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Characterization of mitochondrial monoamine oxidase of Ascaridia galli

Published online by Cambridge University Press:  18 November 2009

S.K. Mishra ,
Abha Agarwal ,
Ramanuj Sen  and
S. Ghatak
S.K. Mishra
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow-226001, India
Abha Agarwal
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow-226001, India
Ramanuj Sen
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow-226001, India
S. Ghatak
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow-226001, India
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Abstract

Oxidative deamination of various biogcnic monoamines by Ascaridia galli monoaminc oxidase (MAO) was blocked by different mammalian MAO inhibitors, namely, iproniazid, trans-PcP, nialamide and pargyline and the blockade was observed to be time as well as concentration dependent. The binding of inhibitors with chick ascarid MAO was of the irreversible type and the nature of the inhibition was competitive. Pargyline showed lowest I50 (8µM) and K1 (12µM) values. Chlorgyline and deprenyl at 100µM concentration inhibited MAO by about 60 and 40% respectively, indicating the presence of both type A and type B MAO in A. galli.

Type
Research Article
Information
Journal of Helminthology , Volume 59 , Issue 2 , June 1985 , pp. 101 - 107
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Barbato, L. M. & Abood, L.C. (1963) Purification and properties of monoamine oxidase. Blochimica Biophysica Acta, 67, 531–541.CrossRefGoogle Scholar
Collins, G. G. S. & Sandler, M (1971) Human blood platelet monoamine oxidase. Biochemical Pharmacology, 20, 289–296.CrossRefGoogle Scholar
Felner, A & Waldmeier, P. C. (1979) Cumulative effects of irreversible MAO inhibitors in vivo. Biochemical Pharmacology, 28, 995–1002.CrossRefGoogle Scholar
Fuller, R. W.(1972) Selective inhibition of monoamine oxidase. Advances in Biochemical Psychopharma cology, 5, 339–354.Google Scholar
Green, A. L. & Haughton, T. M. (1961) A colorimetric method for the stimation of monamine oxidase. Biochemical Journal, 78, 172–175.CrossRefGoogle Scholar
Horita, A (1967) Cardiac monoamine oxidase in rat. Nature, 215, 411–412.CrossRefGoogle Scholar
Johnston, J. P. (1968) Some observations upon a new inhibitor of monoamine oxidase in brain tissue. Biochemical Pharmacology, 17, 1285–1297.CrossRefGoogle Scholar
Knoll, J (1976) In “Monoamine oxidase and its inhibition”. CI BA Foundation Symposium 39 (New Series) Elsevier, Excerpta Modica. North Holland, Amsterdam, Oxford, New York, pp. 135–161.Google Scholar
Knoll, J (1977) In “Neuronal concept today”(Szentagothai, J., Hamori, J. and Vizi, E.S. editors), Akademiai Kiado, Budapest, pp. 109–117.Google Scholar
Knoll, J. (1978) The pharmacology of selective irreversible monamine oxidase inhibitors. In “Enzyme activated irreversible inhibitors” (Seiler, N., Jung, M. J. & Koch-Weser, J. editors) pp. 253–269, Biomedical Press, New York.Google Scholar
Knoll, J, Ecsery, Z, Kelemen, K, Nievel, J, & Knoll, B (1965) Phenylisopropyl mcthylpropinylamine (E-250), a new spectrum psychic energizer. Archives of International Pharmacodynamics 155, 154.Google Scholar
Lowry, O. H., Rosenbrough, N. J., Farr, A.L. & Randall, R. J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle Scholar
Magyar, K, Satory, E & Knoll, J (1973) Symposium on pharmacological agents and biogenic amines in the nervous system, First Congress of the Hungarian Pharmacological Society, Vol. 1 (Knoll, J. general editor, Magyar, K. editor) Akadcmiai Kiado, Budapest pp. 107–122.Google Scholar
Mishra, S. K., Sen, Ramanuj & Ghatak, S. (1978) Monoamine oxidase and metabolitcs of biogenic amines in human ascaris. Indian Journal of Parasitology, 2, 153–155.Google Scholar
Mishra, S. K., Sen, Ramanuj & Ghatak, S (1983) Monoamine oxidase in adult Ascaridia galli. Journal of Helminthology, 57, 313–318.CrossRefGoogle Scholar
Moreno, M. S. & Barrett, J (1979) Monoamine oxidase in adult Hymenolepis diminuta (Cestoda). Parasitology, 78, 1–5.CrossRefGoogle Scholar
Nimmo-Smith, R. H. & Raison, C. G. (1968) Monoamine oxidase activity of Schistosoma mansoni. Comparative Biochemistry & Physiology, 24, 403–416.CrossRefGoogle Scholar
Sen, Ramanuj, Parmer, S. S. & Guha, S. R. (1968) Studies on monoamine oxidase of rat heart mitochondria I purification and properties of the enzyme. Acta Biologica et Medica Germanica, 21, 1–11.Google Scholar
Sen, Ramanuj & Gulia, S. R. (1969) Studies on monoamine oxidase of rat heart mitochondria II inhibition of monoamine oxidase by certain monoamine oxidase inhibitors. Acta Biologica et Medica Germanica, 23, 387–395.Google Scholar
Squires, R. F. (1972) Multiple forms of monoamine oxidase in intact mitochondria as characterized by selective inhibitors and thermal stability. A comparison of eight mammalian species. Advances in Biochemical Psychopharmacology, 5, 355–370.Google Scholar