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A competitive enzyme-linked immunosorbent assay for rapid detection of antibodies against Trichinella spiralis and T. britovi – one test for humans and swine

Published online by Cambridge University Press:  23 November 2017

M. Gnjatovic ,
A. Gruden-Movsesijan ,
N. Miladinovic-Tasic ,
N. Ilic ,
S. Vasilev ,
J. Cvetkovic  and
Lj. Sofronic-Milosavljevic
M. Gnjatovic*
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
A. Gruden-Movsesijan
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
N. Miladinovic-Tasic
Affiliation:
Public Health Institute Niš, Blvd. Dr Zorana Djindjica 50, 18000 Nis, Serbia
N. Ilic
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
S. Vasilev
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
J. Cvetkovic
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
Lj. Sofronic-Milosavljevic
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
*
Author for correspondence: M. Gnjatovic, Fax: +381 2618 724, E-mail: [email protected]
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Abstract

Infection with parasites from the Trichinella genus occurs in many vertebrates but disease only occurs in humans (trichinellosis). Humans are infected after the consumption of raw or undercooked meat from infected wild or domestic animals (usually swine or horses). Using the monoclonal antibody (mAb) 7C2C5, specific for an epitope unique to the muscle larvae of the genus Trichinella, we have developed a competitive enzyme-linked immunosorbent assay (c-ELISA) that enables the rapid detection of Trichinella-specific antibodies in sera originating from two different host species (human, swine) infected with either Trichinella spiralis or Trichinella britovi. This novel c-ELISA exhibited 100% specificity and sensitivity, as confirmed by a Western blot test. The assay is easy to use (one incubation step), and the time required for the procedure (45 min) is shorter than in any other ELISA format. This test could be useful for both the detection and surveillance of Trichinella infections.

Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 1 , January 2019 , pp. 33 - 41
Copyright
Copyright © Cambridge University Press 2017 

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References

Akisu, C, Delibacs, SB and Ozkoc, S (2005) Evaluation of three different ELISA kits for the diagnosis of trichinellosis. Mikrobiyoloji Bülteni 39, 325–331.Google Scholar
Bruschi, F, Tassi, C and Pozio, E (1990) Parasite-specific antibody response in Trichinella sp. 3 human infection: a one year follow-up. American Journal of Tropical Medicine and Hygiene 43, 186–193.Google Scholar
Bruschi, F, Moretti, A, Wassom, D and Piergili Fioretti, D (2001) The use of a synthetic antigen for the serological diagnosis of human trichinellosis. Parasite 8, 141–143.Google Scholar
Debeljak, Z, Boufana, B, Interisano, M, Vidanovic, D, Kulisic, Z and Casulli, A (2016) First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia. Veterinary Parasitology 223, 57–62.Google Scholar
Djurkovic-Djakovic, O, Bobic, B and Klun, I (2010) Toxoplasmosis in Serbia: time for an action plan. Parasite 17, 187–192.Google Scholar
Dupouy-Camet, J and Bruschi, F (2007) Management and diagnosis of human trichinellosis. pp. 37–68 in Dupouy-Camet, J and Murrell, KM (Eds) FAO/WHO/OIE guidelines for the surveillance, management, prevention and control of trichinellosis. Paris, France, FAO/WHO/OIE.Google Scholar
Escalante, M, Romarís, F, Rodriguez, M, Rodriguez, E, Leiro, J, Gárate, MT and Ubeira, FM (2004) Evaluation of Trichinella spiralis larva group 1 antigens for serodiagnosis of human trichinellosis. Journal of Clinical Microbiology 42, 4060–4066.Google Scholar
Forbes, LB, Appleyard, GD and Gajadhar, AA (2004) Comparison of synthetic tyvelose antigen with excretory–secretory antigen for the detection of trichinellosis in swine using enzyme-linked immunosorbent assay. Journal of Parasitology 90, 835–840.Google Scholar
Gabrielli, S, Tasić-Otašević, S, Ignjatović, A, Fraulo, M, Trenkić-Božinović, M, Momčilović, S and Cancrini, G (2017) Seroprevalence and risk factors for Toxocara canis infection in Serbia during 2015. Foodborne Pathogens and Disease 14, 43–49.Google Scholar
Gamble, HR and Graham, CE (1984a) Comparison of monoclonal antibody-based competitive and indirect enzyme-linked immunosorbent assays for the diagnosis of swine trichinosis. Veterinary Immunology and Immunopathology 6, 379–389.Google Scholar
Gamble, HR and Graham, CE (1984b) Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. American Journal of Veterinary Research 45, 67–74.Google Scholar
Gamble, HR, Gajadhar, AA and Solomon, MB (1996) Methods for the detection of trichinellosis in horses. Journal of Food Protection 59, 420–425.Google Scholar
Gamble, HR, Pozio, E, Bruschi, F, Nockler, K, Kapel, CMO and Gajadhar, AA (2004) International Commission on Trichinellosis: recommendations on the use of serological tests for the detection of Trichinella infection in animals and man. Parasite 11, 3–13.Google Scholar
Gomez-Morales, MA, Ludovici, A, Amati, M, Cherchi, S, Pezzotti, P and Pozio, E (2008) Validation of an enzyme-linked immunosorbent assay for diagnosis of human trichinellosis. Clinical and Vaccine Immunology 15, 1723–1729.Google Scholar
Gottstein, B, Pozio, E and Nockler, K (2009) Epidemiology, diagnosis, treatment and control of trichinellosis. Clinical Microbiology Reviews 22, 127–145.Google Scholar
Hill, DE, Forbes, L, Gajadhar, AA and Gamble, HR (2007) Viability and infectivity of Trichinella spiralis muscle larvae in frozen horse tissue. Veterinary Parasitology 146, 102–106.Google Scholar
Ilic, N, Gruden-Movsesijan, A, Zivojinovic, M and Sofronic-Milosavljevic, L (2014) Characteristic band pattern in Western blots for specific detection of anti-Trichinella spiralis antibodies in different host species. Acta Veterinaria Belgrade 64, 33–43.Google Scholar
Jacobson, RH (1998) Validation of serological assays for diagnosis of infectious diseases. Revue Scientifique et Technique (International Office of Epizootics) 17, 469–486.Google Scholar
Jung, D, Teifke, JP, Karger, A, Michael, K, Venz, S, Wittmann, W, Kindermann, K, Nockler, K and Mundt, E (2007) Evaluation of baculovirus-derived recombinant 53-kDa protein of Trichinella spiralis for detection of Trichinella-specific antibodies in domestic swine by ELISA. Parasitology Research 100, 429–437.Google Scholar
Marti, E, Horohov, DW, Antzak, DF, Lazary, S and Lunn, DP (2003) Advances in equine immunology: Havemeyer workshop reports from Santa Fe, New Mexico, and Hortobagy, Hungary. Veterinary Immunology and Immunopathology 91, 233–243.Google Scholar
Nagano, I, Wu, Z and Takahashi, Y (2008) Species-specific antibody responses to the recombinant 53-kilodalton excretory and secretory proteins in mice infected with Trichinella spp. Clinical and Vaccine Immunology 15, 468–473.Google Scholar
Nockler, K, Pozio, E, Voigt, WP and Heidrich, J (2000) Detection of Trichinella infection in food animals. Veterinary Parasitology 93, 335–350.Google Scholar
Nockler, K, Serrano, FJ, Boireau, P, Kapel, CM and Pozio, E (2005) Experimental studies in swine on Trichinella detection in different diagnostic matrices. Veterinary Parasitology 132, 85–90.Google Scholar
Pozio, E (2007) World distribution of Trichinella spp. infections in animals and humans. Veterinary Parasitology 149, 3–21.Google Scholar
Pozio, E, Sofronic-Milosavljevic, L, Gomez-Morales, MA, Boireau, P and Nockler, K (2002) Evaluation of ELISA and Western blot analyses using three antigens to detect anti-Trichinella IgG in horses. Veterinary Parasitology 108, 163–178.Google Scholar
Radovic, I, Gruden-Movsesijan, A, Ilic, N, Mostarica-Stojkovic, M and Sofronic-Milosavljevic, L (2012) Trichinella spiralis shares epitopes with human autoantigens. Memórias do Instituto Oswaldo Cruz 107, 503–509.Google Scholar
Ribicich, M, Gamble, HR, Bolpe, J, Scialfa, E, Mundo, S, Pasqualetti, M, Cardillo, N, San Martin, C, Vizio, E, Borrás, P, Fariña, F and Rosa, A (2011) Diagnosis of trichinellosis in swine by ELISA test with three types of antigens of Trichinella spiralis in swine raised under different conditions of confinement. The Pig Journal 66, 55–58.Google Scholar
Ruangkunaporn, Y, Watt, G, Karnasuta, C, Jongsakul, K, Mahannop, P, Chongsanguan, M and Chaicumpa, W (2011) Immunodiagnosis of trichinellosis: efficacy of somatic antigen in early detection of human trichinellosis. Asian Pacific Journal of Allergy and Immunology 12, 39–42.Google Scholar
Sofronic-Milosavljevic, L, Cuperlovic, K, Ivanovska, D and Djordjevic, M (1988) Specific antibodies to Trichinella spiralis L1 ES products in artificially infected swine. Acta Veterinaria Belgrade 38, 119–128.Google Scholar
Sofronic-Milosavljevic, Lj, Ilic, N, Djordjevic, M, Savic, M, Gruden-Movsesijan, A, Cuperlovic, K and Murrell, KD (2005) Anti-Trichinella detected in chronically infected horses by IFA and Western blot, but not by ELISA. Veterinary Parasitology 132, 107–111.Google Scholar
WHO/OIE (World Organization for Animal Health) (2012a) Trichinellosis. WHO/OIE Manual of diagnostic tests and vaccines for terrestrial animals. Chapter 2.1.16. Paris, France, WHO/OIE.Google Scholar
WHO/OIE (World Organization for Animal Health) (2012b) Principles of validation of diagnostic assays for infectious diseases. Manual of diagnostic tests and vaccines for terrestrial animals. Chapter 1.1.5. Paris, France, WHO/OIE.Google Scholar
Wisdom, GB (2005) Conjugation of antibodies to horseradish peroxidase. Methods in Molecular Biology 295, 127–130.Google Scholar
Yang, Y, Caib, NY, Tongc, MW, Sunc, N, Xuana, YH, Kanga, YH, Valléed, I, Pascal, B, Chengc, S and Liu, MY (2016) Serological tools for detection of Trichinella infection in animals and humans. One Health 2, 25–30.Google Scholar
Zweig, MH and Campbell, G (1993) Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine. Clinical Chemistry 39, 561–577.Google Scholar