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Digenean Holostephanus (Trematoda: Digenea: Cyathocotylidae) metacercariae in common carp (Cyprinus carpio Linnaeus, 1758) muscle: zoonotic potential and sensitivity to physico-chemical treatments

Published online by Cambridge University Press:  17 January 2020

D. Sándor Open the ORCID record for D. Sándor [Opens in a new window] ,
M. Gyöngy ,
K. Nyeste ,
I. Czeglédi ,
C. Székely ,
K. Buchmann  and
G. Cech
D. Sándor*
Affiliation:
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary Eötvös Loránd University, Doctoral School of Biology Program of Zootaxonomy, Animal Ecology and Hydrobiology, Budapest, Hungary
M. Gyöngy
Affiliation:
Department of Hydrobiology, University of Debrecen, Debrecen, Hungary Pál Juhász-Nagy Doctoral School of Biology and Environmental Sciences, University of Debrecen, Debrecen, Hungary
K. Nyeste
Affiliation:
Department of Hydrobiology, University of Debrecen, Debrecen, Hungary Pál Juhász-Nagy Doctoral School of Biology and Environmental Sciences, University of Debrecen, Debrecen, Hungary
I. Czeglédi
Affiliation:
Centre for Ecological Research, Balaton Limnological Institute, Hungarian Academy of Sciences, Tihany, Hungary
C. Székely
Affiliation:
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary
K. Buchmann
Affiliation:
Department of Veterinary and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
G. Cech
Affiliation:
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary
*
Author for correspondence: D. Sandor, E-mail: [email protected]
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Abstract

Metacercariae of various species within the genus Holostephanus Szidat, 1936 (Trematoda: Digenea: Cyathocotylidae) occur in muscles of both farmed and wild fish, including common carp (Cyprinus carpio Linnaeus, 1758). The life cycle includes a snail as first intermediate host, fish as second intermediate host and birds or mammals as final hosts. We studied the zoonotic potential and the viability of Holostephanus metacercariae from common carp following exposure to various physical and chemical treatments. Muscle tissue samples of common carp specimens from a fish farm in the north-eastern part of Hungary were examined and metacercariae recovered. The zoonotic potential was evaluated experimentally by using small mammals as models (albino mice, n = 2; and Syrian hamsters, n = 4) infected per os with Holostephanus cysts. Parallelly, Metagonimus metacercariae were used as positive controls. We could not confirm the zoonotic potential of Holostephanus metacercariae as they did not survive in the mammalian intestine whereas Metagonimus metacercariae developed to the adult stage. We assessed the viability of metacercariae isolated from common carp specimens during exposure to different physical treatments (temperatures of −18°C, +20°C, +40°C and +60°C) and chemical agents (5% and 10% acetic acid and 10% sodium chloride (NaCl)). Metacercariae lost viability by freezing at −18°C (2 h), heating at 60°C (20 min), incubation in 5% and 10% acetic acid (5 min) and 10% NaCl (2 h). These methods served as models to investigate the effectiveness of food preparation techniques (such as cold and hot smoking, freezing, salting and pickling) on the survival of metacercariae.

Keywords

Common carpdigenean trematodeszoonosisviabilitymuscle metacercariae
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e117
Copyright
Copyright © Cambridge University Press 2020

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