Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-02T18:51:57.522Z Has data issue: false hasContentIssue false
  • English
  • Français

Duddingtonia flagrans chlamydospores in nutritional pellets: effect of storage time and conditions on the trapping ability against Haemonchus contortus larvae

Published online by Cambridge University Press:  16 August 2013

J.A. Fitz-Aranda ,
P. Mendoza-de-Gives ,
J.F.J. Torres-Acosta ,
E. Liébano-Hernández ,
M.E. López-Arellano ,
C.A. Sandoval-Castro  and
H. Quiroz-Romero
J.A. Fitz-Aranda
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla, No. 8534, Col Progreso, Jiutepec, Morelos, México
P. Mendoza-de-Gives*
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla, No. 8534, Col Progreso, Jiutepec, Morelos, México
J.F.J. Torres-Acosta*
Affiliation:
FMVZ, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Km. 15.5 carretera Mérida-Xmatkuil, Mérida, Yucatán, México
E. Liébano-Hernández
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla, No. 8534, Col Progreso, Jiutepec, Morelos, México
M.E. López-Arellano
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla, No. 8534, Col Progreso, Jiutepec, Morelos, México
C.A. Sandoval-Castro
Affiliation:
FMVZ, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Km. 15.5 carretera Mérida-Xmatkuil, Mérida, Yucatán, México
H. Quiroz-Romero
Affiliation:
FMVZ, Universidad Nacional Autónoma de México. Circuito Exterior, Ciudad Universitaria, Coyoacán, México, D.F.
*
*E-mail: [email protected] and [email protected]
*E-mail: [email protected] and [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The study evaluated the effect of storage time and conditions of nutritional pellets (NP) containing Duddingtonia flagrans chlamydospores on its in vitro trapping ability against Haemonchus contortus L3 larvae. The treated batch (200 NP) contained 4 ×  106 chlamydospores of the FTH0-8 strain, whereas the control batch (200 NP) was produced without spores. Both NP batches were exposed to four experimental storage conditions: (T1) shelves (indoors); (T2) refrigeration (4°C); (T3) outdoors under a roof; and (T4) 100% outdoors. Each group comprised 48 NP with spores and 48 NP without spores (control). The ability of D. flagrans spores to trap H. contortus L3 larvae was evaluated for 8 weeks for each storage condition. For that purpose, six randomly selected NP with spores were compared to their respective control NP. Each NP was individually crushed. The crushed material (1 g) was placed on the surface of a 2% water agar plate with 200 H. contortus L3 larvae. Plates were sealed and were incubated at room temperature for 8 days. The whole content of every plate was transferred to a Baermann apparatus to recover the remaining larvae. There was a clear larval reduction in the NP with spores, compared to the respective control NP in the four storage conditions (P< 0.05). The mean reductions ( ± SEM) of the storage conditions were 67 ± 4.9 (T2), 77 ± 6.1 (T1), 81.5 ± 3.8 (T4) and 82.1 ± 2.5 (T3). Larval reductions were similar at all times and were not affected by storage conditions or storage time (R2< 0.2; P>0.05). The long-term shelf-life of the chlamydospores in the NP suggests that this spore dosage technology is a viable option.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 1 , January 2015 , pp. 13 - 18
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Arroyo-Balán, F.L., Mendoza-de-Gives, P., López-Arellano, M.E., Liébano-Hernández, E., Vázquez-Pratz, V., Miranda-Miransa, E. & Ortiz-de-Montellano-Nolasco, A.M. (2008) Evaluación de un método combinado de control de la hemoncosis ovina en condiciones controladas. Técnica Pecuaria México 46, 217–223.Google Scholar
Casillas-Aguilar, J.A., Mendoza-de-Gives, P., López-Arellano, M.E. & Liébano-Hernández, E. (2008) Evaluation of multinutritional pellets containing Duddingtonia flagrans chlamydospores for the control of ovine haemonchosis. Annals of the New York Academy of Science 1149, 161–163.Google Scholar
Chandrawathani, P., Jammah, O., Waller, P.J., Hoglund, J., Larsen, M. & Zahari, W.M. (2002) Nematophagous fungi as a biological control agent for nematode parasites of small ruminants in Malaysia: a special emphasis on Duddingtonia flagrans. Veterinary Research 33, 685–696.Google Scholar
Chandrawathani, P., Jamnah, O., Adnan, M., Waller, P.J., Larsen, M. & Gillespie, A.T. (2003) Field studies on the biological control of nematode parasites of sheep in the tropics, using the microfungus Duddingtonia flagrans. Veterinary Parasitology 120, 177–187.CrossRefGoogle Scholar
Faedo, M., Larsen, M. & Waller, P.J. (1997) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: comparison between Australian isolates of Arthrobotrys spp. and Duddingtonia flagrans. Veterinary Parasitology 72, 149–155.CrossRefGoogle ScholarPubMed
Faedo, M., Barnes, E.H., Dobson, R.J. & Waller, P.J. (1998) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: pasture plot study with Duddingtonia flagrans. Veterinary Parasitology 72, 129–135.CrossRefGoogle Scholar
Larsen, M. (2006) Biological control of nematode parasites in sheep. Journal of Animal Science 84, E133–E139.CrossRefGoogle ScholarPubMed
Larsen, M., Faedo, M., Waller, P.J. & Hennessy, D.R. (1998) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: studies with Duddingtonia flagrans. Veterinary Parasitology 76, 121–128.Google Scholar
Llerandi-Juárez, R.D. & Mendoza-de-Gives, P. (1998) Resistance of chlamydospores of nematophagous fungi to the digestive processes of sheep in Mexico. Journal of Helminthology 72, 155–158.CrossRefGoogle Scholar
Mendoza-de-Gives, P., Flores-Crespo, J., Herrera-Rodríguez, D., Vázquez-Prats, V.M., Liébano-Hernández, E. & Ontiveros-Fernández, G.E. (1998) Biological control of Haemonchus contortus infective larvae in ovine faeces by administering an oral suspension of Duddingtonia flagrans chlamydospores to sheep. Journal of Helminthology 72, 343–347.CrossRefGoogle ScholarPubMed
Mendoza de Gives, P., Zapata-Nieto, C., Liébano Hernández, E., López-Arellano, E., Herrera Rodríguez, D. & González-Garduño, R. (2006) Biological control of gastrointestinal parasitic nematodes using Duddingtonia flagrans in sheep under natural conditions in Mexico. Annals of the New York Academy of Sciences 1081, 355–359.Google Scholar
Ojeda-Robertos, N.F., Mendoza-de-Gives, P., Torres-Acosta, J.F.J., Rodríguez-Vivas, R.L. & Aguilar-Caballero, A.J. (2005) Evaluating the effectiveness of a Mexican strain of Duddingtonia flagrans as a biological control agent against gastrointestinal nematodes in goats faeces. Journal of Helminthology 79, 151–157.Google Scholar
Ojeda-Robertos, N.F., Torres-Acosta, J.F.J., Aguilar-Caballero, A.J., Ayala-Burgos, A., Cob-Galera, L.A., Sandoval-Castro, C.A., Barrientos-Medina, R.C. & Mendoza-de-Gives, P. (2008) Assessing the efficacy of Duddingtonia flagrans chlamydospores per gram of faeces to control Haemonchus contortus larvae. Veterinary Parasitology 158, 329–335.Google Scholar
Paraud, C., Hoste, H., Lefrileux, Y., Pommaret, A., Paolini, V., Pors, I. & Chartier, C. (2005) Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials. Veterinary Research 36, 157–166.Google Scholar
Rubio, C. & Vidal, J. (2000) El bloque multi-nutricional (BMN). pp. 1–6in Investigación en Nutrición de Rumiantes. México, INIFAP.Google Scholar
Sagués, M.F., Fusé, L.A., Fernández, A.S., Iglesias, L.E., Moreno, F.C. & Saumel, A.C. (2011) Efficacy of an energy block containing Duddingtonia flagrans in the control of gastrointestinal nematodes of sheep. Proceedings of the 23rd International Conference of the WAAVP, Buenos Aires, Argentina, p. 27.CrossRefGoogle Scholar
Torres-Acosta, J.F.J., Sandoval-Castro, C.A., Hoste, H., Aguilar-Caballero, A.J., Cámara-Sarmiento, R. & Alonso-Díaz, M.A. (2012) Nutritional manipulation of sheep and goats for the control of gastrointestinal nematodes under hot humid and subhumid tropical conditions. Small Ruminant Research 103, 28–40.CrossRefGoogle Scholar
Waller, P.J. & Faedo, M. (1993) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: screening studies. Veterinary Parasitology 49, 285–297.Google Scholar
Waller, P.J., Knox, M.R. & Faedo, M. (2001a) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: feeding and block studies with Duddingtonia flagrans. Veterinary Parasitology 102, 321–330.Google Scholar
Waller, P.J., Faedo, M. & Ellis, K. (2001b) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: towards the development of a fungal controlled release device. Veterinary Parasitology 102, 299–308.CrossRefGoogle ScholarPubMed