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Determination of the minimum time of praziquantel therapy required for the in vitro treatment of protoscoleces of Echinococcus granulosus

Published online by Cambridge University Press:  05 June 2009

D. L. Morris ,
D. H. Taylor ,
D. Daniels ,
E. M. Riley  and
K. S. Richards
D. L. Morris
Affiliation:
Department of Surgery, University Hospital, Nottingham, NG7 2UH
D. H. Taylor
Affiliation:
Department of Surgery, University Hospital, Nottingham, NG7 2UH
D. Daniels
Affiliation:
Department of Biological Sciences, University of Keele, Staffs, ST5 5BG, England
E. M. Riley
Affiliation:
Department of Biological Sciences, University of Keele, Staffs, ST5 5BG, England
K. S. Richards
Affiliation:
Department of Biological Sciences, University of Keele, Staffs, ST5 5BG, England
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Abstract

Ovine and equine protoscoleces of Echinococcus granulosus were cultured for 26 days with our without praziquantel and viability assessed, by eosin exclusion, for cultures in various drug concentrations (50, 250 and 500 μg/l) and periods of exposure (1, 3 or 7 days (d)) before removing/‘rescuing’ to drug-free medium. Drug efficacy was proportional to drug concentration and to length of exposure. At higher drug concentrations shorter exposures were required to produce the effect of continuous drug treatment, 1d therapy at 500 μg/l killing 96% ovine protoscoleces by day 14 whereas 7d therapy at 50 μg/l was required to produce a similar effect. Equine protoscoleces appeared marginally less susceptible than those of ovine origin. The relevance of the results in the need for peri-operative prophylaxis against spilled protoscoleces in man is discussed.

Keywords

Echinococcus granulosusprotoscolecespraziquantel
Type
Research Papers
Information
Journal of Helminthology , Volume 62 , Issue 1 , March 1988 , pp. 10 - 14
Copyright
Copyright © Cambridge University Press 1988

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References

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