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Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis

Published online by Cambridge University Press:  25 March 2009

E. Hernández-Cruz ,
J.J. González-Cabriales ,
C. Ordaz-Pichardo ,
N.I. de la Cruz-Hernández  and
G.H. Flores-Gutiérrez
E. Hernández-Cruz
Affiliation:
Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México
J.J. González-Cabriales
Affiliation:
Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México
C. Ordaz-Pichardo
Affiliation:
Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México
N.I. de la Cruz-Hernández
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia ‘Dr. Norberto Treviño Zapata’, Universidad Autónoma de Tamaulipas, Victoria, Tamaulipas, México
G.H. Flores-Gutiérrez*
Affiliation:
Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México
*
*Fax: +52 899 923 0622 E-mail: [email protected]
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Abstract

An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P < 0.05, kappa = 0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.

Type
Research Papers
Information
Journal of Helminthology , Volume 83 , Issue 4 , December 2009 , pp. 333 - 337
Copyright
Copyright © Cambridge University Press 2009

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