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Echinococcus multilocularis: Identification of proteins inducing antibody formation in metacestode infection

Published online by Cambridge University Press:  18 November 2009

K. Lingelbach
Affiliation:
Institutfur Tropenhygien, Im Neuenheimer Feld 324, 69 Heidelberg, West Germany

Abstract

An approach to the identification of parasite proteins which are immunogenic in natural infections is described, using the infection with the larval stage of Echinococcus multilocularis as a parasite model. Metacestode proteins were separated by SDS-polyacrylamide gel electrophoresis, and transferred electrophoretically to nitrocellulose sheets (Western blotting). Subsequently, immune recognition of the proteins was performed with various host sera and antigen-antibody complexes were detected enzymatically. Using homologous antisera, different patterns of immunogenic bands were revealed by sera of different host species. Cross-reactions with sera from individuals infected with unrelated helminths were analysed. Four proteins of E. multilocularis which failed to show any cross-reaction were identified.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1984

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