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Echinococcus multilocularis: Identification of proteins inducing antibody formation in metacestode infection

Published online by Cambridge University Press:  18 November 2009

K. Lingelbach
Affiliation:
Institutfur Tropenhygien, Im Neuenheimer Feld 324, 69 Heidelberg, West Germany

Abstract

An approach to the identification of parasite proteins which are immunogenic in natural infections is described, using the infection with the larval stage of Echinococcus multilocularis as a parasite model. Metacestode proteins were separated by SDS-polyacrylamide gel electrophoresis, and transferred electrophoretically to nitrocellulose sheets (Western blotting). Subsequently, immune recognition of the proteins was performed with various host sera and antigen-antibody complexes were detected enzymatically. Using homologous antisera, different patterns of immunogenic bands were revealed by sera of different host species. Cross-reactions with sera from individuals infected with unrelated helminths were analysed. Four proteins of E. multilocularis which failed to show any cross-reaction were identified.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1984

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References

REFERENCES

Burnette, W.N. (1981) “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry, 112, 195203.Google Scholar
Chordi, A. & Kagan, I.G. (1965) Identification and characterization of antigenic components of sheep hydatid fluid by immunoelectrophoresis. Journal of Parasitology, 51, 6371.Google Scholar
Craig, P.S., Hocking, R.E., Mitchell, G.F. & Rickard, M.D. (1981) Murine hybridomaderived antibodies in the processing of antigens for the immunodiagnosis of hydatid (Echinococcus granulosus) infection in sheep. Parasitology, 83, 303317.Google Scholar
Hinz, E. (1972) Die Entwicklungdes sekundären Echinococcus multilocularis in der experimentell infizierten Maus. Einc quantitative Analyse. Zeitschrift Jür Tropenmedizin und Parasitologie, 23, 256265.Google Scholar
Hinz, E., Diesfeld, H.J., Gehrig, H. & Kirsten, C. (1981) Serologische Kreuzreaktionen zwischen experimcntell mit Echinococcus multilocularis oder Dipetalonema viteae infizierten Nagetieren. Tropenmedizin und Parasitologie, 32, 247249.Google Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London, 111, 680685.Google Scholar
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265275.Google Scholar
Matossian, R.M. (1977) The immunological diagnosis of human hydatid disease. Transactions of the Royal Society of Tropical Medicine and Hygiene, 71, 101104.Google Scholar
Mitchell, G.F., Cruise, K.M., Chapman, C.B., Anders, R.F. & Howard, M.C. (1979) Hybridoma antibody immunoassays for the detection of parasite infection: development of a model system using a larval cestode infection in mice. Australian Journal of Experimental Biology and Medical Science, 57, 287302.Google Scholar
Rickard, M.D. (1979) The immunological diagnosis of hydatid disease. Australian Veterinary Journal, 55, 99104.Google Scholar
Ruppel, A. & Cioli, D. (1977) A comparative analysis of various developmental stages of Schistosoma mansoni with respect to their protein composition. Parasitology, 75, 339343.CrossRefGoogle ScholarPubMed
Shapiro, A.L., Vinuela, E. & Maizel, J.V. (1967) Molecular weight estimation of polypeptide chains by clectrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications, 28, 815820.Google Scholar
Speiser, F. (1980) Application of the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of filariasis and echinococcosis. Tropenmedizin und Parasitologie, 31, 459466.Google Scholar
Studier, F.W. (1973) Analysis of bacteriophage T7 early RNAs and proteins on slabs gels.Journal of Molecular Biology, 79, 237248.Google Scholar
Towbin, H., Staehelin, T. & Gordon, J. (1979) Electrophorctic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America, 76, 43504354.Google Scholar
Varela-Diaz, V.M., Coltorti, E A., Ricardes, M.I., Guisantes, J.A. & Yarzabal, L.A. (1974) The immunoelectrophoretic characterization of the sheep hydatid cyst fluid antigens. American Journal of Tropical Medicine and Hygiene, 23, 10921096.Google Scholar
Weber, K. & Osborn, M. (1969) The reliability of molecular weight determinations by dodecyl sulfatepolyacrylamide gel electrophoresis. Journal of Biological Chemistry, 244, 44064412.Google Scholar
Yong, W.K. & Heath, D.D. (1979) “Arc 5” antibodies in sera of sheep infected with Echinococcus granulosus, Taenia hydatigena and Taenia ovis. Parasite Immunology, 1, 2738.CrossRefGoogle ScholarPubMed