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Em18 and Em16, new serologic marker epitopes for alveolar echinococcosis in Western blot analysis, are the only two epitopes recognized by commercially available weak positive (cut off) sera for Em2plus-ELISA

Published online by Cambridge University Press:  05 June 2009

A. Ito ,
Y. Osawa ,
M. Nakao ,
T. Horii ,
M. Okamoto ,
M. Itoh  and
T. Yamashita
A. Ito
Affiliation:
Department of Parasitology, Gifu University School of Medicine, Gifu 500, Japan
Y. Osawa
Affiliation:
Department of Parasitology, Gifu University School of Medicine, Gifu 500, Japan
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078, Japan
T. Horii
Affiliation:
Department of Molecular Parasitology, Institute of Microbial Diseases, Osaka University, Suita 565, Japan
M. Okamoto
Affiliation:
Department of Experimental Animals, Osaka University School of Medicine, Suita 565, Japan
M. Itoh
Affiliation:
Department of Medical Zoology, Medical School, Nagoya City University, Nagoya 467, Japan
T. Yamashita
Affiliation:
Department of Virology, Aichi Prefectural Institute of Public Health, Nagoya 462, Japan
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Abstract

The assay system for antibody responses against Em2, the most specific antigen for serodiagnosis of alveolar echinococcosis (AE), has been established by enzyme-linked immunosorbent assay (ELISA) but not by Western blot assay, since Em2 antigen is not protein but carbohydrate in nature. Recently we reported that previously undescribed protein epitopes, designated Em18 and Em16 due to their molecular weights, were good serologic markers for AE by Western blot analysis. It has been shown that Em18 and Em16 are the only two epitopes recognized by commercially available weak positive (cut off) sera for the Em2plus-ELISA.

Type
Research Note
Information
Journal of Helminthology , Volume 69 , Issue 4 , December 1995 , pp. 369 - 371
Copyright
Copyright © Cambridge University Press 1995

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References

Craig, P.S., Deshan, L., Macpherson, C.N.L., Dazhong, S., Reynolds, D., Barnish, G., Gottstein, B. & Zhirong, W. (1992) A large focus of alveolar echinococcosis in central China. Lancet 340, 826–831.CrossRefGoogle ScholarPubMed
Felleisen, R. & Gottstein, B.(1993) Echinococcus multilocularis: molecular and immunochemical characterization of diagnostic antigen II/3–10. Parasitology 107, 335–342.CrossRefGoogle ScholarPubMed
Gottstein, B. (1985) Purification and characterization of a specific antigen from Echinococcus multilocularis. Parasite Immunology 7, 201–212.Google Scholar
Gottstein, B.(1992) Echinococcus multilocularis infections: immunology and immunodiagnosis. Advances in Parasitology 31, 321–380.CrossRefGoogle ScholarPubMed
Gottstein, B., Jacquier, P., Bresson-Hadni, S. & Eckert, J. (1993) Improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the EM2plus antigen. Journal of Clinical Microbiology 31, 373–376.Google Scholar
Gottstein, B., Wunderlin, E. & Tanner, I. (1994) Echinococcus multilocularis: parasite-specific humoral and cellular immune response subsets in mouse strains susceptible (AKR, C57B1/6J) or –resistant— (C57B1/10) to secondary alveolar echinococcosis. Clinical and Experimental Immunology 96, 245–252.Google Scholar
Ito, A., Nakao, M., Kutsumi, H., Lightowlers, M.W., Itoh, M. & Sato, S. (1993a) Serodiagnosis of alveolar hydatid disease by Western blotting. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 170–172.Google Scholar
Ito, A., Wang, X. & Liu, Y. (1993b) Differential serodiagnosis of alveolar and cystic hydatid disease in the People's Republic of China. American Journal of Tropical Medicine and Hygiene 49, 208–213.Google Scholar
Ito, A., Schantz, P.M. & Wilson, J.F. (1995) Em18, a new serodiagnostic marker for differentiation of active and inactive cases of alveolar hydatid disease. American Journal of Tropical Medicine and Hygiene 52, 41–44.CrossRefGoogle ScholarPubMed
Schantz, P.M., Gottstein, B., Ammann, R. & Lanier, A. (1991) Hydatid and the Arctic. Parasitology Today 7, 35–36.CrossRefGoogle Scholar
Wen, H., New, R.R.C. & Craig, P.S. (1992) Diagnosis and treatment of human hydatidosis. British Journal of Clinical Pharmacology 35, 565–574.Google Scholar
Wen, H. & Craig, P.S. (1994) IgG subclass responses in human cystic and alveolar echinococcosis. American Journal of Tropical Medicine and Hygiene 51, 741–748.CrossRefGoogle ScholarPubMed