactic factor. Journal of Experimental Medicine 167, 1737–1742.CrossRefGoogle ScholarPubMed
Yamaguchi, Y., Matsui, T., Kasahara, T., Etoh, S., Tominaga, A., Takatsu, K., Miura, Y. & Suda, T. (1990) In vitro changes of hemopoietic progenitors and the expression of Interleukin 5 gene in eosinophilic mice infected with Toxocara canis. Experimental Hematology 18, 1152–1157.Google ScholarPubMed