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Electron Microscope and Histochemical Observations on the Daughter Sporocyst of Schistosoma mattheei and Schistosoma bovis

Published online by Cambridge University Press:  05 June 2009

G. K. Kinoti ,
R. G. Bird  and
Mary Barker
G. K. Kinoti
Affiliation:
London School of Hygiene and Tropical Medicine
R. G. Bird
Affiliation:
London School of Hygiene and Tropical Medicine
Mary Barker
Affiliation:
London School of Hygiene and Tropical Medicine
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Extract

Electron-microscopical, histological and histochemical observations were carried out on the body wall of the daughter sporocyst stage of Schistosoma mattheei and S. bovis. Electron microscopy revealed that the body wall consists of a continuous outer layer of cytoplasm, collagen fibres, fibroblasts and a layer of somatic cells which are apparently continuous with the cytoplasmic layer. This layer forms numerous microvilli at the surface.

Pronounced alkaline phosphatase activity was found in the sporocyst body wall, but no evidence of esterases was found in the parasite although esterase activity was readily demonstrated in the snail tissue that the schistosome parasitized.

It is concluded that the passage of substances such as glucose across the surface of the sporocyst is an active process probably mediated by enzymes and that little, if any, lipid metabolism occurs in the sporocyst stage.

Type
Research Article
Information
Journal of Helminthology , Volume 45 , Issue 2-3 , June 1971 , pp. 237 - 244
Copyright
Copyright © Cambridge University Press 1971

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References

Barka, T. and Anderson, P. J., 1963.—Histochemistry: theory, practice and bibliography. New York. Harper and Row.Google Scholar
Brand von, T., 1966.—Biochemistry of parasites. London and New York. Academic Press.Google Scholar
Burstone, M. S., 1964.—Enzyme histochemistry and cytochemistry. In: Bourne, G. H. (Ed.), “Cytology and Cell Physiology.” London and New York. Academic Press.Google Scholar
Cort, W. W., Ameel, D. J. and Van Der Woude, A., 1954.—“Germinal development in sporocysts and rediae of digenetic trematodes.” Expi Parasit., 3, 185–225.CrossRefGoogle ScholarPubMed
Erasmus, D. A. and Ohman, C., 1963.—“The structure and function of the adhesive organ in strigeid trematodes.” Ann. N.Y. Acad. Sci., 113, 7–35.Google Scholar
Gorr, E., 1956.—A practical manual of medical and biological staining techniques. London. Leonard Hill (Books) Ltd.Google Scholar
Kinoti, G. K., 1967,—“Studies on some factors affecting the development of schistosomes in their molluscan hosts.” Ph.D. thesis London University.Google Scholar
Kinoti, G. K., 1971.—“Observations on the infection of bulinid snails with Schisto soma matheei. II. The mechanism of resistance to infection.” Parasitology, 62, 161–170.CrossRefGoogle Scholar
Lee, D. L., 1966.—“The structure and composition of the helminth cuticle.” In A dvances in parasitology, Vol. I V. London and New York. Academic Press.Google Scholar
Muller, H., 1965.—“Histochemical localization of phosphatases and esterases in Australorbis glabralus.” Proc. zool. Soc. Lond., 144, 229–237.CrossRefGoogle Scholar
Noviroff, A. B., 1955.—Histochemical and cytochemical staining methods. In Mellors, R. C., “Analytical cytology“, New York, Toronto, London. McGraw-Hill Book Company.Google Scholar
Pearce, A. G. E., 1960.—Hislochemistry: theoretical and applied. (2nd edition.) London. J. & A. Churchill.Google Scholar
Wiseman, G., 1964.—Absorption from the intestine. London and New York. Academic Press.Google Scholar