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The effects of temperature and salinity on the longevity of Opisthorchis viverrini cercariae: a climate change concern

Published online by Cambridge University Press:  23 June 2020

S. Prasopdee Open the ORCID record for S. Prasopdee [Opens in a new window] ,
J. Kulsantiwong ,
T. Sathavornmanee Open the ORCID record for T. Sathavornmanee [Opens in a new window]  and
V. Thitapakorn
S. Prasopdee*
Affiliation:
Research Group in Development of Diagnosis and Targeted Therapeutics for Opisthorchis viverrini and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University, Klong Luang, Pathum Thani12120, Thailand
J. Kulsantiwong
Affiliation:
Department of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani41000, Thailand
T. Sathavornmanee
Affiliation:
Research Group in Development of Diagnosis and Targeted Therapeutics for Opisthorchis viverrini and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University, Klong Luang, Pathum Thani12120, Thailand
V. Thitapakorn
Affiliation:
Research Group in Development of Diagnosis and Targeted Therapeutics for Opisthorchis viverrini and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University, Klong Luang, Pathum Thani12120, Thailand
*
Author for correspondence: S. Prasopdee, E-mail: [email protected]
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Abstract

Research on the effects of environmental factors influenced by climate change on parasite transmissibility is an area garnering recent attention worldwide. However, there is still a lack of studies on the life cycle of Opisthorchis viverrini, a carcinogenic trematode found in countries of the Lower Mekong subregion of Lao PDR, Cambodia, Myanmar, Vietnam and Thailand. To evaluate the influences of environmental factors water temperature and salinity on the transmissibility of the liver fluke O. viverrini through cercarial stage, longevity of O. viverrini cercaria was examined at different experimental temperatures (22°C, 30°C and 38°C) and salinities (2.5 parts per thousand (PPT), 3.75 PPT and 5 PPT). The results reveal that different temperatures have statistically significant effects on cercarial longevity. The cercariae exhibited a thermostability zone ranging between 22°C and 30°C. Cercarial longevity was significantly shortened when water temperatures reached 38°C. Salinity also plays a key role in cercarial longevity, with cercarial survival significantly shorter at a salinity of 3.75 PPT than at 2.5 PPT and 5 PPT. A combined analysis of salinity and temperature revealed unique trends in cercarial longevity. At all experimental salinities, cercarial longevity was lowest when incubated in 38°C, but statistically significant from cercarial longevity at temperatures of 22°C and 30°C, and salinities of 2.5 PPT and 5 PPT. The results suggest that higher temperatures negatively impact parasite longevity. This reflects that O. viverrini transmission patterns may be impacted by changes in water temperature and salinity resulting from climate change.

Keywords

Opisthorchis viverrinitemperaturesalinityclimate changecercarial transmissioncholangiocarcinoma
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e165
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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