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Enzyme-linked immunosorbent assay, immunoblot analysis and RAST fluoroimmunoassay analysis of serum responses against crude larval antigens of Anisakis simplex in a Spanish random population

Published online by Cambridge University Press:  05 June 2009

L. García-Palacios ,
M.L. González ,
M.I. Esteban ,
E. Mirabent ,
M.J. Perteguer  and
C. Cuéllar
L. García-Palacios
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
M.L. González
Affiliation:
Unidad de Alergia, Hospital General de Segovia, Spain
M.I. Esteban
Affiliation:
Unidad de Alergia, Hospital General de Segovia, Spain
E. Mirabent
Affiliation:
Laboratorio de Análisis Clínicos, Hospital General de Segovia, Spain
M.J. Perteguer
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
C. Cuéllar
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
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Abstract

The results obtained in a study of seroprevalence by means of ELISA and immunoblot with crude larval extracts of Anisakis simplex using 1008 human sera from Spanish people showing no clinical suspicion of anisakidosis are given. For the evaluation of the results obtained by ELISA the Diagnostic Index (DI) was used, as the ratio between the optical density resulting from the test serum and the optical density of the negative control. Forty-seven sera showed DIs between 1.5 and 2, and 14 sera were greater than 2. After comparison of the immunoblot analysis with the immunorecognition pattern of a human anisakidosis reference serum, a diagnostic criterion could be established for those sera that, at a 1/100 dilution, showed a DI by ELISA greater than 1.5. Seven of 14 selected sera with DIs in ELISA higher than 1.3 showed anti-Anisakis specific IgE antibodies by RAST fluoroimmunoassay.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 4 , December 1996 , pp. 281 - 289
Copyright
Copyright © Cambridge University Press 1996

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