Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-02T22:26:44.906Z Has data issue: false hasContentIssue false

Duddingtonia flagrans, Monacrosporium thaumasium and Pochonia chlamydosporia as possible biological control agents of Oxyuris equi and Austroxyuris finlaysoni

Published online by Cambridge University Press:  02 July 2009

F.R. Braga
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
J.V. Araújo*
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
A.R. Silva
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
J.M. Araujo
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
R.O. Carvalho
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
A.K. Campos
Affiliation:
Univiçosa, Viçosa, Brazil
A.O. Tavela
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
S.R. Ferreira
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
L.N. Frassy
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
C.D.F. Alves
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG36570-000, Brazil
*

Abstract

The action of four fungal isolates of the species Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34a) and Pochonia chlamydosporia (VC1 and VC4) on eggs of Oxyuris equi and Austroxyuris finlaysoni was evaluated in two assays (A and B). Eggs of O. equi (Test A) and A. finlaysoni (Test B) were plated on Petri dishes with 2% water-agar with grown fungal isolates and control without fungus. After 5, 10 and 15 days, 100 eggs were collected and classified according to the following parameters: type 1 effect, physiological and biochemical effect without morphological damage to the eggshell; type 2 effect, lytic effect with morphological alteration of the eggshell and embryo; and type 3 effect, lytic effect with morphological alteration of the eggshell and embryo, hyphal penetration and internal egg colonization. Pochonia chlamydosporia isolates VC1 and VC4 showed ovicidal activity for type 1, 2 and 3 effects on eggs of O. equi and eggs of A. finlaysoni. In vitro assays A and B showed that P. chlamydosporia had a negative influence on eggs of O. equi and A. finlaysoni and can be considered as a potential biological control agent of nematodes.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Araújo, J.V., Santos, M.A. & Ferraz, S. (1995) Efeito ovicida de fungos nematófagos sobre ovos embrionados de Toxocara canis. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 47, 3242.Google Scholar
Araújo, J.V., Mota, M.A. & Campos, A.K. (2004) Controle biológico de helmintos parasitos de animais por fungos nematófagos. Revista Brasileira de Parasitologia Veterinária 13, 165170.Google Scholar
Araújo, J.V., Braga, F.R., Araújo, J.M., Silva, A.R. & Tavela, A.O. (2008) In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Ascaris suum eggs. Parasitology Research 102, 787790.CrossRefGoogle ScholarPubMed
Araújo, J.M., Araújo, J.V., Braga, F.R., Carvalho, R.O., Silva, A.R. & Campos, A.K. (2009) Interaction and ovicidal activity of nematophagous fungus Pochonia chlamydosporia on Taenia saginata eggs. Experimental Parasitology 121, 338341.CrossRefGoogle ScholarPubMed
Ayres, M., Ayres, J.R.M., Ayres, D.L. & Santos, A.S. (2003) Aplicações estatísticas nas áreas de ciências biológicas. 290 pp. Brasília, CNPq.Google Scholar
Bonuti, M.R., Nascimento, A.A., Mapeli, E.B. & Arantes, I.G. (2002) Gastrointestinal helminths of capybara (Hydrochoerus hydrochaeris) from the Paiaguás subregion, in the floodplain of Mato Grosso do sul, Brasil. Ciências Agrárias 23, 5762.CrossRefGoogle Scholar
Bowman, D.D., Lynn, R.C., Eberhard, M. & Alacaraz, L. (2006) Parasitologia Veterinária de Georgis. pp. 115130. 8th edn.Rio de Janeiro, Editora Manole.Google Scholar
Braga, F.R., Araújo, J.V., Campos, A.K., Carvalho, R.O., Silva, A.R., Tavela, A.O. & Maciel, A.S. (2007) Observação in vitro da ação dos isolados fúngicos Duddingtonia flagrans, Monacrosporium thaumasium e Verticillium chlamydosporium sobre ovos de Ascaris lumbricoides (Lineu, 1758). Revista da Sociedade Brasileira de Medicina Tropical 40, 356358.CrossRefGoogle ScholarPubMed
Braga, F.R., Araújo, J.V., Campos, A.K., Carvalho, R.O., Silva, A.R. & Tavela, A.O. (2008) In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Fasciola hepatica eggs. World Journal of Microbiology and Biotechnology 24, 15591564.CrossRefGoogle Scholar
Burger, H.J. & Stoye, M. (1978) Parasitological problems associated with recycling of animal excretions. In Kelly, W.R. (Ed.) Animal and human hazards associated with the utilization of animal effluents. Luxembourg, Office for the Official Publications of the European Communities.Google Scholar
Gams, W. & Zare, R. (2001) A revision of Verticillium sect. Prostrata. III. Generic classification. Nova Hedwigia 73, 329337.CrossRefGoogle Scholar
Gomes, D.C., Cruz, R.P. & Vicente, J.J. (2003) Nematode parasites of marsupials and small rodents from the Brazilian Atlantic Forest in the State of Rio de Janeiro, Brazil. Revista Brasileira de Zoologia 20, 699707.CrossRefGoogle Scholar
Hugot, J.P. & Bougnoux, M.E. (1988) Estude morphologique de Austroxyuris finlaysoni (Oxyuridae, Nematoda), parasite de Petauroides volans (Petauridae, Marsupiala). Systematic Parasitology 11, 113122.CrossRefGoogle Scholar
Larsen, M. (1999) Biological control of helminths. International Journal for Parasitology 29, 139146.CrossRefGoogle ScholarPubMed
Lysek, H. & Sterba, J. (1991) Colonization of Ascaris lumbricoides eggs by the fungus Verticillium chlamydosporium Goddard. Folia Parasitologica 38, 255259.Google ScholarPubMed
Lysek, H., Fassatiová, O., Pineda, N.C. & Hernández, N.L. (1982) Ovicidal fungi in soils from Cuba. Folia Parasitologica 29, 265270.Google ScholarPubMed
Morgan-Jones, G., White, J.F. & Rodriguez-Kábana, R. (1983) Phytonematode pathology: ultrastructural studies. I: Parasitism of Meloidogyne arenaria eggs by Verticillium chlamydosporium. Nematropica 13, 245260.Google Scholar
Nordbring-Hertz, B., Jansson, H.B. & Tunlid, A. (2002) Nematophagous fungi. Encyclopedia of life sciences. 10 pp. Basingstoke, Macmillan Publishers.Google Scholar
Sanyal, P.K., Sarkar, A.K., Patel, N.K., Mandal, S.C. & Pal, S. (2008) Formulation of a strategy for the application of Duddingtonia flagrans to control caprine parasitic gastroenteritis. Journal of Helminthology 82, 169174.CrossRefGoogle ScholarPubMed
Silva, A.V.M. & Massara, C.L. (2005) Ascaris lumbricoides. pp. 253259 in Neves, D.P. (Ed.) Parasitologia humana. Rio de Janeiro, Atheneu.Google Scholar
Stirling, G.R. & West, L.M. (1991) Fungal parasites of root-knot nematodes eggs from tropical and sub tropical regions of Australia. Australasian Plant Pathology Society 20, 149154.CrossRefGoogle Scholar
Urquhart, G.M., Armour, J., Duncan, J.L., Dunn, A.M. & Jennings, F.W. (1998) Parasitologia Veterinária. 273 pp. 2nd edn.Rio de Janeiro, Guanabara & Koogan.Google Scholar