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Detection of circulating antigen in bancroftian filariasis by sandwich ELISA using filarial serum IgG

Published online by Cambridge University Press:  05 June 2009

M. V. R. Reddy ,
Ashok Malhotra  and
B. C. Harinath
M. V. R. Reddy
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha 442 102, India
Ashok Malhotra
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha 442 102, India
B. C. Harinath
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha 442 102, India
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Abstract

The utility of the IgG fraction of human filarial serum immunoglobulin in detecting circulating antigen by sandwich enzyme linked immunosorbent assay (ELISA) was studied. 27 of 33 sera from persons with microfilaracmia, 19 of 30 sera from clinical cases of filariasis, 4 of 30 sera from normal persons from a region endemic for filariasis showed the presence of circulating filarial antigen. All the 20 normal sera from the area where filariasis was not endemic gave negative reaction for filarial antigen. Those sera from persons with microfilaracmia that showed the presence of circulating antigen also showed an apparent positive correlation between the microfilarial density and the antigen titre.

Type
Research Article
Information
Journal of Helminthology , Volume 58 , Issue 3 , September 1984 , pp. 259 - 262
Copyright
Copyright © Cambridge University Press 1984

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