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The differentiation of parasitic nematodes using random amplified polymorphic DNA

Published online by Cambridge University Press:  05 June 2009

M.R. Chacón ,
E. Rodriguez ,
R.M.E. Parkhouse ,
P.R. Burrows  and
T. Garate
M.R. Chacón
Affiliation:
Department of Biochemistry, Wellcome Centre for Parasitic Infections, Imperial College of Science, Technology and Medicine, London, SW7 2AY, UK
E. Rodriguez
Affiliation:
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Departamento de Parasitología, 28220 Majadahonda, Madrid, Spain
R.M.E. Parkhouse
Affiliation:
Institute for Animal Health, Division of Immunology, Pirbright Laboratory Woking, GU24 0NF, UK
P.R. Burrows
Affiliation:
Rothamsted Experimental Station, Entomology and Nematology Department, Harpenden, Herts, AL5 2JQ, UK
T. Garate
Affiliation:
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Departamento de Parasitología, 28220 Majadahonda, Madrid, Spain
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Abstract

DNA from species and races of plant parasitic nematodes (Meloidogyne, Globodera and Heterodera) and a human parasitic nematode (Trichinella) were subjected to polymerase chain reaction amplification using one arbitrary primer (M-10). This technique results in relatively simple DNA profiles that include polymorphic markers known as random amplified polymorphic DNA (RAPDs). The RAPD profiles of the plant nematode species of Meloidogyne made possible the identification of M. incognita and M. hapla, but no differences were found between the patterns of M. javanica, M. arenaria and M. graminicola. Moreover, the four races of M. incognita were indistinguishable by this primer. In contrast, when races of the plant nematode Globodera rostochiensis (Ro1 and Ro2/3) were studied under the same RAPDs conditions, a race specific profile allows these two most devastating races to be differentiated. When DNAs of eight Trichinella isolates were subjected to RAPD studies, four different patterns were identified, corresponding to the four Trichinella clusters previously defined by isozyme polymorphism.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 2 , June 1994 , pp. 109 - 113
Copyright
Copyright © Cambridge University Press 1994

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