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Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens

Published online by Cambridge University Press:  26 March 2010

S. Janardhan ,
P. Pandiaraja ,
V. Pandey ,
A. Karande  and
P. Kaliraj
S. Janardhan
Affiliation:
Centre for Biotechnology, Anna University, Chennai 600025, India
P. Pandiaraja
Affiliation:
Centre for Biotechnology, Anna University, Chennai 600025, India
V. Pandey
Affiliation:
Centre for Biotechnology, Anna University, Chennai 600025, India
A. Karande
Affiliation:
Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
P. Kaliraj*
Affiliation:
Centre for Biotechnology, Anna University, Chennai 600025, India
*
*Fax: +91-44-22350299 E-mail: [email protected]
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Abstract

The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the World Health Organization. Presently, few immunodiagnostics are available for filarial monitoring programmes. The Wuchereria bancrofti (Wb) SXP-1 parasite protein, with 84% homology to Brugia malayi (Bm) SXP-1, was found to be highly immunogenic. WbSXP-1 is one among the diagnostic candidate molecules that were used for developing a rapid-antibody-flow-through diagnostic kit for filariasis. Studies were initiated with the aim of developing monoclonal antibodies against recombinant WbSXP-1 and prospective applications for the detection of both circulating Wb and Bm antigens in serum samples from infected individuals. The monoclones 1A6C2 of subclass IgG1k, and 2A12F8 of class IgM, specifically detected Wb and Bm microfilaria isolated from patients and did not show cross-reactivity with other filarial recombinant antigens. We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.

Type
Research Papers
Information
Journal of Helminthology , Volume 85 , Issue 1 , March 2011 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 2010

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